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Cytochrome P-450-dependent catabolism of triethanolamine in Rhodotorula mucilaginosa

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Abstract

The yeast Rhodotorula mucilaginosa was able to grow in media containing triethanolamine or diethanolamine as the sole nitrogen source. During growth in the presence of triethanolamine, extracts of yeast cells contained increased levels of cytochrome P-450 dependent monooxygenase which catalyzed the oxidative N-dealkylation of aminoalcohols. Formation of diethanolamine, ethanolamine and glyoxylate from triethanolamine was demonstrated, and the identity of the products was verified by thin layer chromatography. These observations suggested the following scheme of triethanolamine catabolism: triethanolamine → diethanolamine + glycolaldehyde, diethanolamine → ethanolamine + glycolaldehyde, ethanolamine → NH3 + glycolaldehyde → glycolate → glyoxylate → glycerate pathway.

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References

  1. Bennet J (1986) Some effects of trishydroxyaminomethane (TRIS') on the activity of urease. Biochem. Soc. Trans. 14: 365

    Google Scholar 

  2. Blatiak A, King DJ & Wiseman A (1985) Effects of alcohols on the level of cytochrome P-450 in Saccharomyces cerevisiae after resuspension in buffer. Biochem. Soc. Trans. 13: 924

    Google Scholar 

  3. Bonfanti M, Airoldi L & Fanelli R (1984) Identification of a nitrosamino aldehyde and nitrosamino acid resulting from β-oxidation of N-nitrosodiethanolamines. Chem. Biol. Interact. 51: 103–113

    Google Scholar 

  4. Dixon GH & Kornberg HL (1959) Assay methods for the key enzymes of the glyoxylate cycle. Biochem. J. 72: 195–198

    Google Scholar 

  5. Fattakhova AN, Ofitserov EN, Diyakov VM & Naumova RP (1987) Utilization of 1-Chloromethylsilatrane by Rhodotorula mucilaginosa. FEMS Microbiol. Lett. 48: 317–319

    Google Scholar 

  6. Garnon AE, Adams MC & Bennett ED (1978) Microbial degradation of diethanolamine and related compounds. Microbios 23: 7–18

    Google Scholar 

  7. Green J & Large PJ (1983) Oxidation of dimethylamine and trimethylamine in methazotrophic yeasts by microsomal mono-oxygenases sensitive to carbon monoxide. Biochim. Biophys. Res. Commun. 113: 900–907

    Google Scholar 

  8. Haywood CW & Large PJ (1981) Microbial oxidation of amines. Biochem. J. 199: 187–201

    Google Scholar 

  9. Karenlampi SO & Mikkila PH (1981) Polymer phase partition in the purification of cytochrome P-450 and cytochrome b from the yeast Brettanomyces anomalus. Biotechnol. Appl. Biochem. 8: 60–68

    Google Scholar 

  10. Krauzova VT & Sharyshev AA (1987) Subcellular distribution of enzymes of N-alkane oxidation primary steps in the yeast Candida maltosa. Biochimiya 52: 599–606 (in Russian)

    Google Scholar 

  11. Loeppky RH, Hansen TJ & Kufer LK (1983) Reducing nitrosamine contamination in cutting fluids. Food Chem. Toxicol. 21: 607–613

    Google Scholar 

  12. Lord JM (1972) Glycolate oxidoreductase in Escherichia coli. Biochim. Biophys. Acta 267: 227–237

    Google Scholar 

  13. Lowry OH, Rosenbrough MJ, Fars SL & Randall RJ (1951) Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265–275

    Google Scholar 

  14. Lurye UU (1984) Analytical chemistry of industrial sewage (pp 174–191). Chimiya, Moscow (in Russian)

    Google Scholar 

  15. Omura T & Sato R (1964) The carbon monoxide-binding pigment of liver microsomes. J. Biol. Chem. 239: 2370–2378

    Google Scholar 

  16. Ornston LH & Ornston MK (1969) Regulation of glyoxylate metabolism in Escherichia coli K-12. J. Bacteriol. 98: 1098–1108

    Google Scholar 

  17. Quayle JR & Taylor GA (1961) Carbon assimilation by Pseudomonas oxalatus (OX 1). Biochem. J. 78: 611–615

    Google Scholar 

  18. Sadler AM, Winkler MA & Wiseman A (1985) Recovery of microbial cytochrome P-450 from yeast using the low speed centrifugation. Chem Engin. J. 30: B43-B49

    Google Scholar 

  19. Sanglard D, Kappeli O & Fiechter A (1984) Metabolic conditions determining the composition and catalytic activity of cytochrome P-450 monooxygenases in Candida tropicalis. J. Bacteriol. 157: 297–302

    Google Scholar 

  20. Shoun H, Suyma M & Yasui T (1989) Soluble, nitrate/nitrite-inducible cytochrome P-450 of the fungus, Fusarium oxysporum. FEBS Lett. 244: 11–14

    Google Scholar 

  21. Voronkov MG, Kuznetsov JG & Diyakov VM (1982) New biostimulator mival in farming (pp 10–89). Nauka, Moscow (in Russian)

    Google Scholar 

  22. Walker JPL & Taylor BG (1983) Metabolism of floroglucinol by Fusarium solani. Arch. Microbiol. 134: 123–126

    Google Scholar 

  23. Whitefield D & Large P (1986) Enzymes metabolizing dimethylamine, trimethylamine and trimethylamine-N-oxide in the yeast Sporopachydermia cereana grown on amines as sole nitrogen source. FEMS Microbiol. Lett. 35: 99–105

    Google Scholar 

  24. Williams GR & Callely A (1982) The biodegradation of diethanolamine and triethanolamine by a yellow Gramnegative rod. J. Gen. Microbiol. 128: 1203–1209

    Google Scholar 

  25. Zwart KB & Harder W (1983) Regulation of the metabolism of some alkylated amines in the yeasts Candida utilis and Hansenula polymorpha. J. Gen. Microbiol. 129: 3157–3168

    Google Scholar 

  26. Zwart KB, Veenhuis M & Harder W (1983) Significance of yeast peroxisomes in the metabolism of choline and ethanolamine. Antonie van Leeuwenhoek 49: 369–385

    Google Scholar 

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Authors and Affiliations

  1. The Kazan State University, 420008, Kazan, USSR

    A. N. Fattakhova, E. N. Ofitserov & A. V. Garusov

Authors
  1. A. N. Fattakhova
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  2. E. N. Ofitserov
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  3. A. V. Garusov
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Fattakhova, A.N., Ofitserov, E.N. & Garusov, A.V. Cytochrome P-450-dependent catabolism of triethanolamine in Rhodotorula mucilaginosa . Biodegradation 2, 107–113 (1991). https://doi.org/10.1007/BF00114600

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  • DOI: https://doi.org/10.1007/BF00114600

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