Abstract
The rice glutelin Gt3 promoter was fused to a β-glucuronidase (GUS) reporter gene and its expression evaluated in transgenic tobacco plants. Histochemical analysis revealed that the expression of the introduced Gt3 promoter/GUS (β-glucuronidase) chimeric gene was confined to endosperm tissue of developing seeds. 5′-promoter deletion analysis revealed that two domains of the Gt3 promoter, -346 to -263 bp (domain I) and -945 to -726 bp (domain II) from the transcriptional start site, were essential for optimum expression of the GUS reporter gene. Removal of 5′ sequences upstream of -726 resulted in a reduction in overall promoter activity and a shift in temporal expression from a maximum of 16–20 days after flowering to 24 days. Removal of DNA sequences from the 5′ end to -346 yielded a promoter fragment that was still able to confer endosperm-specific expression, although a further deletion to -263 abolished promoter activity. These data suggest that at least twocis-regulatory elements are required for endosperm specificity and temporal regulation of glutelin Gt3 gene expression.
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Zhao, Y., Leisy, D.J. & Okita, T.W. Tissue-specific expression and temporal regulation of the rice glutelin Gt3 gene are conferred by at least two spatially separatedcis-regulatory elements. Plant Mol Biol 25, 429–436 (1994). https://doi.org/10.1007/BF00043871
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DOI: https://doi.org/10.1007/BF00043871