Abstract
The present study was conducted to determine whether Varroa jacobsoni can transmit American foulbrood (AFB), caused by the bacterium Paenibacillus larvae to healthy colonies by the surface transport of spores. Five two-storey Langstroth colonies of Apis mellifera ligustica were infested by placing a sealed brood comb, with 10% Varroa prevalence, between the central brood combs of each colony. Two months later the colonies were inoculated with P. larvae by adding brood comb pieces with clinical signs of AFB (45±5 scales per colony). After 60 days the brood area was completely uncapped by means of dissecting needles and tweezers, separating the Varroa mites from the larvae and the collected mites were introduced at a rate of 51 per colony into four recipient hives placed in an isolated apiary. Twenty female Varroa specimens were separated at random and observed by SEM. Paenibacillus larvae spores were found on the dorsal shield surface and on idiosomal setae. All colonies died after 4–5 months due to a high incidence of varroosis. No clinical AFB symptoms or P. larvae spores were observed in microscopic preparations. It is concluded that Varroa jacobsoni does not transmit AFB from infected to healthy colonies; it does, however transport P. larvae spores on its surface.
References
Alippi A. M. 1991. A comparison of laboratory techniques for the detection of significant bacteria of the honey bee, Apis mellifera, in Argentina. J. Apicult. Res. 30: 75–80.
Alippi A. M. 1992. Transporte de esporas de Bacillus larvae por el ácaro Varroa jacobsoni. Rev. Facult. de Agronomi. de La Plata 68: 83–86.
Ash C., Priest F.G. and Collins M.D. 1993. Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus. Antonie van Leeuwenhoek 64: 253–260.
Bailey L. 1991. Honey Bee Pathology, 2nd edn. Academic Press, London, New York.
Ball B. V. 1985. Acute paralysis virus isolates from honey bee colonies infested with Varroa jacobsoni. J. Apicult. Res 24: 115–119
Ball B. V. 1988. The impact of secondary infections in honey-bee colonies infested with the parasitic mite Varroa jacobsoni. In Africanized honey bees and bee mites, Needham J.G. (ed.), Ellis Horwood, Cheshire. pp. 457–461.
Ball B.V. and Allen M. F. 1988. The prevalence of pathogens in honey bee (Apis mellifera) colonies infested with the parasitic mite Varroa jacobsoni. Ann. Appl. Biol. 113: 237–224.
Glinski Z. F. and Jarosz J. 1990a. Serratia marcescens, artificially contaminating brood and worker honeybees, contaminates the Varroa jacobsoni mite. J. Apicult. Res. 29: 107–111.
Glinski Z. F. and Jarosz J. 1990b. Micro-organisms associated fortuitously with Varroa jacobsoni. Microbios 62: 59–68.
Glinski Z. F. and Jarosz J. 1992. Varroa jacobsoni as a carrier of bacterial infections to a recipient bee host. Apidologie 23: 25–31.
Gochnauer, T. A., Furgala, B. and Shimanuki, H. 1979. Diseases and enemies of the honey bee. In The hive and the honey bee, Dadant & Sons, Hamilton, IL. (eds.) pp. 617–619.
Gordon, R., Haynes, W.C. and Pang, C. H-N. 1973. The genus Bacillus. USDA Handbook 427.
Grobov O.F. 1977. Varroasis de las abejas. In La varroasis, enfermedad de la abeja melífera, Editorial Apimondia, Bucarest. pp. 52–92. Washington, D.C., USA.
Hornitzky M. A. Z. and Clark S. 1991. Culture of Bacillus larvae from bulk honey samples for the detection of American Foulbrood. J. Apicult. Res. 32: 51–52.
Hornitzky M.A.Z. and Karlovskis S. 1989. A culture technique for the detection of Bacillus larvae in honey bees. J. Apicult. Res. 28: 118–120.
Marcangeli J., Monetti L. and Fernandez N. 1992. Malformations produced by Varroa jacobsoni on Apis mellifera in the province of Buenos Aires, Argentina. Apidologies 23: 399–402.
Puerta F., Flores J. M., Jimenez A. J., Bustos M. and Padilla F. 1990. Enfermedades secundarias a la parasitación por varroa en Apis mellifera. Vida Apícola 43: 54–59.
Shimanuki H. and Knox D.A. 1991. Diagnosis of Honey Bee Diseases. US Department of Agriculture, Springfield, VA, USA.
Strick H. and Madel G. 1988. Transmission of the pathogenic bacterium Hafnia alvei to honey bees by the ectoparasitic mite Varroa jacobsoni. In Africanized honey bees and bee mites, Needham J.G. (ed.), pp. 462–466. Ellis Horwood, Cheshire.
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Alippi, A.M., Albo, G.N., Marcangeli, J. et al. The mite Varroa jacobsoni does not transmit American foulbrood from infected to healthy colonies. Exp Appl Acarol 19, 607–613 (1995). https://doi.org/10.1007/BF00048815
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DOI: https://doi.org/10.1007/BF00048815