Previous studies showed that Cd++ inhibits EGF-induced DNA synthesis that not EGF-induced myc mRNA accumulation or amino acid incorporation into protein in serum-starved NRK-49F cells. In this study, flow cytometry was used to analyze the DNA and protein content of individual cells stimulated with Cd++ and/or epidermal growth factor (EGF). myc oncogene expression in these cells was also measured. It was found that, in both parental NRK-49F cells and in a clonal subpopulation, N1, Cd++ induces an hypertrophic response. In parental NRK-49F cells, however, lower doses of Cd++ (0.5 μM) induced more pronounced hypertrophic responses than did higher doses (4 μM); whereas in N1 cells, the Cd++-induced hypertrophic response shows a pattern of increasing response with doses of Cd++ from 0.5 to 4 μM. myc mRNA accumulation measured 2 hours after stimulation correlated with the hypertrophic responses in both NRK-49F cells and in N1 cells. The results show that Cd++-induced hypertrophy in NRK-49F cells is associated with increased myc oncogene mRNA accumulation, indicating that cell proliferation and cell hypertrophy may in part share common activation pathways.
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Abbreviations
- EGF:
-
Epidermal growth factor
- FCM:
-
Flow cytometric analysis
- FITC:
-
fluorescein isothiocyanate
- PI:
-
propidium iodide
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Tang, N., Enger, M.D. Cadmium induces hypertrophy accompanied by increased myc mRNA accumulation in NRK-49F cells. Cell Biol Toxicol 7, 401–411 (1991). https://doi.org/10.1007/BF00124074
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DOI: https://doi.org/10.1007/BF00124074