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Characteristic features of the sonicated DNA of Agama agama agama L. (Reptilia, Agamidae) on hydroxyapatite columns, using mouse DNA as a reference

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Abstract

Hydroxyapatite column chromatography has been used to study some properties of the extensively sheared DNA of the Rainbow lizard, Agama agama agama. Reassociation studies show that the genome has a Cot1/2 of 370. Approximately 15% of the genome is highly repetitive in nature. This repetitive fraction is resolved into thermally stable and less stable fractions. The stable fraction has a base composition of 47% GC, higher than the 40.2% GC for the native DNA. This stable fraction is believed to be of recent origin.

Chromatography of the total DNA of the lizard with linear gradients of phosphate buffer containing 1 M urea resolves it into two components which were shown by thermal fractionation, also in the presence of 1 M urea, to vary in base composition. This behaviour may be characteristic of reptilian genomes and may be used as a basis for studying the structural organisation of the reptilian genome.

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Author notes

  1. R. O. Onuigbo

    Present address: Michael Okpara College of Agriculture, Owerri, Nigeria

Authors and Affiliations

  1. Department of Zoology, Obafemi Awolowo University, Ile-Ife, Nigeria

    J. A. Adegoke, B. O. Ighavini & R. O. Onuigbo

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  1. J. A. Adegoke
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  2. B. O. Ighavini
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  3. R. O. Onuigbo
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Adegoke, J.A., Ighavini, B.O. & Onuigbo, R.O. Characteristic features of the sonicated DNA of Agama agama agama L. (Reptilia, Agamidae) on hydroxyapatite columns, using mouse DNA as a reference. Genetica 83, 171–180 (1991). https://doi.org/10.1007/BF00126222

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  • DOI: https://doi.org/10.1007/BF00126222

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