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Influence of dicamba and casein hydrolysate on somatic embryo number and culture quality in cell suspensions of Dactylis glomerata (Gramineae)

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Abstract

The effects of various concentrations and combinations of dicamba (3,6-dichloro-o-anisic acid) and casein hydrolysate on growth, mucilage accumulation, somatic embryo and root development in suspension cultures of Dactylis glomerata L. (orchardgrass) were examined. Fresh weight of culture tissue was increased with 20 μM but not with 80 or 160 μM dicamba in treatments with 1–4 g/l casein hydrolysate. Different casein hydrolysate concentrations did not alter the amount of mucilage (measured by viscosity) in the supernatant in the absence of dicamba. However, the addition of dicamba increased viscosity with 80 μM giving the maximum response. Casein hydrolysate produced the greatest viscosity at 1–3 g/l in treatments where dicamba was present. Both dicamba and casein hydrolysate were required for development of somatic embryos. Dicamba at 40 μM with 3–4 g/l casein hydrolysate produced approximately 2000 embryos/35 ml of suspension. Root development was inhibited by dicamba and stimulated by the presence of casein hydrolysate. The usefulness of medium component manipulations for influencing somatic embryogenesis and culture quality is discussed.

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References

  1. Bayley JM, King J, Gamborg OL (1972) The ability of amino compounds and conditioned medium to alleviate the reduced nitrogen requirement of soybean cells grown in suspension cultures. Planta 105: 25–32

    Google Scholar 

  2. Behrend J, Mateles RI (1975) Nitrogen metabolism in plant cell suspension cultures I. Effect of amino acids on growth. Plant Physiol 56: 584–589

    Google Scholar 

  3. Bligny R (1977) Growth of suspension-cultured Acer pseudoplatanus L. cells in automatic culture units of large volume. Plant Physiol 59: 502–505

    Google Scholar 

  4. Botti C, Vasil IL (1983) Plant regeneration by somatic embryogenesis from parts of cultured mature embryos of Pennisetum americanum (L.) K. Schum. Z Pflanzenphysiol 111: 319–325

    Google Scholar 

  5. Chang YF (1983) Plant regeneration in vitro from leaf tissues derived from cultured immature embryos of Zea mays L. Plant Cell Reports 2: 183–185

    Google Scholar 

  6. Conger BV (1981) Agronomic Crops. In: Conger BV (ed), Cloning agricultural plant via in vitro techniques, pp 165–215, CRC Press, Boca Raton, FL

    Google Scholar 

  7. Conger BV, Gray DJ (1984) In vitro culture in forage grass improvement. Proc 28th Grass Breeders Work Planning Conf (in press)

  8. Conger BV, Hanning GE, Gray DJ, McDaniel JK (1983) Direct embryogenesis from mesophyll cells of orchardgrass. Science 221: 850–851

    Google Scholar 

  9. Conrad PA, Binari LLW, Racusen RH (1982) Rapidly-secreting cultured oat cells serve as a model system for the study of cellular exocytosis. Characterization of cells and isolated secretory vesicles. Protoplasma 112: 196–204

    Google Scholar 

  10. Cure WW, Mott RL (1978) A comparative anatomical study of organogenesis in cultured tissues of maize, wheat, and oats. Physiol Plant 42: 91–96

    Google Scholar 

  11. Dale PJ, Thomas E, Brettell RIS, Wernicke W (1981) Embryogenesis from cultured immature inflorescence and nodes of Lolium multiflorum. Plant Cell Tissue Organ Culture 1: 47–55

    Google Scholar 

  12. Gamborg OL (1970) The effects of amino acids and ammonium on the growth of plant cells in suspension culture. Plant Physiol 45: 372–375

    Google Scholar 

  13. Genovesi AD, Collins GB (1982) In vitro production of haploid plants of corn via anther culture. Crop Sci 22: 1137–1144

    Google Scholar 

  14. Gray DJ, Conger BV (1984) Nonzygotic embryogenesis in tissue cultures of forage grasses. Proc 40th Southern Pasture and Forage Crop Improvement Conf (in press)

  15. Gray DJ, Conger BV, Hanning GE (1984) Somatic embryogenesis in suspension and suspension-derived callus cultures of Dactylis glomerata. Protoplasma 122: 196–202

    Google Scholar 

  16. Green CE, Armstrong CL, Anderson PC (1983) Somatic cell genetic systems in corn. In: Fazelahmad A, Downey K, Schultz J, Voellmy RW (eds) Advances in gene technology: molecular genetics of plants and animals. Miami Winter Symposium Series Vol 20, Academic Press, NY

    Google Scholar 

  17. Ho WJ, Vasil IK (1983) Somatic embryogenesis in sugarcane (Saccharum officinarum L.): Growth and plant regeneration from embryogenic cell suspension cultures. Ann Bot 51: 719–726

    Google Scholar 

  18. King PJ, Potrykus I, Thomas E (1978) In vitro genetics of cereals: problems and perspectives. Physiol Veg 16: 381–399

    Google Scholar 

  19. Lu CY, Vasil IK (1981) Somatic embryogenesis and plant regeneration from freely-suspended cells and cell groups of Panicum maximum Jacq. Ann Bot 48: 543–548

    Google Scholar 

  20. Nakano H, Maeda E (1974) Histology of development and root differentiation in rice callus. Proc Crop Sci Soc Japan 43: 345–353

    Google Scholar 

  21. Ozias-Akins P, Vasil IK (1983) Proliferation of and plant regeneration from the epiblast of Triticum aestivum (wheat; Gramineae) embryos. Amer J Bot 70: 1092–1097

    Google Scholar 

  22. Ozias-Akins P, Vasil IK (1983) Improved efficiency and normalization of somatic embryogenesis in Triticum aestivum (wheat). Protoplasma 117: 40–44

    Google Scholar 

  23. Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50: 199–204

    Google Scholar 

  24. Stuart DA, Strickland SG (1984) Somatic embryogenesis from cell cultures of Medicago sativa L. I. The role of amino acid additions to the regeneration medium. Plant Sci Lett 34: 165–174

    Google Scholar 

  25. Tanaka H, Nishijima F, Suwa M, Iwamoto T (1983) Rotating drum fermentor for plant cell suspension cultures. Biotechnology and Bioengineering 25: 2359–2370

    Google Scholar 

  26. Tisserat B, Esan EB, Murashige T (1978) Somatic embryogenesis in angiosperms. Hort Rev 1: 1–78

    Google Scholar 

  27. Truong-Andre I, Demarly Y (1984) Obtaining plants by in vitro culture of unfertilized maize ovaries (Zea mays L.) and preliminary studies on the progeny of a gynogenetic plant. Z Pflanzenzuchtg 92: 309–320

    Google Scholar 

  28. Vasil IK (1982) Plant cell culture and somatic cell genetics of cereals and grasses. In: Vasil IK, Scowcroft WR, Frey KJ (eds) Plant improvement and somatic cell genetics, pp 179–203, Academic Press, N.Y.

    Google Scholar 

  29. Vasil V, Vasil IK (1981) Somatic embryogenesis and plant regeneration from suspension cultures of pearl millet (Pennisetum americanum). Ann Bot 47: 669–678

    Google Scholar 

  30. Vasil V, Vasil IK (1982) Characterization of an embryogenic cell suspension culture derived from cultured inflorescences of Pennisetum americanum (pearl millet, Gramineae). Amer J Bot 69: 1441–1449

    Google Scholar 

  31. Wernicke W, Brettell R, Wakizuka T, Potrykus I (1981) Adventitious embryoid and root formation from rice leaves. Z Pflanzenphysiol 103: 361–365

    Google Scholar 

  32. Wernicke W, Potrykus I, Thomas E (1982) Morphogenesis from cultured leaf tissue of Sorghum bicolor — the morphogenetic pathways. Protoplasma 111: 53–62

    Google Scholar 

  33. Wetherell DF, Dougall DK (1976) Sources of nitrogen supporting growth and embryogenesis in cultured wild carrot tissue. Physiol Plant 37: 97–103

    Google Scholar 

  34. Woo SC, Huang CY (1980) Anther Culture of Oryza glaberrima Steud. and its hybrid with Oryza sativa L. Bot Bull Acad Sinica 21: 75–79

    Google Scholar 

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Author notes

  1. D. J. Gray

    Present address: Agricultural Research and Education Center, University of Florida, IFAS, P.O. Box 388, 32749-0388, Leesburg, FL, USA

Authors and Affiliations

  1. Department of Plant and Soil Science, University of Tennessee, 37901-1071, Knoxville, TN, USA

    D. J. Gray & B. V. Conger

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  1. D. J. Gray
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  2. B. V. Conger
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Gray, D.J., Conger, B.V. Influence of dicamba and casein hydrolysate on somatic embryo number and culture quality in cell suspensions of Dactylis glomerata (Gramineae). Plant Cell Tiss Organ Cult 4, 123–133 (1985). https://doi.org/10.1007/BF00042270

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  • DOI: https://doi.org/10.1007/BF00042270

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