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Cloning and expression of a plant homologue of the small subunit of the Golgi-associated clathrin assembly protein AP19 from Camptotheca acuminata

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Abstract

Clathrin-coated vesicles (CCVs) are involved in selective protein transport in eukaryotes. AP-1 and AP-2 are protein complexes found in the CCVs of the Golgi apparatus and the plasma membrane respectively. AP19 is the smallest polypeptide chain components of AP-1. We have identified a cDNA clone (CAP19) encoding a putative homologue for the assembly protein AP19 from the Chinese medicinal tree, Camptotheca acuminata. The deduced polypeptide contains 161 amino acids and has a predicted M r of 18 820. DNA blot analysis suggests that the AP19s of C. acuminata are encoded by a small gene family. CAP19 was expressed ubiquitously throughout the plant suggesting that it may be involved in general Golgi-mediated secretion.

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  1. Department of Biology, Texas A&M University, 77843-3258, College Station, TX, USA

    Ignacio E. Maldonado-Mendoza & Craig L. Nessler

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  1. Ignacio E. Maldonado-Mendoza
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  2. Craig L. Nessler
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Maldonado-Mendoza, I.E., Nessler, C.L. Cloning and expression of a plant homologue of the small subunit of the Golgi-associated clathrin assembly protein AP19 from Camptotheca acuminata . Plant Mol Biol 32, 1149–1153 (1996). https://doi.org/10.1007/BF00041398

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  • DOI: https://doi.org/10.1007/BF00041398

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