Skip to main content
SpringerLink
Log in

Nitrogen and phosphorus uptake by the Brazilian kelp Laminaria abyssalis (Phaeophyta) in culture

  • 10. Physiology, biochemistry and cell biology
  • Published:
Hydrobiologia Aims and scope Submit manuscript

Abstract

The uptake of ammonium, nitrate and phosphate by laboratory-grown young sporophytes of Laminaria abyssalis was measured in a perturbed system (batch mode) at 18 °C and 35 ± 5 µE m−2 s−1 photon flux density. Uptake of all appeared to follow saturation-type nutrient uptake kinetics. The NO sup−inf3 (K s = 14.0 µM, V max = 5.0 µmol h−1 g−1 dry wt) and NH sup+inf4 (K s = 4.6 µM, V max= 2.0 µmol h−1 g−1 dry wt) were taken up simultaneously, although NH sup+inf4 was taken up more rapidly. Values of K 3 and V max for phosphate were, respectively, 2.21 µM and 0.83 µmol h−1 g−1 dry wt. Nitrate and phosphate were both consumed in similar rates (V max /Ks ∼ 0.37) at low concentrations. NH sup+inf4 , thus, might be a more efficient form of N fertilizer if artificial enrichment of seawater is used.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Birch, P. B., D. M. Gordon & A. J. McComb, 1981. Nitrogen and phosphorus nutrition of Cladaphora in the Peel-Harvey estuarine system, western Australia. Bot. mar. 24: 381–387.

    Google Scholar 

  • Bird, K. T., 1976. Simultaneous assimilation of ammonium and nitrate by Gelidium nudifrons (Gelidiales: Rhodophyta). J. Phycol. 12: 238–241.

    Google Scholar 

  • Björnsäter, B. R. & P. A. Wheeler, 1990. Effect of nitrogen and phosphorus supply on growth and tissue composition of Ulva fenestrata and Enteromorpha intestinalis (Ulvales, Chlorophyta). J. Phycol. 26: 603–611.

    Google Scholar 

  • Braga, A. C. & Y. Yoneshigue-Valentin, 1994. Growth of Laminaria abyssalis (Phaeophyta) at different nitrate concentrations. Phycologia 33: 271–274.

    Google Scholar 

  • Chapman, D. J. & P. J. Harrison, 1988. Nitrogen metabolism and measurement of nitrate reductase activity. In C. S. Lobban, D. J. Chapman & B. P. Kremer (eds) Experimental Phycology — A Laboratory Manual. Cambridge University Press, Cambridge: 196–202.

    Google Scholar 

  • Darley, W. M., 1982. Algal Biology: a physiological approach. Blackwell Scientific Publications, London, 168 pp.

    Google Scholar 

  • D'Elia, C. R & J. A. DeBoer, 1978. Nutritional studies of two red algae. II. Kinetics of ammonium and nitrate uptake. J. Phycol. 14: 266–272.

    Google Scholar 

  • Dowd, J. E. & D. S. Riggs, 1965. A comparison of estimates of Michaelis-Menten kinetic constants from various linear transformations. J. biol. Chem. 240: 863–869.

    Google Scholar 

  • Druehl, L. D., P. J. Harrison, K. E. Lloyd & P. A. Thompson, 1989. Phenotypic variation in N uptake by Laminaria groenlandica Rosenvinge (Laminariales, Phaeophyta). J. exp. mar. Biol. Ecol. 127: 155–164.

    Google Scholar 

  • Friedlander, M. & C. J. Dawes, 1985. In situ uptake kinetics of ammonium and phosphate and chemical composition of the red seaweed Gracilaria tikvahiae. J. Phycol. 21: 448–453.

    Google Scholar 

  • Gerard, V. A., 1982. In situ rates of nitrate uptake by giant kelp Macrocystis pyrifera (L.) C. Agardh: Tissue differences, environmental effects and predictions of nitrogen-limited growth. J. exp. mar. Biol. Ecol. 62: 211–224.

    Google Scholar 

  • Haines, K. C. & P. A. Wheeler, 1978. Ammonium and nitrate uptake by the marine macrophytes Hypnea musciformis (Rhodophyta) and Macrocystis pyrifera (Phaeophyta). J. Phycol. 14: 319–324.

    Google Scholar 

  • Hanisak, M. D., 1983. The nitrogen relationships of marine macroalgae. In E. J. Carpenter & D. G. Capone (eds) Nitrogen in the Marine Environment. Academic Press, New York: 699–730.

    Google Scholar 

  • Hanisak, M. D. & M. M. Harlin, 1978. Uptake of inorganic nitrogen by Codium fragile subsp. tomentosoides (Chlorophyta). J. Phycol. 14: 450–454.

    Google Scholar 

  • Harrison, P. J., 1988. Determining phosphate uptake rates of phytoplankton. In C. S. Lobban, D. J. Chapman & B. P. Kremer (eds) Experimental Phycology — A Laboratory Manual. Cambridge University Press, Cambridge: 186–195.

    Google Scholar 

  • Harrison, P. J. & L. D. Druehl, 1982. Nutrient uptake and growth in the Laminariales and other macrophytes: a consideration of methods. In L. M. Srivastava (ed.) Synthetic and Degradative Processes in Marine Macrophytes. Walter de Gruyter & Co., Berlin: 99–120.

    Google Scholar 

  • Harrison, P. J., L. D. Druehl, K. E. Lloyd & P. A. Thompson, 1986. Nitrogen uptake kinetics in three year-classes of Laminaria groenlandica (Laminariales: Phaeophyta). Mar. Biol. 93: 29–35.

    Google Scholar 

  • Healey, E. P., 1980. Slope of the Monod equation as an indicator of advantage in nutrient competition. Microb. Ecol. 5: 281–286.

    Google Scholar 

  • Hurd, C. L. & M. J. Dring, 1990. Phosphate uptake by intertidal algae in relation to zonation and season. Mar. Biol. 107: 281–289.

    Google Scholar 

  • Hurd, C. L., R. S. Galvin, T. A. Norton & M. J. Dring, 1993. Production of hyaline hairs by intertidal species of Fucus (Fucales) and their role in phosphate uptake. J. Phycol. 29: 160–165.

    Google Scholar 

  • Jackson, G. A., 1977. Nutrients and production of giant kelp, Macrocystis pyrifera, off southern California. Limnol. Oceanogr. 22: 979–995.

    Google Scholar 

  • Joly, A. B. & E. C. de Oliveira Filho, 1967. Two Brazilian Laminaria. Publicações do Instituto de Pesquisas da Marinha, RJ 4: 1–13.

  • Kautsky, L., 1982. Primary production and uptake kinetics of ammonium and phosphate by Enteromorpha compressa in an ammonium sulphate industry outlet area. Aquat. Bot. 12: 23–40.

    Google Scholar 

  • Kopczak, C. D., 1994. Variability of nitrate uptake capacity in Macrocystis pyrifera (Laminariales, Phaeophyta) with nitrate and light availability. J. Phycol. 30: 573–580.

    Google Scholar 

  • Lapointe, B. E., 1987. Phosphorus- and nitrogen-limited photosynthesis and growth of Gracilaria tikvahiae (Rhodophyta) in the Florida Keys: an experimental field study. Mar. Biol. 93: 561–568.

    Google Scholar 

  • Lindner, E., C. A. Dooley & R. H. Wade, 1977. Chemical Variation of Chemical Constituents in Macrocystis pyrifera. Ocean Food Energy Farm Project Final Report. Naval Undersea Center, San Diego, California, 25 pp.

    Google Scholar 

  • Lobban, C. S., P. J. Harrison & M. J. Duncan, 1985. The Physiological Ecology of Seaweds. Cambridge University Press, Cambridge, 242 pp.

    Google Scholar 

  • Manley, S. L., 1985. Phosphate uptake by blades of Macrocystis pyrifera (Phaeophyta). Bot. mar. 28: 237–244.

    Google Scholar 

  • Manley, S. L. & W. J. North, 1984. Phosphorus and the growth of juvenile Macrocystis pyrifera (Phaeophyta) sporophytes. J. Phycol. 20: 389–393.

    Google Scholar 

  • Nissen, P., 1974. Uptake mechanisms: Inorganic and Organic. Annu. Rev. Plant Physiol. 25: 53–79.

    Google Scholar 

  • North, W. J., V. A. Gerard & J. S. Kuwabara, 1982. Farming Macrocystis at coastal and oceanic sites. In L. M. Srivastava (ed.) Synthetic and Degradative Processes in Marine Macrophytes. Walter de Gruyter & Co., Berlin: 247–262.

    Google Scholar 

  • Pedersen, M. F., 1994. Transient ammonium uptake in the macroalga Ulva lactuca (Chlorophyta): nature, regulation, and the consequences for choice of measuring technique. J. Phycol. 30: 980–986.

    Google Scholar 

  • Probyn, T. A. & A. R. O. Chapman, 1982. Nitrogen uptake characteristics of Chordaria flagelliformis (Phaeophyta) in batch mode and continuous mode experiments. Mar. Biol. 71: 129–133.

    Google Scholar 

  • Provasoli, L., 1968. Media and prospects for cultivation of marine algae. In A. Watanabe & A. Hattori (eds) Cultures and Collections of Algae. Japanese Society of Plant Physiology, Tokyo: 47–74.

  • Quège, N., 1988. Laminaria (Phaeophyta) no Brasil, uma perspectiva econômica. M. Sc. Dissertation. Universidade de São Paulo, 230 pp.

  • Smith, S. V., 1984. Phosphorus versus nitrogen limitation in the marine environment. Limnol. Oceanogr. 29: 1149–1160.

    Google Scholar 

  • Solórzano, L., 1969. Determination of ammonia in natural waters by the phenolhypochlorite methods. Limnol. Oceanogr. 14: 799–801.

    Google Scholar 

  • Steffensen, D. A., 1976. The effect of nutrient enrichment and temperature on the growth in culture of Ulva lactuca L. Aquat. Bot. 2: 337–351.

    Google Scholar 

  • Strickland, J. D. H. & T. R. Parsons, 1972. A practical handbook of seawater analysis. Bull. Fish. Res. Bd Can. 167: 71–89.

    Google Scholar 

  • Topinka, J. A., 1978. Nitrogen uptake by Fucus spiralis. J. Phycol. 14: 241–247.

    Google Scholar 

  • Wallentinus, I., 1981. Chemical constituents of some baltic macroalgae in relation to environmental conditions. Proc. Int. Seaweed Symp. 10: 363–370.

    Google Scholar 

  • Wallentinus, I., 1984. Comparisons of nutrient uptake rates for Baltic macroalgae with different thallus morphologies. Mar. Biol. 80: 215–225.

    Google Scholar 

  • Wheeler, W. N., 1978. Ecophysiological studies in the giant kelp Macrocystis. Ph. D. thesis, Univ. Calif. Santa Barbara Ca, 179 pp.

    Google Scholar 

  • Wheeler, W. N., 1982. Nitrogen nutrition of Macrocystis. In L. M. Srivastava (ed.) Synthetic and Degradative Processes in Marine Macrophytes. Walter de Gruyter & Co., Berlin: 121–137.

    Google Scholar 

  • Wheeler, P. A. & W. J. North, 1980. Effect of nitrogen supply on nitrogen content and growth rate of juvenile Macrocystis pyrifera (Phaeophyta) sporophytes. J. Phycol. 16: 577–582.

    Google Scholar 

  • Yoneshigue-Valentin, Y, 1990. The life cycle of Laminaria abyssalis (Laminariales, Phaeophyta) in culture. Hydrobiologia 204/205: 461–466.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Universidade Federal do Rio de Janeiro, Cidade Universitaria, Ilha do Fundão, Instituto de Biofisica Carlos Chagas Filho, 21944-590, Rio de Janeiro, Brazil

    Adriana dā Costa Braga

  2. Departamento de Botânica, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Cidade Universitária, Ilha do Fundão, 21944-590, Rio de Janeiro, Brazil

    Yocie Yoneshigue-Valentine

  3. Departamento de Pesca e Aquicultura, Universidade do Rio de Janeiro (UNI-RIO), rua Frei Caneca, 94/5°, Centro, 22211-030, Rio de Janeiro, Brazil

    Adriana dā Costa Braga

Authors
  1. Adriana dā Costa Braga
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yocie Yoneshigue-Valentine
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Braga, A.d.C., Yoneshigue-Valentine, Y. Nitrogen and phosphorus uptake by the Brazilian kelp Laminaria abyssalis (Phaeophyta) in culture. Hydrobiologia 326, 445–450 (1996). https://doi.org/10.1007/BF00047844

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00047844

Key words

Search

Navigation