This study reports in vitro biocompatibility screening of different kinds of silane-treated hydroxyapatite (HA) powders, for use as modified filler in resorbable composite. The silane coupling agents investigated were all methoxysilanes, with either vinyl (VS), or methacryloxy (MPS), or primary amine (AMMO), or secondary amine (TRIAMO), or diamine (DAS) functionality. Evaluation of cell adhesion on the different silanized HA surfaces, indirect agar-overlay test and direct contact test on extracts showed the acute toxicity of all five free silane agents, the innocuity of strongly adsorbed silane molecules on HA surfaces and the dose-dependent toxicity of leachable silanol molecules.
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References
W. BONFIELD, J. Biomed. Eng. 210 (1988) 522.
C. DOYLE, K. E. TANNER and W. BONFIELD, Biomaterials 12 (1991) 841.
S. HIGHASI, T. YAMAMURO, T. NAKAMURA, Y. IKADA, S. H. HYON and K. JAMSHIDI, Biomaterials 7 (1986) 183.
A. VAN SLIEDREGT, PhD thesis' Leiden, Haveka b.v., Alblasserdam (1993).
K. VERHEYEN, PhD thesis' Leiden, Haveka b.v., Alblasserdam (1993).
K. P. ANDRIANO, A. U. DANIELS and J. HELLER, J. Applied Biomat. 3 (1992) 191.
K. P. ANDRIANO, A. U. DANIELS and J. HELLER, J. Applied Biomat. 3 (1992) 197.
S. N. KHORASINI, S. DEB, J. C. BEHIRI, M. BRADEN and W. BONFIELD, Bioceramics 5 (1986) 225.
E. P. PLUEDDEMANN, “Silane coupling agents” (Plenum Press, New York, 1982).
A. M. P. DUPRAZ, J. R. De WIJN, S. A. T. v. d. MEER and K. De GROOT, J. Biomed. Mater. Res. 30 (1996) 231.
S. EINARSSON, B. JOSEFSSON and S. Lagerkvist, J. Chromatography 282 (1983) 609.
S. EINARSSON, S. FOLESTAD, B. JOSEFSSON and S. LAGERKVIST, Anal. Chem. 58 (1986) 1638.
W. L. GUESS, S. A. ROSENBLUTH, B. SCHMIDT and J. AUTIAN, J. Pharm. Sci. 54 (1965) 156.
J. AUTIAN, Artif. Org. 1 (1977) 53.
O. SUDILOVSKY, Tissue Culture Association Manual 3 (1977) 607.
C. WAYMOUTH, In vitro 6 (1970) 109.
H. J. JOHNSON, S. J. NORTHUP, P. A. SEAGRAVES, M. ATALLAH, P. J. GARVIN, L. LIN and T. D. DARBY, J. Biomed. Mater. Res. 19 (1985) 489.
F. GRINNELL, Int. Rev. Cytol. 53 (1978) 65.
H. ISHIDA. Polymer Composites 5 (1984) 101.
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Dupraz, A.M.P., v. d. Meer, S.A.T., De Wijn, J.R. et al. Biocompatibility screening of silane-treated hydroxyapatite powders, for use as filler in resorbable composites. J Mater Sci: Mater Med 7, 731–738 (1996). https://doi.org/10.1007/BF00121408
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DOI: https://doi.org/10.1007/BF00121408