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New invasion assay using endothelial cells grown on native human basement membrane

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A new invasion assay is introduced using endothelial cells grown on native human basement membrane (BM). The source of the BM was human amnion. The amnion is a uniform tissue composed of an epithelial layer resting on a continuous basement membrane overlying an avascular collagenous stroma. The epithelium was removed exposing the basement membrane (BM) surface. Human umbilical cord endothelium or bovine capillary endothelium were cultivated on the BM surface. Human squamous carcinoma cells were inoculated onto the BM surface in the presence or absence of the endothelial monolayer. Tumor cells attached readily to both the endothelial monolayer or the BM surface alone. Tumor cells which invaded the basement membrane and underlying collagenous connective tissue were collected on a Millipore filter applied to the opposite side of the amnion. Tumor cells invaded the devitalized amnion connective tissue in the absence of endothelium. The presence of either bovine or human endothelium significantly reduced the rate of tumor cell invasion. This system should be useful for further quantitative studies of the interaction between endothelium and tumor cells with regard to the mechanism of invasion.

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References

  1. Baserga, R., Kisieleski, W. E., and Halvorsen, K., 1960, A study on the establishment and growth of tumor metastases with tritiated thymidine. Cancer Research, 20, 910–917.

    Google Scholar 

  2. Bogenmann, E., and Sordat, B., 1979, Interactions of tumor cells with endothelial cell monolayer grown on collagen gels. Journal of Cell Biology, 83, 79a.

    Google Scholar 

  3. Coman, D. R., 1953, Mechanisms responsible for the origin and distribution of blood borne tumor metastases. A review. Cancer Research, 13, 397–404.

    Google Scholar 

  4. Dingemans, D. P., Roos, E., Van Den Bergh-Weerman, M. A., and Van de Pavert, I. V., 1978, Invasion of liver tissue by tumor cells and leukocytes: comparative ultrastructure. Journal of the National Cancer Institute, 60, 583–590.

    Google Scholar 

  5. Fidler, I. J., 1973, The relationship of embolic homogeneity, number, size and viability to the incidence of experimental metastasis. European Journal of Cancer, 9, 223–227.

    Google Scholar 

  6. Foltz, C. M., Siegal, G. P., Russo, R. G., Terranova, V. P., and Liotta, L. A., 1981, Interactions of tumor cells with whole basement membrane in the presence or absence of endothelium. In: Proceedings of Chesapeak Conference on Hemostasis and Malignancy. Abstract.

  7. Jaffe, E. A., Hover, L. W., and Nachman, R. L., 1973 a, Synthesis of antihemophilic factor antigen by cultured human endothelial cells. Journal of Clinical Investigation, 52, 2757–2764.

    Google Scholar 

  8. Jaffe, E. A., Nachman, R. L., Becker, C. G., and Mimck, C. R., 1973 b, Culture of human endothelial cells derived from umbilical veins. Journal of Clinical Investigation, 52, 2745–2756.

    Google Scholar 

  9. Jones, D. S., Wallace, A. C., and Fraser, E. E., 1971, Sequence of events in experimental metastases of Walker 256 tumor: light, immunofluorescent and electron microscopic observations. Journal of the National Cancer Institute, 46, 493–504.

    Google Scholar 

  10. Jones, P. A., 1979 a, Construction of an artificial blood vessel wall from cultured endothelial and smooth muscle cells. Proceedings of the National Academy of Sciences, U.S.A., 76, 1882–1886.

    Google Scholar 

  11. Jones, P. A., Scott-Burden, T., and Gevers, W., 1979 b, Glycoprotein, elastin and collagen secretion by rat smooth muscle cells. Proceedings of the National Academy of Science, U.S.A., 76, 353–357.

    Google Scholar 

  12. Jones, P. A., and Declerck, Y. A., 1980, Destruction of extracellular matrices containing glycoproteins, elastin and collagen by metastatic human tumor cells. Cancer Research, 40, 3222–3227.

    Google Scholar 

  13. Jones, P. A., Nuestein, H. B., Gonzales, F., and Bogenmann, E., 1981, Invasion of an artifical blood vessel wall by human fibrosarcoma cells. Cancer Research, 41 4613–4620.

    Google Scholar 

  14. Kefalides, N. A., and Denduchis, B., 1969, Structural components of epithelial and endothelial basement membranes. Biochemistry, 8, 4613–4621.

    Google Scholar 

  15. Kinjo, M., 1978, Lodgment and extravasation of tumor cells in blood-borne metastasis: an electron microscope study. British Journal of Cancer, 38, 293–301.

    Google Scholar 

  16. Kleinman, H. K., Klebe, R. J., and Martin, G. R., 1981, Role of collagenous matrices in the adhesion and growth of cells. Journal of Cell Biology, 88, 473–485.

    Google Scholar 

  17. Kramer, R. H., and Nicolson, G. L., 1979 a, Interactions of tumor cells with vascular endothelial cell monolayers: A model for metastatic invasion. Proceedings of the National Academy of Sciences, U.S.A., 76, 5704–5708.

    Google Scholar 

  18. Kramer, R. H., and Nicolson, G. L., 1979 b, Metastatic tumor cells induce the in vitro degradation of endothelial cell extracellular matrix. Journal of Supramolecular Structures, Suppl., 3, 181.

    Google Scholar 

  19. Kramer, R. H., Gonzales, R., and Nicolson, G. L., 1980, Metastatic tumor cells adhere preferentially to the extracellular matrix underlying vascular endothelial cells. International Journal of Cancer, 26, 639–645.

    Google Scholar 

  20. Liotta, L. A., Abe, S., Robey, P. G., and Martin, G. R., 1979, Preferential digestion of basement membrane collagen by an enzyme derived from a metastatic murine tumor. Proceedings of the National Academy of Sciences, U.S.A., 76, 2268–2272.

    Google Scholar 

  21. Liotta, L. A., Tryggvason, K., Garbisa, S., Hart, I. R., Foltz, C. M., and Shafie, S., 1980a, Metastatic potential correlates with enzymatic degradation of basement membrane collagen. Nature, London, 284, 67–68.

    Google Scholar 

  22. Liotta, L. A., Garbisa, S., and Tryggvason, K., 1982, Biochemical mechanisms involved in tumor cell penetration of the basement membrane. Tumor Invasion and Metastases, edited by L. A. Liotta and I. R. Hart (The Hague, Boston and London: Martinus and Nijhoff Publisher), 319–333.

    Google Scholar 

  23. Long, D. M., 1979, Capillary ultrastructure in human metastasic brain tumors. Journal of Neurosurgery, 51, 53–58.

    Google Scholar 

  24. Ludatscher, R. M., Luse, S. A., and Suntzeff, V., 1967, An electron microscopic study of pulmonary tumor emboli from transplantable Morris hepatoma 5123. Cancer Research, 27, 1939–1952.

    Google Scholar 

  25. Posts, G., Doll, J., Hart, I. R., and Fidler, I. J., 1980, In vitro selection of murine B16 melanoma variants with enhanced tissue-invasive properties. Cancer Research, 40, 1636–1644.

    Google Scholar 

  26. Roos, E., and Dingemans, K. P., 1979, Mechanisms of metastasis. Biochemica et biophysica acta, 560, 135–166.

    Google Scholar 

  27. Russo, R. G., Liotta, L. A., Thorgeirsson, U., Brundage, R., and Schiffman, E., 1981, Polymorphonuclear leukocyte migration through human amnion membrane. Journal of Cell Biology, 91, 459–467.

    Google Scholar 

  28. Russo, R. G., Thorgeirsson, U., and Liotta, L. A., 1982, In vitro quantitative assay of invasion using human amnion. Tumor Invasion and Metastases, edited by L. A. Liotta and I. R. Hart (The Hague, Boston and London: Martinus and Nijhoff Pub), 173–187.

    Google Scholar 

  29. Salsbury, A. J., Burrage, K., and Hellmann, K., 1974, Histological analysis of the antimetastatic effect of (±)-1,2-bis(3,5-dioxopiperazin-1-yl) propane. Cancer Research, 34, 843–849.

    Google Scholar 

  30. Schwartz, A. L., Forster, C. S., Smith, P. A., and Liggins, G. C., 1977, Human amnion metabolism I. In vitro maintenance. American Journal of Obstetrics and Gynecology, 127, 470–474.

    Google Scholar 

  31. Sindelar, W. F., Tralka, T. S., and Ketcham, A. S., 1975, Electron microscopic observations on formation of pulmonary metastases. Journal of Surgical Research, 18, 137–161.

    Google Scholar 

  32. Sugarbaker, E. V., and Ketcham, A. S., 1977, Mechanisms and prevention of cancer dissemination: an overview. Seminars in Oncology, 4, 19–32.

    Google Scholar 

  33. Terranova, P. V., Liotta, L. A.,, Russo, R. G., and Martin, G. R., 1982, Role of laminin in the attachment and metastasis of tumor cells. Cancer Research, 42, 2265–2269.

    Google Scholar 

  34. Vracko, R., 1974, Basal lamina scaffold-anatomy and significance for maintenance of orderly tissue structure. American Journal of Pathology, 77, 314–338.

    Google Scholar 

  35. Warren, B. A., 1973, Environment of the blood-borne tumor embolus adherent to vessel wall. Journal of Medicine, 4, 150–177.

    Google Scholar 

  36. Warren, B. A., 1976, Some aspects of blood-borne tumor emboli associated with thrombosis. Zeitschrift für Krebsforschung, 81, 1–15.

    Google Scholar 

  37. Warren, B. A., Shubik, P., and Feldman, R., 1978, Metastasis via the blood stream: The method of intravasation of tumor cells in a transplantable melanoma of the hamster. Cancer Letter, 4, 245–251.

    Google Scholar 

  38. Warren, B. A., 1981, Cancer cell-endothelial reactions: The microinjury hypothesis and localized thrombosis in the formation of micrometastases. Malignancy and the Hemostatic system, edited by M. B. Donati et al. (New York: Raven Press), pp. 5–25.

    Google Scholar 

  39. Wood, S., Jr., 1958, Pathogenesis of metastasis formation observed in vivo in the rabbit ear chamber. Archives of Pathology, 66, 550–568.

    Google Scholar 

  40. Zamora, P. O., Danielson, K. G., and Hosick, H. L., 1980, Invasion of endothelial cell monolayers on collagen gels by cells from mammary tumor spheroids. Cancer Research, 40, 4631–4639.

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of Pathophysiology, National Cancer Institute, USA

    R. G. Russo & L. A. Liotta

  2. Laboratory of Chemistry, National Institute of Arthritis, Metabolism and Digestive Diseases, National Institutes of Health, 20205, Bethesda, Maryland, U.S.A.

    C. M. Foltz

Authors
  1. R. G. Russo
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  2. C. M. Foltz
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  3. L. A. Liotta
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Russo, R.G., Foltz, C.M. & Liotta, L.A. New invasion assay using endothelial cells grown on native human basement membrane. Clin Exp Metast 1, 115–127 (1983). https://doi.org/10.1007/BF00121491

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  • DOI: https://doi.org/10.1007/BF00121491

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