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Structure, function and biological properties of integrin αvβ3on human melanoma cells

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Summary

Human melanoma represents one of the most metastatic cancers in man. The capacity of melanoma cells to invade a variety of tissues and extracellular matrices is, in part, due to their repertoire of adhesion receptors. To this end, human melanoma cells express multiple integrin cell adhesion receptors among these is the vitronectin receptor, αvβ3. This adhesion receptor enables melanoma cells to attach to a wide variety of extracellular matrix components containing the sequence Arg-Gly-Asp. This review will focus on the biosynthetic, biochemical and biological properties of this receptor expressed on the surface of human melanoma cells.

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References

  1. Hynes, RO: Integrins: a family of cell surface receptors. Cell 48: 549–554, 1987

    Google Scholar 

  2. Chen, WT, Hasegawa, T, Hasegawa, C, Weinstock, C, Yamada, KM: Development of cell surface lineage complexes in cultivated fibroblasts. J Cell Biol 100: 1103–1114, 1985

    Google Scholar 

  3. Damsky, CM, Knudsen, KA, Bradley, D, Buck, CA, Horwitz, A: Distribution of the CSAT cell-matrix antigen on myogenic and fibroblastic cells in culture. J Cell Biol 100: 1528–1539, 1985

    Google Scholar 

  4. Burridge, K, Fath, K, Kelly, T, Nuckolls, G, Turner, C: Focal adhesions: transmembrane junctions between the extracellular matrix and the cytoskeleton. Annu Rev Cell Biol 4: 487–525, 1988

    Google Scholar 

  5. Dejanna, E, Colella, S, Conforti, G, Abbadini, M, Gaboli, M, Marchisio, PC: Fibronectin and vitronectin regulate the organization of their respective Arg-Gly-Asp adhesion receptors in cultured human endothelial cells. J Cell Biol 107: 1215–1223, 1988

    Google Scholar 

  6. Singer, II, Scott, S, Kawka, DW, Kazazis, DM, Gailit, J, Rouslahti, E: Cell surface distribution of fibronectin and vitronectin receptors depends on substrate composition and extracellular matrix accumulation. J Cell Biol 106: 2171–2182, 1988

    Google Scholar 

  7. Carter, WG, Wayner, EA, Bouchard, TS, Kaur, P: The role of integrins α3β1 and α3β1 in cell-cell and cell-substrate adhesion of human epidermal cells. J Cell Biol 110: 1387–1404, 1990

    Google Scholar 

  8. Geiger, B: A 130-K protein from chicken gizzard: Its localization at the termini of microfilament bundles in cultured chicken cells. Cell 18: 193–205, 1979

    Google Scholar 

  9. Horwitz, A, Duggan, K, Buck, CA, Beckerle, MC, Burridge, K: Interaction of plasma membrane fibronectin receptor with talin—a transmembrane linkage. Nature (Lond) 320: 531–533, 1986

    Google Scholar 

  10. Tapley, P, Horwitz, AF, Buck, CA, Burridge, K, Duggan, K, Hirst, R, Rohrschneider, L: Analysis of the avian fibronectin receptor (integrin) as direct substrate for pp60 v-src. Oncogene 4: 325–333, 1989

    Google Scholar 

  11. Nuckolls, GH, Turner, CE, Burridge, K: Functional studies of the domains of talin. J Cell Biol 110: 1635–1644, 1990

    Google Scholar 

  12. Otey, CA, Pavalko, FM, Burridge, K: An interaction between α-actinin and the β1 integrin subunit in vitro. J Cell Biol 111: 721–729, 1990

    Google Scholar 

  13. Cheresh, DA, Spiro, RC: Biosynthetic and functional properties of an Arg-Gly-Asp-directed receptor involved in human melanoma cell attachment to vitronectin, fibrinogen and von Willebrand factor. J Biol Chem 262: 17703–17711, 1987

    Google Scholar 

  14. Cheresh, DA: Human endothelial cells synthesize and express an Arg-Gly-Asp-directed receptor involved in attachment to fibrinogen and von Willebrand factor. Proc Natl Acad Sci USA 84: 6471–6475, 1987

    Google Scholar 

  15. Lawler, J, Weinstein, R, Hynes, RO: Cell attachment to thrombospondin: the role of RGD and integrin receptors. J Cell Biol 107: 2351–2361, 1988

    Google Scholar 

  16. Reinholt, FP, Hultenby, K, Oldberg, A, Heinegard, D: Osteopontin—a possible anchor of osteoclasts to bone. Proc Natl Acad Sci USA 87: 4473–4475, 1990

    Google Scholar 

  17. Charo, IF, Nannizzi, L, Smith, JW, Cheresh, DA: The vitronectin receptor α5β1 binds fibronectin and acts in concert with αvβ3 in promoting cellular attachment and spreading on fibronectin. J Cell Biol 111: 2795–2800, 1991

    Google Scholar 

  18. Kramer, RH, Cheng, Y-F, Clyman, R: Human microvascular endothelial cells use β1 and β3 integrin receptor complexes to attach to laminin. J Cell Biol 111: 1223–1243, 1990

    Google Scholar 

  19. Cheresh, DA, Berliner, SA, Vicente, C, Ruggeri, ZM: Recognition of distinct adhesive sites on fibrinogen by related integrins on platelets and endothelial cells. Cell 58: 945–953, 1989

    Google Scholar 

  20. Smith, JW, Ruggeri, ZM, Kunicki, TJ, Cheresh, DA: Interaction of Integrins αvβ3 and glycoprotein IIb–IIIa with fibrinogen. Differential peptide recognition accounts for distinct binding sites. J Biol Chem 265: 12267–12271, 1990

    Google Scholar 

  21. Albeda SM, Mette SA, Elder DE, Stewart R, Damjanovich L, Herlyn M, Buck CA: Integrin distribution in malignant melanoma: Association of the β3 subunit with tumor progression. Cancer Res 1990

  22. Bodary, SC, McClean, JW: The integrin β1 subunit associates with the vitronectin receptor 10–4 subunit to form a novel vitronectin receptor in a human embryonic kidney cell line. J Biol Chem 265: 5938–5941, 1990

    Google Scholar 

  23. Vogel, BE, Tarone, G, Giancotti, FG, Gailit, J, Ruoslahti, E: A novel fibronectin receptor with an unexpected subunit composition αvβ1. J Biol Chem 265: 5934–5937, 1990

    Google Scholar 

  24. Cheresh, DA, Smith, JW, Cooper, HM, Quaranta, V: A novel vitronectin receptor integrin αvβx is responsible for distinct adhesive properties of carcinoma cells. Cell 57: 59–69, 1989

    Google Scholar 

  25. Smith, JW, Vestal, DJ, Irwin, SV, Burke, T, Cheresh, DA: Purification and functional characterization of integrin αvβ5: An adhesion receptor for vitronectin. J Biol Chem 256: 11008–11013, 1990

    Google Scholar 

  26. Ramaswamy, Hemler, ME: Cloning, primary structure and properties of a novel human integrin β subunit. EMBO J 9: 1561–1568, 1990

    Google Scholar 

  27. McClean, JW, Vestal, DJ, Cheresh, DA, Bodary, SC: cDNA sequence of the human integrin β5 subunit. J Biol Chem 265: 17126–17131, 1990

    Google Scholar 

  28. Boukerche, H, Berthier-Vergnes, O, Bailly, M, Dore, JF, Leung, LK, McGregor, JL: A monoclonal antibody (LYP18) directed against the blood platelet glycoprotein IIb/IIIa complex inhibits human melanoma growth in vivo. Blood 74: 909–912, 1989

    Google Scholar 

  29. Gehlson, KR, Argraves, WS, Pierschbacher, MD, Ruoslahti, E: Inhibition of in vitro tumor invasion by Arg-Gly-Asp-containing peptides. J Cell Biol 106: 925–930, 1988

    Google Scholar 

  30. Humphries, MJ, Olden, K, Yamada, KM: A synthetic peptide from fibronectin inhibits experimental metastasis of murine melanoma cells. Science 233: 467–470, 1986

    Google Scholar 

  31. Saiki, I, Murata, J, Lida, J, Nishi, N, Sugimura, K, Azuma, I: The inhibition of murine lung metastasis by synthetic polypeptides [poly (RGD) and poly (YIGSR)] with a core sequence of cell adhesion molecules. Br J Cancer 59: 194–197, 1988

    Google Scholar 

  32. Humphries, MJ, Yamada, KM, Olden, K: Investigation of the biological effects of the anti-cell adhesion synthetic peptides that inhibit experimental metastasis of B16-F10 murine melanoma cells. J Clin Invest 81: 782–790, 1988

    Google Scholar 

  33. Cheresh, DA, Harper, JA: Arg-Gly-Asp recognition by a cell adhesion receptor requires its 130 kDa α-subunit. J Biol Chem 262: 1434–1437, 1987

    Google Scholar 

  34. Smith, JW, Cheresh, DA: The RGD binding domain of the vitronectin receptor: Photoaffinity crosslinking implicates residues 61–203 of the β subunit. J Biol Chem 263: 18726–18731, 1988

    Google Scholar 

  35. Smith, JW, Cheresh, DA: Integrin αvβ3-ligand interaction: Identification of a divalent cation-dependent, heterodimeric RGD binding site on the vitronectin receptor. J Biol Chem 256: 2168–2172, 1990

    Google Scholar 

  36. D'Souza, SE, Ginsberg, MH, Lam, S, Plow, E: Chemical crosslinking of arginyl-glycyl-aspartic acid peptides on adhesion receptors on platelets. Science 242: 91–93, 1988

    Google Scholar 

  37. Cheresh, DA, Pierschbacher, MD, Herzig, MA, Mujoo, K: Disialogangliosides GD2 and GD3 are involved in the attachment of human melanoma and neuroblastoma cells to extracellular matrix proteins. J Cell Biol 102: 688–696, 1986

    Google Scholar 

  38. Cheresh, DA, Klier, FG: Disialoganglioside GD2 distributes preferentially into substrate-associated microprocesses on human melanoma cells during their attachment to fibronectin. J Cell Biol 102: 1887–1897, 1986

    Google Scholar 

  39. Cheresh, DA, Pytela, R, Klier, FG, Pierschbacher, MD, Ruoslahti, E, Reisfeld, RA: An Arg-Gly-Asp-directed receptor on the surface of human melanoma cells exists in a functional complex with the disialoganglioside GD2. J Cell Biol 105: 1163–1173, 1987

    Google Scholar 

  40. Stallcup, WB: Involvement of gangliosides and glycoprotein fibronectin receptors in cellular adhesion to fibronectin. Exp Cell Res 177: 90–102, 1988

    Google Scholar 

  41. Conforti, G, Zanetti, A, Pasquali-Ronchetti, I, Quaglino, D, Neyroz, P, Dejanna, E: Modulation of vitronectin receptor binding by membrane lipid composition. J Biol Chem 265: 4011–4019, 1990

    Google Scholar 

  42. Brown E, Hooper L, Ho T, Gresham H: Integrin-associated protein: A 50-kD plasma membrane antigen physically and functionally associated with integrins. J Cell Biol 1990, in press

  43. Wayner EA, Orlando RA, Cheresh DA: Integrins αvβ3 and αvβ5 contribute to cell attachment to vitronectin but differentially distribute on the cell surface. J Cell Biol 1991, in press

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  1. Dept. of Immunology, Research Institute of Scripps Clinic, 10666 N. Torrey Pines Rd., 92037, LaJolla, CA, USA

    David A. Cheresh

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Cheresh, D.A. Structure, function and biological properties of integrin αvβ3on human melanoma cells. Cancer Metast Rev 10, 3–10 (1991). https://doi.org/10.1007/BF00046839

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