Two widely used B16 melanoma cell lines of low and high lung colonizing potential (B16-F1 and B16-F10) were compared in their ability to induce platelet aggregation. The results of these experiments showed a reproducible difference in platelet aggregating activity of these two cell lines which directly correlated with their lung colonizing potentials. However, when clones were derived from these heterogeneous cell lines and tested for experimental metastatic potential, platelet aggregating ability and Met-72 expression, no correlation could be attached to the platelet aggregating activity of the clones. Results of these experiments provide direct evidence that platelet aggregation is not an accurate index of experimental metastatic potential of tumor cell clones, nor is it an essential trait of all metastatic cells. The ability of tumor cells to induce platelet aggregation is examined and discussed in the context of cellular heterogeneity.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
AL-Mondhiry, H., 1984, Tumor interaction with hemostasis: the rationale for the use of platelet inhibitors and anticoagulants in the treatment of cancer. American Journal of Hematology, 16, 193–202.
Chew, E. C., and Wallace, A. A., 1976, Demonstration of fibrin in early stages of experimental metastasis. Cancer Research, 36, 1904–1909.
Estrada, J., and Nicolson, G. L., 1984, Tumor cell platelet aggregation does not correlate with metastatic potential of rat 13762NF mammary adenocarcinoma tumor cell clones. International Journal of Cancer, 34, 101–105.
Fidler, I. J., 1973, Selection of successive tumor lines for metastasis. Nature New Biology, 242, 148–149.
Fidler, I. J., Gersten, D. M., and Hart, I. R., 1978, The biology of cancer invasion and metastasis. Advances in Cancer Research, 28, 149–250.
Gasic, G. J., Gasic, T. B., Galanti, N., Johnson, T., and Murphy, S., 1973, Platelet-tumor cell interactions in mice. The role of platelets in the spread of malignant disease. International Journal of Cancer, 11, 704–718.
Gasic, G. L., Gasic, T. B., and Stewart, C. C., 1968, Antimetastatic effects associated with platelet reduction. Proceedings of the National Academy of Sciences, USA, 61, 46–52.
Hilgard, P., 1974, The role of blood platelets in experimental metastasis. British Journal of Cancer, 28, 429–435.
Hilgard, P., and Thornes, R. D., 1976, Anticoagulants in the treatment of cancer. European Journal of Cancer, 12, 755–762.
Karpatkin, S., and Pearlstein, E., 1981, Role of platelets in tumor cell metastases. Annals of International Medicine, 95, 636–641.
Kimura, A. K., and Xiang, J., 1986, High levels of Met-72 antigen expression: correlation with metastatic activity of B16 melanoma tumor cell variants. Journal of the National Cancer Institute, 76, 1247–1254.
Liotta, L. A., Kleinerman, J., and Saidel, G. M., 1976, The significance of hematogenous tumor cell clumps in the metastatic process. Cancer Research, 36, 889–894.
Mehta, P., Kimura, A. K., and Gee, A., 1984, Platelet-tumor cell interaction in relation to route of metastatic spread of tumor cell lines. Blood, 64, 249 (abstract).
Nicolson, G. L., 1982, Cancer metastasis: organ colonization and cell surface properties of malignant cells. Biochimica et Biophysica Acta, 695, 113–176.
Nicolson, G. L., and Poste, G., 1982, Tumor cell diversity and host responses in cancer metastasis. 1. Properties of metastatic cells. Current Problems in Cancer, 7, 4–83.
Pearlstein, E., Salk, P. L., Yogeeswaran, G., and Karpatkin, S., 1980, Correlation between spontaneous metastatic potential, platelet-aggregating activity of cell surface extracts, and cell surface sialylation in 10 metastatic-variant derivatives of a rat renal sarcoma cell line. Proceedings of the National Academy of Sciences, USA, 293, 4336–4339.
Roos, E., and Dingemans, K. P., 1979, Mechanisms of metastasis. Biochimica et Biophysica Acta, 560, 135–166.
Tsuruo, T., Kawabata, H., Iida, H., and Yamori, T., 1986, Tumor-induced platelet aggregation and growth promoting factors as determinants for successful tumor metastasis. Clinical and Experimental Metastasis, 4, 25–33.
Warren, B. A., 1981, The origin and fate of blood-borne tumor emboli. Cancer Biology Reviews, 2, 95–169.
Xiang, J., and Kimura, A. K., 1986, In vitro modulation of the metastatic phenotype. I. Analysis of differentiation forms of the B16 melanoma expressing Met-72 determinants and metastatic activity. Clinical and Experimental Metastasis, 4, 293–309.
Xiang, J., and Kimura, A. K., 1987, Isolation of metastatic B16 melanoma variants using anti-Met-72 monoclonal antibodies and flow cytometry. Clinical and Experimental Metastasis, 5, 35–42.
Author information
Authors and Affiliations
Additional information
This work was supported in part by U.S. Public Health Service Grants CA 40351 and CA 09126 from the National Cancer Institute, DHHS.
Rights and permissions
About this article
Cite this article
Kimura, A.K., Mehta, P., Xiang, J. et al. The lack of correlation between experimental metastatic potential and platelet aggregating activity of B16 melanoma clones viewed in relation to tumor cell heterogeneity. Clin Exp Metast 5, 125–133 (1987). https://doi.org/10.1007/BF00058058
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00058058