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Osteoinduction within PEO/PBT copolymer implants in cranial defects using demineralized bone matrix

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This study was undertaken to assess the osteoinductive effect addition of demineralized bone matrix (DBM) gel has, on the behaviour of osteoconductive bone-bonding PEO/PBT copolymer (PolyactiveR) implants. Cranial defects in rats were filled with these composites to study bone formation in comparison with several controls after 2 and 8 weeks survival time. Osteogenesis was qualitatively evaluated by using light- and transmission electron microscopy as well as backscatter electron imaging. Quantification of the amount of bone ingrowth was performed by using a computerized image analysis system. Initially, rapid calcification was observed in the polymer and DBM, followed by formation of new trabecular bone around the demineralized bone fragments. Bone ingrowth in implants consisting of plain copolymer was less than expected based on previous research, but the addition of demineralized bone matrix gel resulted in a significantly greater amount of new bone formation in the defects. We concluded that the application of DBM-gel to PolyactiveR implants had a beneficial effect on the amount of new bone formation in this material. This procedure combines the osteoinductive potential of DBM with the mechanical and bone-bonding properties of a copolymer, thus opening the way to the development of a line of osteoactive composite implants with good surgical handling properties.

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Authors and Affiliations

  1. Biomaterials Research Group, University of Leiden, Rijnsburgerweg 10, 2333 AA, Leiden, The Netherlands

    R. M. Van Haastert, J. J. Grote & C. A. Van Blitterswijk

  2. Osteotech Inc., 1151 Shrewsbury Avenue, 07702, Shrewsbury, N.J., USA

    A. B. Prewett

Authors
  1. R. M. Van Haastert
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  2. J. J. Grote
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  3. C. A. Van Blitterswijk
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  4. A. B. Prewett
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ENT-department, University Hospital Leiden

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Van Haastert, R.M., Grote, J.J., Van Blitterswijk, C.A. et al. Osteoinduction within PEO/PBT copolymer implants in cranial defects using demineralized bone matrix. J Mater Sci: Mater Med 5, 764–769 (1994). https://doi.org/10.1007/BF00120373

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