Abstract
Four winter wheat (Triticum aestivum L.) and two spring wheat cultivars were evaluated in anther culture on three to four different media for their ability to initiate callus and green plants. Five media were used in the experiment: stored-potato medium with Ficoll 400, fresh-potato medium with Ficoll 400, fresh-potato medium with agar, fresh-potato liquid medium without agar or Ficoll 400, and a one tep 85D12-3 medium. Greatly different frequencies of calli and/or green plants were obtained from different cultivars and media. The callus initiation frequency varied from 2.7% for Arapahoe to 52% for Pavon, both on the stored potato medium with Ficoll 400. The frequency of green plant regeneration ranged from 0% for Arapahoe and Siouxland on the stored-potato medium with Ficoll 400 and 0% for Redland and Arapahoe in the fresh-potato medium with Ficoll 400 to 12% for Chris in the 85D12-3 medium (one-step procedure). Chris and Centurk 78, previously reported as having high levels of response, had significantly higher (P < 0.05) frequencies of green plant regeneration on the 851312-3 medium than the other cultivars. An unexpected observation is that ‘wet’ MSC− medium enhanced callus regeneration more than a drier MSC− medium.
Similar content being viewed by others
References
Armstrong TA, Metz SG, Mascia PN (1987) Two regeneration systems for the production of haploid plants from wheat anther culture. Plant Sci 51: 231–237
Baenziger PS, Kudirka DT, Schaeffer GW, Lazar MD (1984) The significance of doubled haploid variation In: Gustafson et al JP Gene manipulation in plant improvement (pp 385–414) Plenum Publ Corp
Deaton WR, Metz SG, Armstrong TA, Mascia PN (1987) Genetic analysis of the anther culture-response of three spring wheat crosses. Theor Appl Genet 74: 334–338
Huang B (1987) Effects of incubation temperature on microspore callus production and plant regeneration in wheat anther cultures. Plant Cell Tissue Organ Culture 9: 45–48
Jones AM, Petolino JF (1987) Effects of donor plant genotype and growth environment on anther culture of soft-red winter wheat (Triticum aestivum L.) Plant Cell Tissue Organ Culture 8: 215–223
Lazar MN, Baenziger PS, Schaeffer GW (1984) Combining abilities and heritability of callus formation and plantlet regeneration in wheat (Triticum aestivum L.) anther cultures. Theor Appl Genet 68: 131–134
Lazar MD, Schaeffer GW, Baenziger PS (1985) The physical environment in relation to high-frequency callus and plantlet development in anther cultures of wheat (Triticum aestivum L.) cv. Chris. J Plant Physiol 121: 103–109
Li H, Qureshi JA, Kartha KK (1988) The influence of different temperature treatments on anther culture response of spring wheat (Triticum aestivum L.) Plant Sci 57: 55–61
Liang GH, Xu A, Tang-Huang (1987) Direct regeneration of wheat haploids via anther culture. Crop Sci 27: 336–339
Marsolais AA, Swartz-Seguin G, Kasha KJ (1984) The influence of anther cold pretreatments and donor plant genotypes on in vitro androgenesis in wheat (Triticum aestivum L.). Plant Cell Tissue Organ Culture 3: 69–79
Mathias RJ, Simpson ES (1986) The interaction of genotype and culture medium on the tissue culture responses of wheat (Triticum aestivum L em Thell) callus. Plant Cell Tissue Organ Culture 7: 31–37
Ouyang JW, He DD, Feng GH, Jia SE (1987) The response of anther culture to culture temperature varies with growth conditions of anther-donor plants. Plant Sci 49: 145–149
Steel RGD, Torrie JH (1980) Principles and procedures of statistics. McGraw-Hill Book Comp, New York
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Han-min, Y., Keppenne, V.D., Baenziger, P.S. et al. Effect of genotype and medium on wheat (Triticum aestivum L.) anther culture. Plant Cell Tiss Organ Cult 21, 253–258 (1990). https://doi.org/10.1007/BF00047618
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00047618