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Use of cell cultures for predicting the biological effects of mycotoxins

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Abstract

The risk presented by mycotoxins is a toxicological problem. As the data given by physico-chemical analysis may be difficult to translate in terms of toxicity, however, especially when considering multiple contamination, we have developed a system for toxicological analysis of mycotoxins using cell cultures of different origins. The response of several cell types to a number of well defined mycotoxins was obtained in three days. This approach allowed us to: demonstrate and quantify a toxic effect, define some organ specificity related to the preferential action on a particular cell type, and detect an immunosuppressive effect. The results indicate that the system can be used for toxicological screening and that it has a predictive value for the pathological effects of tested products.

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Abbreviations

DAS:

Diacetoxyscirpenol

DON:

Deoxynivalenol

SV40:

Simian virus 40

PHA:

Phytohaemagglutinin

IC 50:

Inhibiting concentration 50

LD 50:

Lethal dose 50

DMSO:

dimethylsulfoxide

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Authors and Affiliations

  1. Laboratoire de Pathologie Cellulaire de l'ER 278 (Reseau de toxicologie nutritionelle MRES), Institut de Recherches Scientifiques sur le Cancer, BP no 8, 94802, Villejuif, France

    Saida Robbana-Barnat, Christiane Lafarge-Frayssinet & Charles Frayssinet

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  1. Saida Robbana-Barnat
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  2. Christiane Lafarge-Frayssinet
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  3. Charles Frayssinet
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Robbana-Barnat, S., Lafarge-Frayssinet, C. & Frayssinet, C. Use of cell cultures for predicting the biological effects of mycotoxins. Cell Biol Toxicol 5, 217–226 (1989). https://doi.org/10.1007/BF00122655

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