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A quantitative description of fluorescence excitation spectra in intact bean leaves greened under intermittent light

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Abstract

We present a simple approach for the calculation of in vivo fluorescence excitation spectra from measured absorbance spectra of the isolated pigments involved. Taking into account shading of the pigments by each other, energy transfer from carotene to chlorophyll a, and light scattering by the leaf tissue, we arrive at a model function with 6 free parameters. Fitting them to the measured fluorescence excitation spectrum yields good correspondence between theory and experiment, and parameter estimates which agree with independent measurements. The results are discussed with respect to the origin and the interpretation of in vivo excitation spectra in general.

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Abbreviations

a:

absorptance (=1-transmittance)

A:

absorbance (=-log transmittance)

Chl:

chlorophyll

PS:

photosystem

λ:

wavelength

References

  • Akoyunoglou G (1981) Assembly of functional components in chloroplast photosynthetic membranes. In: Akoyunoglou G (ed) Photosynthesis, Chloroplast Development, Vol 5, pp 353–366. Philadelphia: Balaban

    Google Scholar 

  • Akoyunoglou G, Argyroudi-Akoyunoglou JH, Michel-Wolwertz MR and Sironval C (1966) Effect of intermittent and continuous light on chlorophyll formation in etiolated plants. Physiol Plant 19: 1101–1104

    Google Scholar 

  • Akoyunoglou G and Michelinaki-Maneta M (1975) Development of photosynthetic activity in flashed bean leaves. In: Avron M (ed) Proceedings of the 3rd International Congress on Photosynthesis Research, Vol 3, pp 1885–1896. Amsterdam: Elsevier

    Google Scholar 

  • Brody SS (1988) The position of the carotene in the D-1/D-2 sub-core complex of photosystem II. Z Naturforsch 43c: 226–230

    Google Scholar 

  • Butler WL (1964) Absorption spectroscopy in vivo: Theory and application. Ann Rev Plant Physiol 15: 451–470

    Google Scholar 

  • Butler WL (1965) Development of photosynthetic systems 1 and 2 in a greening leaf. Biochim Biophys Acta 102: 1–8

    Google Scholar 

  • Butler WL (1972) Absorption spectroscopy of biological material. Meth Enzymol 24: 3–25

    Google Scholar 

  • Dallinger RF, Woodruff WH and Rodgers MAJ (1981) The lifetime of the excited singlet state of β-carotene: Consequences to photosynthetic light harvesting. Photochem Photobiol 33: 275–277

    Google Scholar 

  • Egger K (1962) Dünnschichtchromatographie der Chloroplastenpigmente. Planta 58: 664–667

    Google Scholar 

  • Goedheer JC (1969) Energy transfer from carotenoids to chlorophyll in blue-green, red and green algae and greening bean leaves. Biochim Biophys Acta 172: 252–265

    Google Scholar 

  • Gugliemelli LA, Dutton HJ, Jursinic PA and Siegelman HW (1981) Energy transfer in a light-harvesting carotenoid-chlorophyll c-chlorophyll a-protein of Phaeodactylum tricornutum. Photochem Photobiol 33: 903–907

    Google Scholar 

  • Hager A und Meyer-Bertenrath T (1966) Die Isolierung und quantitative Bestimmung der Carotinoide und Chlorophylle von Blättern, Algen und isolierten Chloroplasten mit Hilfe dünnschichtchromatographischer Methoden. Planta 69: 198–217

    Google Scholar 

  • Ji TH, Hess JL and Benson AA (1968) Studies on chloroplast membrane structure. I. Association of pigments with chloroplast lamellar protein. Biochim Biophys Acta 150: 676–685

    Google Scholar 

  • Kramer HJM, Amesz J and Rijgersberg CP (1981) Excitation spectra of chlorophyll fluorescence in spinach and barley chloroplasts at 4 K. Biochim Biophys Acta 637: 272–277

    Google Scholar 

  • Mackinney G (1940) Criteria for purity of chlorophyll preparations. J Biol Chem 132: 91–109

    Google Scholar 

  • Meck E and Strasser RJ (1984) Zeaxanthin in a fraction with an excess of lutein. In: Sybesma C (ed) Advances in Photosynthesis Research, Vol 1, pp 717–720. The Hague: Nijhoff/Junk

    Google Scholar 

  • Pfündel E and Strasser RJ (1988) Violaxanthin de-epoxidase in etiolated leaves. Photosynth Res 15: 67–73

    Google Scholar 

  • Pfündel E (1989) Untersuchungen zur Funktion des Violaxanthinzyklus in höheren Pflanzen. Dissertation. University of Stuttgart

  • Plumley FG and Schmidt GW (1987) Reconstitution of chlorophyll a/b light-harvesting complexes: Xanthophyll-dependent assembly and energy transfer. Proc Natl Acad Sci USA 84: 146–150

    Google Scholar 

  • Schäfer E, Fukshansky L and Shropshire W (1983) Action spectroscopy of photoreversible pigment systems. In: Shropshire W and Mohr H (eds) Encyclopedia of Plant Physiology. Photomorphogenesis, Vol 16A, pp 39–68. Belin: Springer

    Google Scholar 

  • Seely GR and Connolly JS (1986) Fluorescence of photosynthetic pigments in vitro. In: Govindjee, Amesz J and Fork DC (eds) Light Emission by Plants and Bacteria, pp 99–133. London: Academic Press

    Google Scholar 

  • Siefermann-Harms D (1985) Carotenoids in photosynthesis. I. Location in photosynthetic membranes and light-harvesting function. Biochim Biophys Acta 811: 325–355

    Google Scholar 

  • Siefermann-Harms D (1987) The light-harvesting and protective functions of carotenoids in photosynthetic membranes. Physiol Plant 69: 561–568

    Google Scholar 

  • Siefermann-Harms D Michel J-M and Collard F (1980) Carotenoid transformation underlying the blue absorbance evolution. Biochim Biophys Acta 589: 315–323

    Google Scholar 

  • Siefermann-Harms D and Ninnemann H (1982) Pigment organization in the light-harvesting chlorophyll-a/b protein complex of lettuce chloroplasts. Evidence obtained from protection of the chlorophylls against proton attack and from excitation energy transfer. Photochem Photobiol 35: 719–731

    Google Scholar 

  • Smillie RM, Nielsen NC, Henningsen KW and Von Wettstein D (1975) Ontogeny and environmental regulation of photochemical activity in chloroplast membranes. In: Avron M (ed) Proceedings of the 3rd International Congress on Photosynthesis Research, Vol 3, pp 1841–1860. Amsterdam: Elsevier

    Google Scholar 

  • Stahl U, Tusov VB, Paschenko VZ and Voigt J (1989) Spectroscopic investigations of fluorescence behavior, role and function of the long-wavelength pigments of photosystem I. Biochim Biophys Acta 973: 198–204 198–204

    Google Scholar 

  • Strasser RJ and Butler WL (1976) Correlation of absorbance changes and thylakoid fusion with the induction of oxygen evolution in bean leaves greened by brief flashes. Plant Physiol 58: 371–376

    Google Scholar 

  • Strasser RJ and Butler WL (1977) The yield of energy transfer a the spectral distribution of excitation energy in the photochemical apparatus of flashed bean leaves. Biochim Biophys Acta 462: 295–306

    Google Scholar 

  • Vogelmann TC (1989) Penetration of light into plants. Photochem Photobiol 50: 895–902

    Google Scholar 

  • Wróbel D and Hendrich W (1989) Thermal deactivation and energy transfer in isolated photosystem 2 and light-harvesting complexes in poly(vinyl alcohol) film. J Photochem Photobiol 3: 319–332

    Google Scholar 

  • Ziegler R und Egle K (1965) Zur quantitativen Analyse der Chloroplastenpigmente. I. Kritische Überprüfung der spektralphotometrischen Chlorophyll-Bestimmung. Beitr Biol Pflanzen 41: 11–37

    Google Scholar 

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Author notes

  1. Erhard Pfündel

    Present address: Dept. of Biological Sciences, Purdue University, Lilly Hall of Life Sciences, 47907, West Lafayette, IN, USA

  2. Ellen Baake

    Present address: Institute of Mathematics, Universitätsstr. 2, D-8900, Augsburg, FRG

Authors and Affiliations

  1. Institute of Biology, Dept. of Bioenergetics, University of Stuttgart, Pfaffenwaldring 57, D-7000, Stuttgart 80, FRG

    Erhard Pfündel

  2. Dept. of Theoretical Biology, University of Bonn, Kirschallee 1, D-5300, Bonn 1, FRG

    Ellen Baake

Authors
  1. Erhard Pfündel
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  2. Ellen Baake
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Pfündel, E., Baake, E. A quantitative description of fluorescence excitation spectra in intact bean leaves greened under intermittent light. Photosynth Res 26, 19–28 (1990). https://doi.org/10.1007/BF00048973

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  • DOI: https://doi.org/10.1007/BF00048973

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