Abstract
Three unselected cell lines of C. roseus maintained on a growth-associated alkaloid production medium were studied over a period of 2 to 5.5 years for the stability of alkaloid production (serpentine and ajmalicine). Large fluctuations in the total alkaloid content of 20-day-old cells were found for all three cell lines at each subculture over a two-year period. Growth rates increased during prolonged subculture and one cell line became unproductive after five years culture. By selection of small autofluorescent aggregates, high alkaloid production was restored in this cell line, while the parent line was found to be unresponsive to alkaloid induction treatments. The instability in both alkaloid production and spectrum and the loss of alkaloid productivity are discussed in relation to the selection pressures present during long-term maintenance of cell suspension cultures.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- nHS:
-
n-heptane sulphonate
References
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Morris, P., Rudge, K., Cresswell, R. et al. Regulation of product synthesis in cell cultures of Catharanthus roseus. V. Long-term maintenance of cells on a production medium. Plant Cell Tiss Organ Cult 17, 79–90 (1989). https://doi.org/10.1007/BF00046853
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DOI: https://doi.org/10.1007/BF00046853