Skip to main content
Log in

A microcomputer program and fast analog to digital converter card for the analysis of fluorescence induction transients

  • Technical Communication
  • Published:
Photosynthesis Research Aims and scope Submit manuscript

Abstract

A microcomputer program and an analog to digital conversion card were developed for the analysis of fluorescence induction curves. The program and the analog to digital conversion card are compatible with all commercially available fluorometers. Most of the current analysis methods for fluorescence induction curves are implemented in the program, including analysis of OIDPSMT-kinetics, dissection of fluorescence quenching into two components, measurement of the slope of the fluorescence curve, complementary area analysis and analysis of energy ‘spillover’ from PS II to PS I. The program can also do basic statistical calculations from the measured parameters. The architecture of the program is open, allowing the user to add new methods to the main body of the program. Split time-scale is used in data capture and analysis. A new procedure facilitates accurate determination of F0.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Barber J (1982a) The control of membrane organization by electrostatic forces. Biosci Rep 2: 1–13

    Google Scholar 

  • Barber J (1982b) Influence of surface charges on thylakoid stucture and function. Ann Rev Plant Physiol 33: 261–295

    Google Scholar 

  • Bell DH and Hipkins MF (1985) Analsis of fluorecence induction curves from pea chloroplasts. Photosystem II reaction centre heterogeneity. Biochim Biophys Acta 807: 255–262

    Google Scholar 

  • Briantais JM, Vernotte C, Krause GH and Weis E (1986) Chlorophyll a fluorescence of higher plants: chloroplasts and leaves. In: Govindjee, Amesz J and Fork DC (eds) Light Emission by Plants and Bacteria, pp. 539–583. Orlando: Academic Press

    Google Scholar 

  • Butler WL (1978) Energy distribution in the photochemical apparatus of photosynthesis. Ann Rev Plant Physiol 29: 345–378

    Google Scholar 

  • Krause GH and Weis E (1984) Chlorophyll fluorescence as a tool in plant physiology. II. Interpretation of fluorescence signals. Photosynth Res 5: 139–157

    Google Scholar 

  • Lichtenthaler HK (ed) (1988) Applications of Chlorophyll Fluorescence in Photosynthesis Research, Stress Physiology, Hydrobiology and Remote Sensing. 366 s, Kluwer Academic Publishers: Dordrecht

    Google Scholar 

  • Melis A, Guenther GE, Morrissey PJ and Ghirardi ML (1988) Photosystem II heterogeneity in chloroplasts. In: Lichtenthaler HK (ed) Applications of Chlorophyll Fluorescence in Photosynthesis Research, Stress Physiology, Hydrobiology and Remote Sensing, pp 33–43. Kluwer Academic Publishers: Dordrecht

    Google Scholar 

  • Melis A and Homann PH (1975) Kinetic analysis of the fluorescence induction in 3-(3,4-dichlorophenyl)-1,1-dimethylurea poisoned chloroplasts. Photochem Photobiol 21: 431–437

    Google Scholar 

  • Melis A and Homann PH (1978) A selective effect of Mg2+ on the photochemistry at one type of reaction center in Photosystem II of chloroplasts. Arch Biochem Biophys 190: 523–530

    Google Scholar 

  • Morissette JC and Popovic R (1987) A new method for the separation of the constant and the variable fluorescence of chlorophyll a in in vivo. Biochim Biophys Res Commun 149: 385–390

    Google Scholar 

  • Norrish R, Kriedemann PE and Wiskich JT (1983) Chlorophyll a fluorescence transients: a fast data acquisition system to facilitate in vivo measurements. Photosynth Res 4: 213–227

    Google Scholar 

  • Renger G and Schreiber U (1986) Practical applications of fluorometric methods to algae and higher plant research. In: Govindjee, Amesz J and Fork DC (eds) Light Emission by Plants and Bacteria, pp 587–619. Orlando: Academic Press

    Google Scholar 

  • Schreiber U (1986) Detection of rapid induction kinetics with a new type of high-frequency modulated chlorophyll fluorometer. Photosynth Res 9: 261–272

    Google Scholar 

  • Schreiber U, Groberman L and Vidaver W (1975) Portable, solid-state fluorometer for the measurement of chlorophyll fluorescence induction in plants. Rev Sci Instrum 46: 538–542

    Google Scholar 

  • Schreiber U, Schliwa U and Bilger W (1986) Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynth Res 10: 51–62

    Google Scholar 

  • Sinclair I and Spence SM (1988) The analysis of fluorescence induction transients from dichlorophenyldimethylurea-poisoned chloroplasts. Biochim Biophys Acta 935: 184–194

    Google Scholar 

  • Smillie RM and Hetherington SE (1983) Stress tolerance and stress-induced injury in crop plants measured by chlorophyll fluorescence in vivo. Plant Physiol 72: 1043–1050

    Google Scholar 

  • Tyystjärvi E, Ovaska J, Karunen P and Aro E-M (1989) The nature of light-induced inhibition of Photosystem II in pumpkin (Cucurbita pepo L.) leaves depends on temperature. Plant Physiol 91: 1069–1074

    Google Scholar 

  • Walker DA (1988) The Use of The Oxygen Electrode and Fluorescence Probes in Simple Measurements of Photosynthesis. Sheffield: Oxygraphics Ltd.

    Google Scholar 

  • Ögren E and Baker NR (1985) Evaluation of a technique for the measurement of chlorophyll fluorescence from leaves exposed to continuous white light. Plant Cell Environ 8: 539–547

    Google Scholar 

  • Öquist G and Wass R (1988) A portable, microprocessor operated instrument for measuring chlorophyll fluorescence kinetics in stress physiology. Physiol Plant 73: 211–217

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tyystjärvi, E., Karunen, J. A microcomputer program and fast analog to digital converter card for the analysis of fluorescence induction transients. Photosynth Res 26, 127–132 (1990). https://doi.org/10.1007/BF00047084

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00047084

Key words

Navigation