Abstract
Twelve cDNAs corresponding to mRNAs inducible by ethylene were isolated by differential screening of a cDNA library from ethylene-treated Citrus sinensis fruits. Northern analysis of RNA extracted from flavedo of ethylene-treated fruits and from fruits at different maturation stages showed that some of the mRNAs corresponding to these cDNAs were regulated both by ethylene treatment and during fruit maturation. The effect of exogenous ethylene on leaves and of endogenous ethylene on flowers showed that gene induction was not restricted to the flavedo tissue. The possible role of ethylene during maturation of the non-climacteric Citrus fruit is discussed.
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References
Abeles FB: Ethylene in Plant Biology. Academic Press, New York (1973).
Alonso JM, García-Martínez JL, Chamarro J: Two dimensional gel electrophoresis pattern of total, in vivo labeled and in vitro translated polypeptides from organe flavedo during maturation and following ethylene treatment. Physiol Plant 85: 147–156 (1992).
Christoffersen RA, Warm E, Laties GG: Gene expression during fruit ripening in avocado. Planta 155: 52–57 (1982).
Davies K, Grierson D: Identification of cDNAs clones for tomato (Lycopersicon esculentum Mill.) mRNAs that accumulate during fruit ripening and leaf senescence in response to ethylene. Planta 179: 73–80 (1989).
Goldschmidt EE, Huberman M, Goren R: Probing the role of endogenous ethylene in the degreening of citrus fruit with ethylene antagonists. Plant Growth Regul 12: 325–329 (1993).
Gray J, Picton S, Shabbeer J, Schuch W, Grierson D: Molecular biology of fruit ripening and its manipulation with antisense genes. Plant Mol Biol 19: 69–87 (1992).
Grierson D: Nucleic acid and protein synthesis during fruit ripening and senescence. In: Davies I, Sigee DC (eds) Cell Ageing and Cell Death, pp. 189–202. Cambridge University Press, Cambridge (1994).
Jones JDG, Dunsmuir P, Bedbrook J: High levels of expression of introduced chimeric genes in regenerated transformed plants. EMBO J 4: 2411–2414 (1985).
Lincoln JE, Cordes S, Read E, Fischer RL. Regulation of gene expression by ethylene during Lycopersicon esculentum (tomato) fruit development. Proc Natl Acad Sci USA 84: 2793–2797 (1987).
Lotan T, Ori N, Fluhr R: Pathogenesis-related proteins are developmentally regulated in tobacco flowers. Plant Cell 1: 881–887 (1989).
Maier UP, Brewster LC, Hsu AC: Ethylene-accelerated limonoids metabolism in Citrus fruits: a process for reducing juice bitterness. J Agric Food Chem 21: 490–495 (1973).
Maniatis T, Fritsch EF, Sambrook J: Molecular cloning: A Laboratory Manual, pp. 187–210. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1982).
Manning K: Changes in gene expression during strawberry fruit ripening and their regulation by auxin. Planta 194: 62–68 (1994).
Peñarrubia L, Moreno J, García-Martínez JL: Proteolysis of ribulose-1,5-disphosphate carboxylase/oxygenase in leaves of Citrus explants throught the annual cycle and its regulation by ethylene. Physiol Plant 73: 1–6 (1988).
Purvis AC, Barmore CR: Involvement of ethylene in Chlorophyll degradation in peel of Citrus fruit. Plant Physiol 68: 854–856 (1981).
Rasmussen GK: Cellulase activity, endogenous abscisic acid and ethylene in four Citrus cultivars during maturation. Plant Physiol 56: 765–767 (1975).
Shimokawa K, Sakanoshita A, Horiba K: Ethylene-induced changes of chloroplast structure in Satsuma mandarin (Citrus unshiu Mark). Plant Cell Physiol 19: 229–236 (1978).
Soule Y, Grierson W: Anatomy and physiology. In: Wardowski W, Nagy S, Grierson W (eds) Fresh Citrus Fruits, pp. 1–22. AVI, Westport (1986).
Stewart I, Wheaton TA. Carotenoid in citrus: their accumulation induced by ethylene. J Agric Food Chem 20: 448–449 (1972).
Theologis, A: One rotten apple spoils the whole bushel: the role of ethylene in fruit ripening. Cell: 181–184 (1992).
Velunthambi K, Poovaiah BW: Auxin regulated polypeptide changes at different stages of strawberry fruit development. Plant Physiol 75: 349–353 (1984).
Zacarias L, Tudela D, Primo-Millo E: Autoinhibition of ethylene biosynthesis in isolated citrus petals. Sci Hort 46: 55–60 (1990).
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Alonso, J.M., Chamarro, J. & Granell, A. Evidence for the involvement of ethylene in the expression of specific RNAs during maturation of the orange, a non-climacteric fruit. Plant Mol Biol 29, 385–390 (1995). https://doi.org/10.1007/BF00043661
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DOI: https://doi.org/10.1007/BF00043661