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Lewis lung carcinoma (3LL) cells treated in vitro with ultraviolet radiation show reduced metastatic ability due to an augmented immunogenicity

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Abstract

The metastatic ability of 3LL tumor following in vitro irradiation with ultraviolet (u.v.) light was studied. Tumor cells were exposed to two courses of u.v.-irradiation (3LL × 2u.v. cells) and after two weeks of culture they were inoculated intravenously (i.v.) into syngeneic mice. These cells produced significantly fewer pulmonary metastases than the untreated population. In addition, intrafootpad (i.f.p.) injections of 3LL × 2u.v. cells into immunocompetent animals induced tumors only in 40 per cent of recipients. Interestingly, in normal mice with progressively growing 3LL × 2u.v. tumors, the formation of spontaneous pulmonary metastases was prevented, whereas metastatic foci were observed in 70 per cent of the nude recipients. The metastatic properties of u.v.-treated tumor cells were further analysed by using individual clones with varying immunogenicity. We found that variants with augmented immunogenicity also showed a parallel decrease in metastatic potential. Studies on H-2 antigen expression in different clones revealed that immunogenic and low metastatic variants expressed levels of H-2 antigens higher than the tumorigenic and metastatic clones. Finally, by using cyclophosphamide (Cy) treatment and adoptive transfer of immune spleen cells we were able to eradicate macroscopic 3LL pulmonary metastasis. These results demonstrate that the decrease of metastatic ability in u.v.-treated cells was mainly due to an increase in their immunogenicity and H-2 antigen expression.

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Author notes

  1. Ronald B. Herberman & Elieser Gorelik

    Present address: Pittsburgh Cancer Institute, 230 Lothrop Street, 15213, Pittsburgh, PA, U.S.A.

Authors and Affiliations

  1. Laboratory of Experimental Immunology, Biological Response Modifiers Program, Division of Cancer Treatment, National Cancer Institute, NIH, Frederick Cancer Research Facility, Building 560, Room 31-93, 21701, Frederick, MD, U.S.A.

    Samuele Peppolonii, Ronald B. Herberman & Elieser Gorelik

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  1. Samuele Peppolonii
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  2. Ronald B. Herberman
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  3. Elieser Gorelik
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Peppolonii, S., Herberman, R.B. & Gorelik, E. Lewis lung carcinoma (3LL) cells treated in vitro with ultraviolet radiation show reduced metastatic ability due to an augmented immunogenicity. Clin Exp Metast 5, 43–56 (1987). https://doi.org/10.1007/BF00116625

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  • DOI: https://doi.org/10.1007/BF00116625

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