Summary
The production of high-content fructo-oligosaccharides from sucrose by a crude FTF from a new strain of Penicillium isolated in our Laboratory was investigated. The optimum conditions for the production of the enzyme and for the enzymic reaction have been determined. It has been demonstrated that the crude enzyme acts as a mixed enzyme system of fructosyl transferase (FTF; Class 2 of Enzyme Nomenclature) and glycosidases (Class 3 of Enyme Nomenclature). Under optimum conditions: pH 5.5, temperature 55°C, sucrose concentration 750 g/l, enzyme concentration 5 FTF units/g sucrose, conversion yield up to 80% were obtained and high concentration of nystose (412 g/l) and fructofuranosyl-nystose (176 g/l) were accumulated.
Similar content being viewed by others
References
Barthomeuf C, Regerat F. and Pourrat H. (1991) J. Ferment. Bioeng. 72, 491–494.
Bradford H.M. (1976) Anal Biochem. 72, 248–254.
Hidaka H, Eida T., Takizawa T; Tokunaga T. and Tashiro Y. (1986) Bifidobacteria Microflora, 5, 37–50
Hidaka H., Hiramaya H. and Yamada K. (1991) J. Carbohydrate Chem. 10, 509–522.
Kohmoto T., Fukui F. Tanaka H. and Mitsuoka T. (1991) Agr. Biol. Chem. 55, 2157–2159
Muramitsu M., Kainuma S., Miwa T. and Nakakuki T. (1988) Agr. Biol. Chem. 32, 1303–1304.
Ohstsuka K., Hino S., Fukushima T., Ozawa O., Kanematzu T. and Uchita T. (1992) Biosci. Biotech. Biochem. 56, 1373–1377.
Patel V., Saunders G. and Bucke C. (1994) Biotechnol. Lett., 16, 1139–1144.
Prescott S.C. and Dunn. C.G. (1959) Industrial Microbiology, 3∘ ed, Mc Graw Hill Book Co, INC, New York, USA, p. 159.
Raper K.B. and Thom C. (1949) A manual of the Penicillia,Williams and Wilkins, Baltimore, USA.
Sano T., Ishikawa M., Nozawa Y., Noshi K. and Someya K. (1986) Proceedings from the 3rd Neosugar conference, Tokyo, pp 11–19.
Schin HJ and Yang J.W. (1994) Biotechnol. Lett. 16, 1157–1162.
Yamashita H., Kaiwa K. and Itakura H. (1984) Nutrition Research, 4, 961–966.
Yun J.W., Jung K.H., Jeon Y.J. and Lee J.H. (1992) J. Microbiol. Biotechnol., 2, 98–101.
Yun J.W and Song S.K. (1993) Biotechnol. Lett. 15, 573–576
Yun J.W., Lee M.G. and Song S.K. (1994) J. Ferment. Bioeng. 77, 159–163
Yun J.W., Lee M.G. and Song S.K. (1994) Biotechnol. Lett. 16, 1145–1150.
Wada K., Watanabe J., Mizutani, J;, Tomoda M., Suzuki H; and Saitoh Y. (1992) Nippon Nogeikagaku Kaishi, 66, 127–135.
Wada K., Watanabe J., Tanaka H. and Mitsuoka T (1991) Agr. Biol. Chem. 55, 2157–2159.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Barthomeuf, C., Pourrat, H. Production of high-content fructo-oligosaccharides by an enzymatic system from Penicillium rugulosum . Biotechnol Lett 17, 911–916 (1995). https://doi.org/10.1007/BF00127425
Issue Date:
DOI: https://doi.org/10.1007/BF00127425