Abstract
The effective molecular weight cut-off values of dialysis membranes for carrageenan and alginate oligosaccharides were evaluated by gel permeation chromatography and nuclear magnetic resonance spectroscopy. For the different membranes tested, i.e. Medicell, Spectra Por 1000D and 3500D, the porous sizes are analogous to tri- and tetrasaccharides. A simple dialysis can be used to recover the majority of the oligosaccharides produced by a carrageenase or an alginate lyase digestion.
Similar content being viewed by others
References
Arnott, S. & W. E. Scott, 1972. Accurate X-ray diffraction analysis of fibrous polysaccharides containing pyranose rings. Part I. The linked atom approach. J. Chem. Soc. Perkin Trans. II: 324–335.
Cantor, C. S. & P. R. Schimmel, 1980. Biophysical chemistry Part II. Techniques for the study of biological structure and function. W. H. Freeman, New York, 655 pp.
Dubois, M., K. Gilles, A. J. K. Hamilton, P. A. Rebers & F. Smith, 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350–356.
Knutsen, S. H. & H. Grasdalen, 1992a. Analysis of carrageenans by enzymic degradation, gel filtration and 1H-nmr. Carbohydr. Polym. (in press).
Knutsen, S. H. & H. Grasdalen, 1992b. The use of neocarrabiose oligosaccharides with different length and sulphate substitution as model compounds for 1H-nmr spectroscopy. Carbohydr. Res. 229: 233–244.
Larsen, B., O. Smidsrød, A. Haug & T. Painter, 1969. Determination by a kinetic method of the nearest neighbour frequencies in a fragment of alginic acid. Acta Chem. Scand. 23: 2375–2388.
McLean, M. W. & F. B. Williamson, 1979. Kappa-carrageenan from Pseudomonas carrageenovora. Eur. J. Biochem. 93: 553–558.
Piculell, L. & R. Rymden, 1989. The helix-coil transition on an ionic polysaccharide probed by counterion self-diffusion measurements. Macromolecules 22: 2376–2380.
Rochas, C., D. Domard & M. Rinaudo, 1980. Aqueous GPC of electrolytes and polyelectrolytes. Eur. Polym. J. 16: 135–140.
Rochas, C. & A. Heyraud, 1982. Acid and enzymic hydrolysis of kappa-carrageenan. Polymer Bull. 5: 81–86.
Skjåk-Bræk, G., B. Larsen & H. Grasdalen, 1985. The role of O-acetyl groups in the biosynthesis of alginate by Azotobacter vinelandii. Carbohydr. Res. 145: 169–174.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Knutsen, S.H., Moe, S.T., Larsen, B. et al. Molecular cut-off values of dialysis membranes for alginate and kappa-carrageenan oligosaccharides. Hydrobiologia 260, 667–672 (1993). https://doi.org/10.1007/BF00049086
Issue Date:
DOI: https://doi.org/10.1007/BF00049086