Abstract
Giemsa and Ag-staining techniques and C-banding were performed on cytological preparations of 22 specimens of silversides (Odontesthes bonariensis) in order to extend the karyological knowledge on the family Atherinidae, to contribute cytotaxonomically to the systematics of the species and to identify possible cytogenetical markers of the introduced population.
The karyotype is conservative, 2n being 48, and 44 chromosomes being uniarmed. The remaining four biarmed chromosomes show intraindividual morphological variability. This variability is sex-independent. Ag-stanining techniques reveal that NORs are located on the short arms of biarmed chromosomes and that the morphological variability is partially due to NOR activity. C-banding revealed the associations between NORs and constitutive heterochromatin which could be responsible for some apparent structural differences. The differences found could be cytogenetical markers of the introduced population.
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References
Amemiya, C. T. & Gold, J. R., 1986. Chromomyein A3 strains nucleolus organizer regions of fish chromosomes. Copeia 1986 (1): 226–231.
Arai, R. & Koike, A., 1980. A karyotype study on two species of freshwater fishes transplanted into Japan. Bull. natn. Sci. Mus. Tokyo (Zool.) 6(4): 275–278.
Bickham, J. W., 1979. Banded karyotypes of 11 species of American bats (genus Myotis). Cytologia, 44: 789–797.
Croce, C. M., Talavera, P., Basilico, C. & Miller, O. J., 1977. Suppression of production of mouse 28S ribosomal RNA in mouse-human hybrids segregating mouse chromosomes. Proc. natn. Acad. Sci, U.S.A. 74: 694–697.
Foresti, F., Almeida Toledo, L. F. & Toledo, S. A., 1981. Polymorphic nature of nucleolus organizer regions in fishes. Cytogenet. Cell Genet. 31: 137–144.
Gajardo, G. & Arratia, G., 1980. Aspectos sistematicos de Basilichthys en Chile (Peces, Atherinidae) Arch. Biol. Med. exp. 13(1): 140.
Gajardo, G. & Arratia, G., 1981. Analisis de la diferenciacion morfologica y cromosomica de los pejerreyes chilenos (Peces: Atherinidiae). Arch. Biol. Med. exp. 14(1): 57.
Gold, J. R., 1984. Silver-staining and heteromorphism of chromosomal nucleolus organizer regions in North American cyprinid fishes. Copeia 1984: 133–139.
Howell, W. M. & Black, D. A. 1980. Controlled silver staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36: 1014–1015.
Mayr, B., Rab, P. & Kalat, M. 1986a. NORs and counterstainenhanced fluorescence studies in Cyprinidác of different ploidy level. Genetica 69: 111–118.
Mayr, B., Rab, P. & Kalat, M., 1986b. Localisation of NORs and counterstain-enhanced fluorescence studies in Salmo gairdneri and Salmo trutta (Pisces Salmonidae). Theor. appl. Genet. 71: 703–707.
Miller, D. A., Dev, V. G., Tantravahi, R. & Miller, O. J., 1976a. Suppression of human nucleolus organizer activity in mouse-human somatic hybrid cells. Expl Cell Res. 101: 235–243.
Miller, O. J., Miller, D. A., Dev, V. G., Tantravahi, R. & Croce, C. M., 1976b. Expression of human and suppression of mouse nucleolus organizer activity in mouse-human somatic cell hybrids. Proc. natn. Acad. Sci. U.S.A. 73: 4531–4534.
Moreira-Filho, O., Bertollo, L. A. C. & Galetti Jr., P. M., 1984. Structure and variability of nucleolar organizer regions in Parodontidae fish. Can. J. Genet. Cytol. 26(5): 564–568.
Ohno, S., 1974. Protochordata, Cyclostomata, and Pisces. In: B., John (ed.), Animal cytogenetics, vol. 4, Chordata 1, Borntraeger, Berlin: 1–92.
Perry, T. & Wolff, S., 1974. New Giemsa method for the differential staining of sister chromatids. Nature 251: 156–158.
Post, A., 1965. Vergleichende Untersuchungen der Chromosomenzahlen bei Süsswasser-Telcosteen. Z. zool. Syst. Evol. Forsch. 3(1–2): 47–93.
Schmid, M., 1982. Chromosome banding in Amphibia. VII. Analysis of the structure and variability of NORs in Anura. Chromosoma 87: 327–344.
Schubert, I. & Wobus, U., 1985. In situ hybridization confirms jumping nucleolus organizing regions in Allium. Chromosoma 92: 143–148.
Sola, L., Cataudella, S. & Capanna, L., 1981. New developments in vertebrate cytotaxonomy III. Karyology of bony fishes: A review. Genetica 54: 285–328.
Sola, L., Camerini, B. & Cataudella, S., 1984. Cytogenetics of Atlantic cels: C- and C-banding, nucleolus organizer regions, and DNA content. Cytogenet. Cell Genet. 38: 206–210.
Sumner, A. T., 1972. A simple technique for demonstrating contromeric heterochromatin. Expl Cell Res. 75: 304–306.
Tortonese, E., 1985. Scientific and practical interest of a family of bony fishes: the antherinids. Riv. Limnol. 10: 33–40.
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Research work supported by CNR, Italy. Special grant I.P.R.A. — Sub-project 1. Paper No. 1192.
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Sola, L., Natili, G.L. & Cataudella, S. Cytogenetical characterization of Odontesthes bonariensis (Pisces, Atherinidae), an Argentine species introduced in Italy. Genetica 77, 217–224 (1988). https://doi.org/10.1007/BF00122390
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DOI: https://doi.org/10.1007/BF00122390