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In vitro culture of shoots of Pinus caribaea on a liquid medium

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Abstract

Shoot apices of a clone of Pinus caribaea Morelet were cultured and multiplied in vitro by supporting them with their basal cut ends immersed in a liquid nutrient medium.

The initial heights of explants and their initial numbers of leaves were positively correlated with the numbers of buds and shoots produced by the explants after a bud induction phase and after a shoot elongation phase. The final numbers of buds and shoots were positively correlated with reductions in the quantities of phosphorus detected in the media and negatively correlated with the numbers of brown leaves produced on the explants.

In a comparison between the growth of shoot explants on liquid and solid media, shoots incubated on the liquid medium showed significantly greater increases in length in a four-week period than those cultured on solid medium.

This technique, using liquid media, provides a system in which both the nutrient utilization and the growth rates of isolated pine tissues can be readily assessed. Furthermore, the multiplication rate of the tissue can be predicted following the observation of correlated characters early in the micropropagation cycle.

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Authors and Affiliations

  1. Department of Applied Biology, University of Cambridge, Pembroke Street, CB2 3DX, Cambridge, UK

    D. I. Skidmore, A. J. Simons & Seema Bedi

Authors
  1. D. I. Skidmore
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  2. A. J. Simons
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  3. Seema Bedi
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Skidmore, D.I., Simons, A.J. & Bedi, S. In vitro culture of shoots of Pinus caribaea on a liquid medium. Plant Cell Tiss Organ Cult 14, 129–136 (1988). https://doi.org/10.1007/BF00041186

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  • DOI: https://doi.org/10.1007/BF00041186

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