Abstract
Meristems aseptically isolated from shoots developed on sugarbeet (Beta vulgaris L.) inflorescences were precultured on modified MS agar medium containing 19.4 μM 6-benzylaminopurine, 6 μM triiodobenzoic acid, and supplemented with 5% DMSO. After two days the meristems were transferred to liquid modified MS medium and the cryoprotectants sorbitol and DMSO added in varying concentrations. The meristems were frozen to −40°C and stored in liquid nitrogen. Growth resumed when the meristems were quick-thawed at 39°C.
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Chen, THH, Kartha, KK, Constabel, F, Gusta, LV (1984) Freezing characteristics of culturedCartharanthus roseus (1) G. Don cells treated with dimethylsulfoxide and sorbitol in relation to cryopreservation. Plant Physiol 75: 720–725
Finkle, BJ, Ulrich, JM (1979) Effects of cryoprotectants in combination on the survival of frozen sugarcane cells. Plant Physiol 63: 598–604
Haskins, RH, Kartha, KK (1980) Freeze preservation of pea meristems: cell survival. Can J Bot 58: 833–840
Jarl, J, Bornman, CH (1986) Observations on genotypic variation inBeta vulgaris (sugarbeet) tissues cultured in vitro. Hereditas 105: 55–59
Kartha, KK (1982) Cryopreservation of plant meristems and cells. In: Wetter, LR, Constabel, F (Eds) Plant Tissue Culture Methods, pp 25–33. NRC Prairie Regional Lab, Saskatoon
Kartha, KK (1985) Meristem culture and germplasm preservation. In: Kartha, KK (Ed) Cryopreservation of Plant Cells and Organs, pp 115–134 CRC Press Inc, Boca Raton
Kartha, KK, Leung, NL, Gamborg, OL (1979) Freeze preservation of pea meristems in liquid nitrogen andsubsequent plant regeneration. Plant Sci Lett 15: 7–15
Kartha, KK, Leung, NL, Pahl, K (1980) Cryopreservation of strawberry meristems and mass propagation of plantlets. J Amer Soc Hort Sci 105 (4): 481–484
Murashige, T, Skoog, F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497
Remick IAH (1987) Regeneration of plantlets from undifferentiated ovules of sugarbeet in vitro. (Unpublished thesis). Botany Department, North Dakota State University, Fargo, North Dakota
Sakai, A (1985) Cryopreservation of shoot-tips of fruit trees and herbaceous plants. In Karth, KK (Ed) cryopreservation of Plant Cells and Organs, pp 135–158. CRC Press Inc, Boca Raton
Steponkus, PL (1985) Fundamental aspects of cryoinjury as related to cryopreservation of plant cells and organs. In: Zaitlin, Day, Hollaender (Eds) Biotechnology in Plant Science, pp 145–159. Academic Press, New York
Withers, LA (1985) Cryopreservation of cultured cells and meristems. In: Vasil, IK (Ed) Cell Culture and Somatic Cell Genetics of Plants. Vol 2, pp 253–316. Academic Press, New York
Withers, LA (1985) Cryopreservation of cultured plant cells and protoplasts. In: Kartha, KK (Ed) Cryopreservation of Plant Cells and Organs, pp 243–267. CRC Press Inc, Boca Raton
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Braun, A. Cryopreservation of sugarbeet germplasm. Plant Cell Tiss Organ Cult 14, 161–168 (1988). https://doi.org/10.1007/BF00043406
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DOI: https://doi.org/10.1007/BF00043406