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In vitro plantlet formation from juice vesicle callus of satsuma (Citrus unshiu Marc.)

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Abstract

Callus was induced from juice vesicles of satsuma mandarin on Murashige & Skoog medium supplemented with α-naphthaleneacetic acid (NAA), kinetin (K) and gibberellin (GA). Adventitious embryoids arose from the callus tissue on the medium containing 1 mgl−1 NAA alone. The embryoids grew into embryos which resulted in a plantlet on medium containing 1 mgl−1 GA.

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Abbreviations

GA:

gibberellin

K:

kinetin

NAA:

α-naphthaleneacetic acid

References

  1. Barlass M, Skene KGM (1982) In vitro plantlet formation from Citrus species and hybrids. Sci Hortic 17: 333–341

    Google Scholar 

  2. Cameron JW, Soost RK, Olson EO (1964) Chimeral basis for color in pink and red grapefruit. J Hered 55: 23–28

    Google Scholar 

  3. Chaturvedi HC, Mitra GC (1974) Clonal propagation of citrus from somatic callus cultures. HortScience 9: 118–120

    Google Scholar 

  4. Edriss MH, Burger DW (1984) In vitro propagation of ‘Troyer’ citrange from epicotyl segments. Sci Hortic 23: 159–162

    Google Scholar 

  5. Einset JW (1978) Citrus tissue culture. Stimulation of fruit explant cultures with orange juice. Plant Physiol 62: 885–888

    Google Scholar 

  6. Frost HB, Krug CA (1942) Diploid-tetraploid periclinal chimeras as bud variants in citrus. Genetics 27: 619–634

    Google Scholar 

  7. Hidaka T (1984) Induction of plantlet from anthers of ‘Trovita’ orange (Citrus sinensis Osbeck). J Jpn Soc Hortic Sci 53: 1–5

    Google Scholar 

  8. Hidaka T, Kajiura I (1988) Plantlet differentiation from callus protoplasts induced from Citrus embryo. Sci Hortic 34: 85–92

    Google Scholar 

  9. Hidaka T, Yamada Y, Shichijo T (1982) Plantlet formation by anther culture of Citrus aurantium L. Jpn J Breed 32: 247–252

    Google Scholar 

  10. Iwamasa M, Nishiura M (1970) Evidence for the chimeral nature of vegetative reversion in Suzuki Wase, an early ripening satsuma mandarin. Sabrao Newslett 2: 109–114

    Google Scholar 

  11. Kato Y (1980) Studies on juice vesicles isolated from mature and immature citrus fruit. I. Callus induction and cell differentiation in Citrus hassaku Hort. ex Tanaka. J Jpn Soc Hortic Sci 49: 36–40

    Google Scholar 

  12. Kobayashi S, Ikeda I, Nakatani I (1984) Induction of nucellar callus from orange (Citrus sinensis Osb.) ovules, and uniformity of regenerated plants. Bull Fruit Tree Res Stn Series E (Akitsu) 5: 43–54

    Google Scholar 

  13. Kobayashi S, Ohgawara T, Ohgawara E, Oiyama I, Ishii S (1988) A somatic hybrid plant obtained by protoplast fusion between navel orange (Citrus sinensis) and satsuma mandarin (C. unshiu). Plant Cell Tissue Organ Culture 14: 63–69

    Google Scholar 

  14. Kobayashi S, Uchimiya H, Ikeda I (1983) Plant regeneration from ‘Trovita’ orange protoplasts. Jpn J Breed 33: 119–122

    Google Scholar 

  15. Kochba J, Spiegel-Roy P, Safran H (1972) Adventive plants from ovules and nucelli in Citrus. Planta 106: 237–245

    Google Scholar 

  16. Kordan HA (1963) Growth characteristics of citrus fruit tissue in vitro. Nature 4883: 867–869

    Google Scholar 

  17. Kulshrestha VK, Chauhan YS, Roberts LW (1982) In vitro studies on xylogenesis in Citrus fruit vesicles. I. Effects of auxin, cytokinin and citric acid on induction of xylem differentiation. Phytomorphology 32: 14–17

    Google Scholar 

  18. Ling JT, Nito N, Iwamasa M (1989) Plant regeneration from protoplast of Calamondin (Citrus madurensis Lour.) Sci Hortic 40: 325–333

    Google Scholar 

  19. Machida H, Ooishi A, Hosoi T, Kawai H (1974) Clonal multiplication of citrus through tissue culture. Rep Fac Agric Shizuoka Univ 24: 15–21

    Google Scholar 

  20. Murashige T, Nakano R, Tucker DPH (1967) Histogenesis and rate of nuclear change in Citrus limon tissue in vitro. Phytomorphology 17: 469–476

    Google Scholar 

  21. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15: 473–497

    Google Scholar 

  22. Murashige T, Tucker DPH (1969) Growth factor requirements of citrus tissue culture. Proc 1st Int Citrus Symp 3: 1155–1161

    Google Scholar 

  23. Ohgawara T, Kobayashi S, Ohgawara E, Uchimiya H, Ishii S (1985) Somatic hybrid plants obtained by protoplast fusion between Citrus sinensis and Poncirus trifoliata. Theor Appl Genet 71: 1–4

    Google Scholar 

  24. Unger JW, Feng KA (1978) Growth and differentiation of juice vesicles of orange grown in vitro. Am J Bot 65: 511–515

    Google Scholar 

  25. Vardi A (1981) Protoplast derived plants from different citrus species and cultivars. Proc Int Soc Citriculture 1981 1: 149–152

    Google Scholar 

  26. Vardi A, Breiman A, Glun E (1987) Citrus cybrids: production by donor-recipient protoplast-fusion and verification by mitochondrial-DNA restriction profiles. Theor Appl Genet 75: 51–58

    Google Scholar 

  27. Vardi A, Spiegel-Roy P, Glun E (1975) Citrus cell culture: isolation of protoplasts, plating densities, effect of mutagens and regeneration of embryoids. Plant Sci Lett 4: 231–236

    Google Scholar 

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Nito, N., Iwamasa, M. In vitro plantlet formation from juice vesicle callus of satsuma (Citrus unshiu Marc.). Plant Cell Tiss Organ Cult 20, 137–140 (1990). https://doi.org/10.1007/BF00114712

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  • DOI: https://doi.org/10.1007/BF00114712

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