Abstract
Axenic transformed shoot cultures of Pimpinella anisum (anise) were established following inoculation of plant stems with the nopaline strain T37 of Agrobacterium tumefaciens. The stable incorporation of T-DNA in the transformed tissues was demonstrated by polymerase chain reaction. Total essential oil accumulated by transformed shoot cultures grown under continuous light was found to be 18% lower (per unit fresh weight of tissue) than that produced by untransformed shoot cultures incubated under similar conditions, but more than 89% lower than the yield of oil from the intact plant. The relative amounts of the principal components of the essential oil of the transformed shoot cultures, namely geraniol, β-bisabolene, trans-pseudoisoeugenol-2-methylbutyrate and transanethole, were similar to those present in the parent plant, but significantly different from those of the untransformed shoot cultures.
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Abbreviations
- T-DNA:
-
transfer-DNA
- MS:
-
Murashige and Skoog medium
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- TY:
-
tryptone and yeast extract medium
- tD :
-
doubling time
- GC-MS:
-
gas chromatography coupled with mass spectrometry
- FID:
-
flame ionisation detector
- PCR:
-
polymerase chain reaction
- TE:
-
Tris-HCl, EDTA buffer
- TBE:
-
Tris, borate, EDTA buffer
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Salem, K.M.S.A., Charlwood, B.V. Accumulation of essential oils by Agrobacterium tumefaciens-transformed shoot cultures of Pimpinella anisum . Plant Cell Tiss Organ Cult 40, 209–215 (1995). https://doi.org/10.1007/BF00048125
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DOI: https://doi.org/10.1007/BF00048125