Skip to main content
SpringerLink
Log in

Kinetics and specificity of alginate lyases

  • Published:
Hydrobiologia Aims and scope Submit manuscript

Abstract

A purified preparation of the extracellular alginate lyase has been used to study kinetics and specificity towards purified, homopolymeric fragments of alginate. The enzyme preparation from Bacillus circulans 1351 degraded both block types, although with different efficiency, and thus appears to be nonspecific. Addition of calcium ions markedly enhanced the reaction rate for the polymannuronate block but had little or no effect on the reaction with polyguluronate. Michaelis-Menten kinetics are not obeyed in the absence of calcium ions and only for the polymannuronate in the presence of calcium

The study of progress curves in response to variation in substrate and enzyme concentrations strongly suggests that the abalone lyase is subject to a reversible product inhibition.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Boyen, C., B. Kloareg, M. Polne-Fuller & A. Gibor, 1990. Preparation of alginate lyases from marine molluscs for protoplast isolation in brown algae. Phycologia 29: 173–181.

    Google Scholar 

  • Grasdalen, H., B. Larsen & O. Smidsrod, 1979. A P.M.R. study of the composition and sequence of uronate residues in alginate. Carbohydr. Res. 68: 23–31.

    Google Scholar 

  • Hansen, J. B. & L. K. Nakamura, 1985. Distribution of alginate lyase activity among strains of Bacillus circulans. Appl. envir. Microbiol. 49: 1019–21.

    Google Scholar 

  • Haug, A., B. Larsen & E. Bardseth, 1969. Comparison of the constitution of alginates from different sources. Proc. Int. Seaweed Symp. 6: 443–451.

    Google Scholar 

  • Haug, A., B. Larsen & O. Smidsrod, 1974. Uronic acid sequence in alginates from different sources. Carbohydr. Res. 32: 217–225.

    Google Scholar 

  • Haugen, F., F. Kortner & B. Larsen, 1990. Kinetics and specificity of alginate lyases: Part 1; A case study. Carbohydr. Res. 198: 101–109.

    Google Scholar 

  • Østgaard, K., 1993. Determination of alginate composition by a simple enzymatic assay. In A. R. O. Chapman, M. T. Brown & M. Lahaye(eds), Fourteenth International Seaweed Symposium. Developments in Hydrobiology 85. Kluwer Academic Publishers, Dordrecht: 513–520. Reprinted from Hydrobiology 260/261.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Biotechnology, University of Trondheim, N-7034, Trondheim-NTH, Norway

    Bjørn Larsen, Kirsti Hoøen & Kjetill Østgaard

Authors
  1. Bjørn Larsen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kirsti Hoøen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kjetill Østgaard
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Larsen, B., Hoøen, K. & Østgaard, K. Kinetics and specificity of alginate lyases. Hydrobiologia 260, 557–561 (1993). https://doi.org/10.1007/BF00049070

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00049070

Key words

Search

Navigation