Abstract
The effectiveness of four phosphinothricin (PPT)-based selective agents were evaluated for use in maize transformation: glufosinate, bialaphos, Basta® and Herbiace®. Glufosinate and its commercial formulation, Basta®, were less effective in controlling growth of non-transgenic corn callus than the tripeptide, bialaphos, or its commercial formulation, Herbiace®. Addition of 25 mM l-proline had no significant effect on selection when using bialaphos. However, when l-proline was included with the selective agent glufosinate, selection was inhibited and callus growth was enhanced. At four weeks, callus growth on 0.3, 1.0 and 3.0 mg l-1 glufosinate in the presence of proline was 76, 43, and 21% of control growth, respectively, and in the absence of proline was only 32, 9, and 6% of control growth. Optimized selection protocols for Basta® and bialaphos yielded comparable numbers of transformants. Using these protocols, fertile transgenic plants were regenerated from transformed callus cultures.
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Abbreviations
- AA:
-
amino acid
- GS:
-
glutamine synthetase
- PAT:
-
phosphinothricin acetyl transferase
- PPT:
-
2-amino-(methylphosphinyl)-butanoic acid (phosphinothricin)
- 2,4-d :
-
2,4-dichlorophenoxyacetic acid
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Dennehey, B.K., Retersen, W.L., Ford-Santino, C. et al. Comparison of selective agents for use with the selectable marker gene bar in maize transformation. Plant Cell Tiss Organ Cult 36, 1–7 (1994). https://doi.org/10.1007/BF00048308
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DOI: https://doi.org/10.1007/BF00048308