Abstract
A tissue culture method is described for clonal multiplication of Leucaena leucocephala K67 using single lateral bud explants from 2–3 m tall greenhouse grown trees. N-6 benzyladenine (BA: 3.0 mg.1-1) and napthaleneacetic acid (NAA: 0.05 mg.1-1) in Murashige & Skoog's (MS) medium were found to be best suited for multiple shoot differentiation in 4–5 week old cultures. Analysis of variance of the main treatment effects of BA and NAA on shoot parameters showed that BA significantly (P=0.001) affected shoot development while NAA did not. A shoot multiplication rate of 22±3.63 shoots per bud explant was obtained in 150 days on 1/2 strength MS medium with 3.0 mg.1-1 BA and 0.05 mg.1-1 NAA. Shoots developed adventitious roots within 15 days in 1/2 strength MS medium containing indole-3-butyric acid (IBA: 3.0 mg.1-1) and Kinetin (0.05 mg.1-1). Eighty percent of the transplanted plantlets are being grown in greenhouse conditions.
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References
Brewbaker JL, Hutton EM (1979) Leucaena-versatile tropical legume. New Agricultural Crops, American Association for Advancement of Science. G.A.Ritchie, Ed. Westview Press, Boulder, CO 207–259pp.
Brewbaker JL (1982) Systematics, self-incompatibility, breeding systems and genetic improvement of Leucaena species. In Leucaena research in the Asian-Pacific Region. Proceedings of workshop in Singapore No. 1982. International Development Research Center Ottawa Publishers 17–22 pp.
Glovack L, Greatbatch W (1980) Successful tissue culture of Leucaena. Leucaena Research Reports 3:81. A publication of the nitrogen fixing tree association (NFTA) P.O. Box 680, Waimanalo, Hawaii, U.S.A.
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assay with tobacco tissue culture. Physiol. Plant. 15, 473–497.
Murashige T (1974) Plant propagation through tissue cultures. Ann. Rev. Plant Physiol. 25: 135–166.
Nagmani R, Venketeswaran S (1982) Morphogenetic responses of cultured hypocotyl and cotyledonary segments of Leucaena. 34th Annual Meeting of the tissue culture association, In Vitro abstract: 265.
Nagmani R, Venketeswaran S (1983) In vitro culture of hypocotyl and cotyledon segments of Leucaena. Leucaena Research Reports 4: 88–89 NFTA, Hawaii, U.S.A.
Peasley EL, Collins GB (1980) Development of an in vitro culture system for Leucaena K8. Leucaena News Letter 1: 54. NFTA, Hawaii, U.S.A.
Ravishankar GA, Wali A, Grewal S (1983) Plantlet formation through tissue cultures of Leucaena leucocephala. Leucaena Research Reports 4: 37 NFTA, Hawaii, U.S.A.
Reyes I (1983) Evaluation of Leucaena forage and biofuel potential in semi-arid South Texas. M.S. Thesis, Texas A & I University, Kingsville, TS, U.S.A. 96 pages.
Venketeswaran S, Gandhi V (1982) Mass propagation and genetic improvement of forest trees for biomass production by tissue culture. Biomass 2: 5–15.
Vietmeyer N, Cotton B, Ruskin FR, (1977) Leucaena promising forage and tree crop for the tropics. U.S. National Academy of Science. Washington, D.C. 80 pp.
White PR (1934) Potentially unlimited growth of excised tomato root tips in a liquid medium. Plant Physiol 9, 585–600.
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Goyal, Y., Bingham, R.L. & Felker, P. Propagation of the tropical tree, Leucaena leucocephala K67, by in vitro bud culture. Plant Cell Tiss Organ Cult 4, 3–10 (1985). https://doi.org/10.1007/BF00041650
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DOI: https://doi.org/10.1007/BF00041650