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Isolation of a poplar and anArabidopsis thaliana dihydrodipicolinate synthase cDNA clone

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Abstract

A poplar DHDPS cDNA clone has been isolated by functional rescue of thedapA-deficient AT997 mutant ofEscherichia coli. By sequence comparison between the poplar and maize DHDPS cDNAs, two oligonucleotides were designed to perform polymerase chain reaction (PCR) onArabidopsis thaliana genomic DNA. The PCR fragment was subsequently used to isolate anArabidopsis DHDPS genomic and cDNA clone.

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References

  1. Negrutiu J, Cattoir-Reynearts A, Verbruggen I, Jacobs M: Lysine overproducer mutants with an altered dihydrodipicolinate synthase from protoplast culture ofNicotiana sylvestris. Theor Applied Genet 68: 11–20 (1984).

    Google Scholar 

  2. Vernaillen S, Van Ghelue M, Verbruggen I, Jacobs M: Characterization of mutants inArabidopsis thaliana resistant to analogues and inhibitory concentrations of amino acids derived from aspartate. Arabidopsis Inf Serv 22: 13–20 (1985).

    Google Scholar 

  3. Frankard V, Ghislain M, Jacobs M: Two feedback-insensitive enzymes of the aspartate pathway inNicotiana sylvestris. Plant Physiol 99: 1285–1293 (1992).

    Google Scholar 

  4. Kumpaisal R, Hashimoto T, Yamada Y: Purification and characterization of dihydrodipicolinate synthase from wheat suspension cultures. Plant Physiol 85: 145–151 (1987).

    Google Scholar 

  5. Frisch D, Gengenbach B, Tommey A, Sellner J, Somers D, Myers D: Isolation and characterization of dihydrodipicolinate synthese from maize. Plant Physiol 96: 444–452 (1991).

    Google Scholar 

  6. Ghislain M, Frankard V, Jacobs M: Dihydrodipicolinate synthase ofNicotiana sylvestris, a chloroplast-localized enzyme of the lysine pathway. Planta 180: 480–486 (1990).

    Google Scholar 

  7. Laber B, Gomis-Rüth F, Romao M, Huber R:Escherichia coli dihydrodipicolinate synthase: identification of the active site and crystallization. Biochem J 288: 691–695 (1992).

    Google Scholar 

  8. Perl A, Shaul O, Galili G: Regulation of lysine synthesis in transgenic potato plants expressing a bacterial dihydrodipicolinate synthase in their chloroplasts. Plant Mol Biol 19: 815–823 (1992).

    Google Scholar 

  9. Shaul O, Galili G: Increased lysine synthesis in tobacco plants that express high levels of bacterial dihydrodipicolinate synthase in their chloroplasts. Plant J: 203–209 (1992).

  10. Taneko T, Hashimoto T, Kumpaisal R, Yamada Y: Molecular cloning of wheat dihydrodipicolinate synthase. J Biol Chem 265: 17451–17455 (1990).

    Google Scholar 

  11. Frish D, Tommey A, Gengenbach B, Somers D: Direct genetic selection of a maize cDNA for dihydrodipicolinate synthase in anEscerichia coli dapA auxotroph. Mol Gen Gent 228: 287–293 (1991).

    Google Scholar 

  12. Richaud F, Richaud C, Ratet P, Patte JC: Chromosomal location and nucleotide sequence of theEscherichia coli dapA gene. J Bact 166: 297–300 (1986).

    Google Scholar 

  13. Bonnassie S, Oreglia J, Sicard A: Nucleotide sequence of thedapA gene fromCorynebacterium glutamicum. Nucl Acids Res 18: 6421 (1990).

    Google Scholar 

  14. Nai-Young Chen, Shu-Qin Jiang, Klein D, Paulus H: Organization and nucleotide sequence of theBacillus subtilis diaminopimelate operon, a cluster of genes encoding the first three enzymes of diaminopimelate synthesis and dipicolinate synthase. J Biol Chem 268: 9448–9465 (1993).

    Google Scholar 

  15. Dumas B, Van Doorsselaere J, Gielen J, Legrand M, Fritig B, Van Montagu M, Inzé D: Nucleotide sequence of a complementary DNA encodingO-methyltransferase from poplar. Plant Physiol 98: 796–797 (1992).

    Google Scholar 

  16. Feinberg A, Vogelstein B: A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13 (1983).

    Google Scholar 

  17. Lütcke H, Chow K, Mickel F, Moss K, Kern H, Schele G: Selection of AUG initiation codons differs in plants and animals. EMBO J 6: 43–48 (1987).

    Google Scholar 

  18. Gavel Y, von Heije G: A conserved cleavage-site motif in chloroplast transit peptides. FEBS Lett 261: 455–458 (1990).

    Google Scholar 

  19. von Heijne G, Steppuhn J, Herrmann R: Domain structure of mitochondrial and chloroplast targeting peptides. Eur J Biochem 180: 535–545 (1989).

    Google Scholar 

  20. Shedlarsky J, Gilvarg C: The pyruvate-aspartic semialdehyde condensing enzyme ofEscherichia coli. J Biol Chem 245: 1362–1373 (1970).

    Google Scholar 

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Authors and Affiliations

  1. Laboratorium voor Plantengenetica, Vrije Universiteit Brussel, Paardenstraat 65, B-1640, St.-Genesius Rode, Belgium

    Marc Vauterin & Michel Jacobs

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  1. Marc Vauterin
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  2. Michel Jacobs
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Vauterin, M., Jacobs, M. Isolation of a poplar and anArabidopsis thaliana dihydrodipicolinate synthase cDNA clone. Plant Mol Biol 25, 545–550 (1994). https://doi.org/10.1007/BF00043882

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  • DOI: https://doi.org/10.1007/BF00043882

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