The cytotoxicity of neurotoxic agents was determined for a series of brain-derived cell types and compared with their toxic effects on BALB/c 3T3 fibroblasts, using the neutral red assay. Ranking of toxicants according to their potencies was the same for all cells tested and was in the order of methylmercury>cadmium> mercury>zinc>acrylamide. For a series of di- and triorganotins the ranking order was dibutyl>diphenyl>dibenzyl>diproyl> diethyl>dimethyltin and triphenyl>tribenzyl>trimethyltin, respectively. The test was sensitive enough to detect structure activity relationships between the degree of toxicity and the hydrophobic characteristics of the agents tested.
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Abbreviations
- Be2Sn:
-
dibenzyltin dichloride
- Be3Sn:
-
triphenyltin dichloride
- Bu2Sn:
-
dibutyltin dichloride
- CdCl2 :
-
cadmium chloride
- CH3HgCl:
-
methylmercuric chloride
- DMEM:
-
Dulbecco's Modified Eagle's Medium
- Et2Sn:
-
diethyltin dichloride
- FBS:
-
fetal bovine serum
- HgCl2 :
-
mercuric chloride
- Me2Sn:
-
dimethyltin dichloride
- Me3Sn:
-
trimethyltin hydroxide
- Phe2Sn:
-
diphenyltin dichloride
- Phe3Sn:
-
triphenyltin hydroxide
- Pr2Sn:
-
dipropyltin dichloride
- ZnCl2 :
-
zinc chloride
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Borenfreund, E., Babich, H. In vitro cytotoxicity of heavy metals, acrylamide, and organotin salts to neural cells and fibroblasts. Cell Biol Toxicol 3, 63–73 (1987). https://doi.org/10.1007/BF00117826
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DOI: https://doi.org/10.1007/BF00117826