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Diagnostic DNA amplification from individual tick eggs, larvae and nymphs

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Abstract

The species of single tick eggs, larvae and nymphs was determined by PCR amplification and characterization of the hypervariable, second transcribed spacer (ITS2) of the multicopy ribosomal RNA gene (rDNA). Engorgement of larvae and nymphs did not preclude species identification. The method is generally applicable for ixodid and argasid ticks and can be used for epidemiological studies requiring the identification of individuals from pre-adult stages.

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References

  • Black, W.C. and Piesman, J. 1994. Phylogeny of hard-and soft-tick taxa (Acari: Ixodida) based on mitochondrial 16S rDNA sequences. Proc. Natl Acad. Sci. USA. 91: 10034–10038.

    Google Scholar 

  • Hillis, D.M. and Dixon, M.T. 1991. Ribosomal DNA: molecular evolution and phylogenetic inference. Q. Rev. Biol. 66: 411–453.

    Google Scholar 

  • Hiss, R.H., Norris, D.E., Dietrich, C.H., Whitcomb, R.F., West, D.F., Bosio, C.F., Kambhampati, S., Piesman, J., Antolin, M.F. and Black, W.C. 1994. Molecular taxonomy using single-strand conformation polymorphism (SSCP) analysis of mitochondrial ribosomal DNA genes. Insect Mol. Biol. 3: 171–182.

    Google Scholar 

  • McLain, D.K., Wesson, D.M., Oliver, J.H. and Collins, F.H. 1995. Variation in ribosomal DNA internal transcribed spacers 1 among eastern populations of Ixodes scapularis (Acari: Ixodidae). J. Med. Entomol. 32: 353–360.

    Google Scholar 

  • Seetharam, S. and Dicker, I.B. 1991. A rapid and complete 4-step protocol for obtaining nucleotide sequence from E. coli genomic DNA from overnight cultures. Biotechniques 11: 32–33.

    Google Scholar 

  • Wesson, D.M., McLain, D.K., Oliver, J.H., Piesman, J. and Collins, F.H. 1993. Investigation of the validity of species status of Ixodes dammini (Acari: Ixodidae) using rDNA. Proc. Natl Acad. Sci. USA. 90: 10221–10225.

    Google Scholar 

  • Zahler, M., Gothe, R. and Rinder, H. 1995. Genetic evidence against a morphologically suggestive conspecificity of Dermacentor reticulatus and D. marginatus (Acari: Ixodidae). Int. J. Parasitol. in press.

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Authors and Affiliations

  1. Institute for Comparative Tropical Medicine and Parasitology, University of Munich, Leopoldstr. 5, 80802, Munich, Germany

    Monika Zahler & Rainer Gothe

  2. Department of Infectious Diseases and Tropical Medicine, University of Munich, Leopoldstr. 5, 80802, Munich, Germany

    Heinz Rinder

Authors
  1. Monika Zahler
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  2. Rainer Gothe
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  3. Heinz Rinder
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Zahler, M., Gothe, R. & Rinder, H. Diagnostic DNA amplification from individual tick eggs, larvae and nymphs. Exp Appl Acarol 19, 731–736 (1995). https://doi.org/10.1007/BF00052084

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  • DOI: https://doi.org/10.1007/BF00052084

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