Abstract
Callus was induced from juice vesicles of satsuma mandarin on Murashige & Skoog medium supplemented with α-naphthaleneacetic acid (NAA), kinetin (K) and gibberellin (GA). Adventitious embryoids arose from the callus tissue on the medium containing 1 mgl−1 NAA alone. The embryoids grew into embryos which resulted in a plantlet on medium containing 1 mgl−1 GA.
Abbreviations
- GA:
-
gibberellin
- K:
-
kinetin
- NAA:
-
α-naphthaleneacetic acid
References
Barlass M, Skene KGM (1982) In vitro plantlet formation from Citrus species and hybrids. Sci Hortic 17: 333–341
Cameron JW, Soost RK, Olson EO (1964) Chimeral basis for color in pink and red grapefruit. J Hered 55: 23–28
Chaturvedi HC, Mitra GC (1974) Clonal propagation of citrus from somatic callus cultures. HortScience 9: 118–120
Edriss MH, Burger DW (1984) In vitro propagation of ‘Troyer’ citrange from epicotyl segments. Sci Hortic 23: 159–162
Einset JW (1978) Citrus tissue culture. Stimulation of fruit explant cultures with orange juice. Plant Physiol 62: 885–888
Frost HB, Krug CA (1942) Diploid-tetraploid periclinal chimeras as bud variants in citrus. Genetics 27: 619–634
Hidaka T (1984) Induction of plantlet from anthers of ‘Trovita’ orange (Citrus sinensis Osbeck). J Jpn Soc Hortic Sci 53: 1–5
Hidaka T, Kajiura I (1988) Plantlet differentiation from callus protoplasts induced from Citrus embryo. Sci Hortic 34: 85–92
Hidaka T, Yamada Y, Shichijo T (1982) Plantlet formation by anther culture of Citrus aurantium L. Jpn J Breed 32: 247–252
Iwamasa M, Nishiura M (1970) Evidence for the chimeral nature of vegetative reversion in Suzuki Wase, an early ripening satsuma mandarin. Sabrao Newslett 2: 109–114
Kato Y (1980) Studies on juice vesicles isolated from mature and immature citrus fruit. I. Callus induction and cell differentiation in Citrus hassaku Hort. ex Tanaka. J Jpn Soc Hortic Sci 49: 36–40
Kobayashi S, Ikeda I, Nakatani I (1984) Induction of nucellar callus from orange (Citrus sinensis Osb.) ovules, and uniformity of regenerated plants. Bull Fruit Tree Res Stn Series E (Akitsu) 5: 43–54
Kobayashi S, Ohgawara T, Ohgawara E, Oiyama I, Ishii S (1988) A somatic hybrid plant obtained by protoplast fusion between navel orange (Citrus sinensis) and satsuma mandarin (C. unshiu). Plant Cell Tissue Organ Culture 14: 63–69
Kobayashi S, Uchimiya H, Ikeda I (1983) Plant regeneration from ‘Trovita’ orange protoplasts. Jpn J Breed 33: 119–122
Kochba J, Spiegel-Roy P, Safran H (1972) Adventive plants from ovules and nucelli in Citrus. Planta 106: 237–245
Kordan HA (1963) Growth characteristics of citrus fruit tissue in vitro. Nature 4883: 867–869
Kulshrestha VK, Chauhan YS, Roberts LW (1982) In vitro studies on xylogenesis in Citrus fruit vesicles. I. Effects of auxin, cytokinin and citric acid on induction of xylem differentiation. Phytomorphology 32: 14–17
Ling JT, Nito N, Iwamasa M (1989) Plant regeneration from protoplast of Calamondin (Citrus madurensis Lour.) Sci Hortic 40: 325–333
Machida H, Ooishi A, Hosoi T, Kawai H (1974) Clonal multiplication of citrus through tissue culture. Rep Fac Agric Shizuoka Univ 24: 15–21
Murashige T, Nakano R, Tucker DPH (1967) Histogenesis and rate of nuclear change in Citrus limon tissue in vitro. Phytomorphology 17: 469–476
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15: 473–497
Murashige T, Tucker DPH (1969) Growth factor requirements of citrus tissue culture. Proc 1st Int Citrus Symp 3: 1155–1161
Ohgawara T, Kobayashi S, Ohgawara E, Uchimiya H, Ishii S (1985) Somatic hybrid plants obtained by protoplast fusion between Citrus sinensis and Poncirus trifoliata. Theor Appl Genet 71: 1–4
Unger JW, Feng KA (1978) Growth and differentiation of juice vesicles of orange grown in vitro. Am J Bot 65: 511–515
Vardi A (1981) Protoplast derived plants from different citrus species and cultivars. Proc Int Soc Citriculture 1981 1: 149–152
Vardi A, Breiman A, Glun E (1987) Citrus cybrids: production by donor-recipient protoplast-fusion and verification by mitochondrial-DNA restriction profiles. Theor Appl Genet 75: 51–58
Vardi A, Spiegel-Roy P, Glun E (1975) Citrus cell culture: isolation of protoplasts, plating densities, effect of mutagens and regeneration of embryoids. Plant Sci Lett 4: 231–236
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Nito, N., Iwamasa, M. In vitro plantlet formation from juice vesicle callus of satsuma (Citrus unshiu Marc.). Plant Cell Tiss Organ Cult 20, 137–140 (1990). https://doi.org/10.1007/BF00114712
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DOI: https://doi.org/10.1007/BF00114712