Summary
Using a method of single muscle fibre analysis, we investigated the presence of RNA transcripts for various isoforms of the myosin heavy chain (MyoHC) gene in histochemically, immunohistochemically and electrophoretically characterized individual muscle fibres (n=65) from adult human vastus lateralis muscle. A cDNA clone isolated in this study was shown to contain the 3′ end of a previously uncharacterized human MyoHC gene which is expressed specifically in human fast IIA muscle fibres and we conclude that this clone contains part of the human fast IIA MyoHC gene. In all the fibres histochemically, immunohistochemically and electrophoretically characterized as containing the previously classified IIB MyoHC (n=23), it was shown that the human equivalent to the rat type IIX MyoHC gene is expressed. This observation was taken to suggest that the previously classified IIB muscle fibres in human muscle express a MyoHC isoform equivalent to the rat IIX, not the IIB, and would therefore be more accurately classified as IIX fibres.
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BOTTINELLI, R., SCHIAFFINO, S. & REGGIANI, C. (1991) Force-velocity relations and myosin heavy chain isoform compositions of skinned fibres from rat skeletal muscle. J. Physiol. 437, 655–72.
CHOMCZYNSKI, P & SACCHI, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156–9.
DENARDI, C., AUSONI, S., MORETTI, P., GORZA, L., VELLECA, M., BUCKINGHAM, M. & SCHIAFFINO, S. (1993) Type-2x-myosin heavy chain is coded by a muscle fiber type-specific and developmentally regulated gene. J. Cell Biol. 123, 823–35.
ELLER, M., STEDMAN, H. H., SYLVESTER, J. E., FERTELS, S. H., RUBINSTEIN, N. A., KELLYAM & SARKAR, S. (1989) Nucleotide sequence of full length human embryonic myosin heavy chain cDNA. Nucl. Acids Res. 17, 3591–2.
ENNION, S., YOUNG, A., MALBUT, K., VERHAAR, H., SIMPSON, H. & GOLDSPINK, G. (1994) Syringe needle aspiration of skeletal muscle. An alternative to muscle biopsy for some applications. Proc. Physiol. Soc. 479P, 159P.
GORZA, L. (1990) Identification of a novel type 2 fiber population in mammalian skeletal muscle by combined use of histochemical myosin ATPase and anti-myosin monoclonal antibodies. J. Histochem. Cytochem. 38, 257–65.
GUTH, L. & SAMAHA, F. J. (1972) Qualitative differences between actomyosin ATPase of slow and fast mammalian muscle. Exp. Neurol. 34, 465–75.
HARVEY, R. J., VEREUGDENHIL, E., ZAMAN, S. H., BHANDAL, N. S., USHERWOOD, P. N. R., BARNARD, E. A. & DARLINSON, M. G. (1991) Sequence of a functional invertebrate GABAA receptor subunit which can form a chimeric receptor with a vertebrate alpha subunit. EMBO J. 10, 3239–54.
HILL, A. V. (1950) The dimensions of animals and their muscular dynamics. Sci. Prog. 38 (150), 209–30.
JAENICKE, T., DIEDERICH, K. W., HAAS, W., SCHLEICH, J., LICHTER, P., PFORDT, M. & VOSBERG, H. P. (1990) The complete sequence of the human beta-myosin heavy chain gene and a comparative analysis of its product. Genomics 8, 194–206.
KARSCH-MIZRACHI, I., FEGHALI, R., SHOWS, T. B. & LEINWAND, L. A. (1990) Generation of a full-length human perinatal myosin heavy-chain-encoding cDNA. Geme 89, 289–94.
MOORMAN, A. F. M., BOERDE, P. A. J., LINDERS, M. & CHARLES, R. (1984) The histone HS varient in Xenopus leavis. Cell Differ. 14, 113–23.
NEMETH, P. M. & PETTE, D. (1980) The interrelationship of two systems of fiber classification in rat EDL muscle. J. Histochem. Cytochem. 28, 193.
PETTE, D. & STARON, R. S. (1990) Cellular and molecular diversities of mammalian skeletal muscle fibres. Rev. Physiol. Biochem. Pharmacol. 116, 1–76.
SAEZ, L. & LEINWAND, L. A. (1986) Characterization of diverse forms of myosin heavy chain expressed in adult human skeletal muscle. Nucl. Acids Res. 14, 2951–69.
SALVIATI, G., BETTO, R., DANIELIBETTO, D. & ZEVIANI, M. (1983) Myofibrillar-protein isoforms and sarcoplasmic-reticulum Ca2+-transport activity of single human skeletal muscle fibres. Biochem. J. J. 224, 215–25.
SANT'ANA PEREIRA, J. A. A. & MOORMAN, A. F. M. (1994) Do type IIB fibres of human muscle correspond to the IIX/D or to the IIB of rats? Proc. Physiol. Soc. 479P, 161P.
SANT'ANA PEREIRA, J. A. A., WESSELS, A., NYTMANS, L., MOORMAN, A. F. M. & SARGEANT, A. J. (1995) New method for the accurate characterization of single human skeletal muscle fibres demonstrates a relation between mATPase and MyHC expression in pure and hybrid fibre types. J. Muscle Res. Cell Motil., 16, 21–34.
STARON, R. S. (1991) Correlation between myofibrillar ATPase activity and myosin heavy chain composition in rabbit muscle fibers. Histochem., 96, 21–4.
STARON, R. S. & PETTE, D. (1986) Correlation between myofibrillar ATPase activity and myosin heavy chain composition in rabbit muscle fibers. Histochemistry 86, 19–23.
TERMIN, A., STARON, R. S. & PETTE, D. (1989) Myosin heavy chain isoforms in histochemically defined fiber types of rat muscle. Histochem. 92, 453–7.
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Ennion, S., Sant' Ana Pereira, J., Sargeant, A.J. et al. Characterization of human skeletal muscle fibres according to the myosin heavy chains they express. J Muscle Res Cell Motil 16, 35–43 (1995). https://doi.org/10.1007/BF00125308
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DOI: https://doi.org/10.1007/BF00125308