Abstract
Callus-like structures (CLS) were observed to develop from sectioned tissues of the thallus segments of Grateloupia filiformis Kützing maintained in Von Stosch enriched-seawater culture medium. Three types were observed: dark CLS originated from pigmented cortical cells, clear CLS and loose clear CLS both originated from colorless medullary cells. Dark and clear CLS developed from segments exposed to light while loose clear CLS developed in darkness. Frequencies of different types of CLS varied according to the origin of the segments (apical, median and basal zones of primary branches). In order to assess the potential for plant regeneration, all CLS were isolated from the original segment and cultured under light. The potential for plant regeneration depends on the type of CLS and on the origin of the segments. The loose clear CLS and the CLS from apical segments produced the largest number of regenerated plantlets. Cultures of CLS might be an effective system for micropropagation in G. filiformis and light seems to be an important factor involved in mechanisms of plant regeneration.
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Yokoya, N.S., Guimarães, S.M.P.B. & Handro, W. Development of callus-like structures and plant regeneration in thallus segments of Grateloupia filiformis Kützing (Rhodophyta). Hydrobiologia 260, 407–413 (1993). https://doi.org/10.1007/BF00049049
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DOI: https://doi.org/10.1007/BF00049049