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Evolution of PS IIα and PS IIβ centers during the greening of Euglena gracilis Z: Correlations with changes in lipid content

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Abstract

The building up of the two types of reaction centers, PS IIα and PS IIβ, was investigated during the greening of Euglena gracilis Z cells in resting medium. The maximal values in the proportion of PS IIα centers (55%) and in the oxygen evolved per chlorophyll were reached at the outbreak of greening, when accumulation of galactolipids (MGDG and DGDG) rich in unsaturated fatty acids occurred, and when anionic lipids (SQDG and PG) emerged. As the greening progressed, the chlorophyll accumulation corresponded to a secondary enrichment in PS IIβ centers, which built up more rapidly than PS IIα centers; correlatively, a general saturation of the fatty acids constitutive of all lipid classes took place.

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Abbreviations

DCMU:

3-(3,4-dichlorophenyl)-1,1-dimethylurea

DGDG:

digalactosyldiacylglycerol

FAME:

Tatty acid methyl esters

HEPES:

acide (N-[2-hydroxyethyl]piperazine-N′-[2-ethane sulfonic]

MGDG:

monogalactosyldiacylglycerol

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PG:

phosphatidylglycerol

PQ:

plastoquinone

PS I:

Photosystem I

PS II:

Photosystem II

QA :

primary quinone electron acceptor of PS II

QB :

secondary quinone electron acceptor of PS II

SQDG:

sulfoquinovosyldiacylglycerol

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Authors and Affiliations

  1. Laboratoire des Membranes Biologiques, Université Paris VII, Tour 54-53, 2 Place Jussieu, 75005, Paris, France

    Mimoun El Kaoua

  2. Groupe de Biochimie et Biologie Moléculaire Végétales, E.A. 917, UFR Sciences, Université d'Angers, 2 Boulevard Lavoisier, 49045, Angers Cedex, France

    Danielle L. Laval-Martin

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  1. Mimoun El Kaoua
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  2. Danielle L. Laval-Martin
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Kaoua, M.E., Laval-Martin, D.L. Evolution of PS IIα and PS IIβ centers during the greening of Euglena gracilis Z: Correlations with changes in lipid content. Photosynth Res 43, 155–163 (1995). https://doi.org/10.1007/BF00042973

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  • DOI: https://doi.org/10.1007/BF00042973

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