Abstract
Sour cherry cv. Šumadinka (Prunus cerasus L.) is the leading Yugoslav cultivar for production orchards. A method of micropropagation has been developed for the purpose of growing ‘Šumadinka’ on its own roots and for rapid multiplication.
Aseptic cultures were initiated from shoot explants 1–2 mm long on Murashige & Skoog medium with (in mgl-1) 6-benzylaminopurine (BAP): 1, indole-3-yl butyric acid (IBA): 1 and gibberellic acid (GA3): 0–1.
The best medium for proliferation was MS with (in mgl-1): BAP 0.5, IBA 0.1, GA3 0.1, but media with (in mgl-1): BAP 0.5, NAA 0.1, GA3 0.1 and BAP 1, NAA 0.1 and GA3 0.1 were also shown to be good. A higher degree of proliferation obtained with some media did not necessarily result in a better quality of plantlets produced.
For rooting the best combination of culture medium was achieved with pretreatment 10 days in MS 1/2 with 1 mgl-1 IBA, followed by transfer to a hormone-free medium after 5–10 days, resulting in 88% success.
The rooted plants were planted in containers and acclimatized under mist, with over 90% of plants surviving transplantation.
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Cerović, R., Ružić, D. Micropropagation of sour cherry (Prunus cerasus L.) cv. Šumadinka. Plant Cell Tiss Organ Cult 9, 151–157 (1987). https://doi.org/10.1007/BF00044251
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DOI: https://doi.org/10.1007/BF00044251