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Cloning and characterization of differentially expressed genes in imbibed dormant and afterripened Avena fatua embryos

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Abstract

To analyze the patterns of gene expression associated with seed dormancy in wild oat (Avena fatua), we have isolated cDNA clones corresponding to genes that are differentially expressed in dormant and afterripened line M73 embryos. Gene transcripts of these clones were maintained in embryos of imbibed dormant caryopses, but declined rapidly in afterripened embryos after imbibition. GA3 treatment of dormant caryopses, which breaks dormancy, could lower the transcript levels in dormant embryos. When the germination of afterripened caryopses was inhibited by high temperature (35 °C), the decline in abundance of the transcripts in afterripened embryos was arrested. These genes were expressed to various degrees in water-stressed, but not in unstressed, 7-day-old seedlings. The expression of the genes was also ABA-inducible in afterripened embryos. The expression patterns in non-dormant line SH430 wild oat were similar to those of afterripened M73. DNA sequence analyses indicated that some of the cDNA clones encode LEA (late embryogenesis-abundant) proteins and aldose reductase. The significance of the expression of these genes in maintaining seed dormancy or longevity is discussed.

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References

  1. Adkins SW, Loewen M, Symons SJ: Variation within pure line of wild oat (Avena fatua) in relation to degree of primary dormancy. Weed Sci 34: 859–864 (1986).

    Google Scholar 

  2. Baker HG: The evolution of weeds. Annu Rev Ecol System 5: 1–24 (1974).

    Google Scholar 

  3. Banting JD: Studies on the persistence of Avena fatua. Can J Plant Sci 46: 129–140 (1966).

    Google Scholar 

  4. Bartels D, Engelhardt K, Roncarati R, Schneider K, Rotter M, Salamini F: An ABA and GA modulated gene expressed in the barley embryo encodes an aldose reductase related protein. EMBO J 10: 1037–1043 (1991).

    Google Scholar 

  5. Bewley JD, Black M: Physiology and Biochemistry of Seeds, vol. 2, pp. 69. Springer-Verlag, Berlin (1982).

    Google Scholar 

  6. Comai L, Harada JJ: Transcriptional activities in dry seed nuclei indicate the timing of the transition from embryogeny to germination. Proc Natl Acad Sci USA 87: 2671–2674 (1990).

    Google Scholar 

  7. Corbineau F, Poljakoff-Mayber A, Come D: Responsiveness to abscisic acid of embryos of dormant oat (Avena sativa) seeds. Involvement of ABA-inducible proteins. Physiol Plant 83: 1–6 (1991).

    Google Scholar 

  8. DureIII L: A repeating 11-mer amino acid motif and plant desiccation. Plant J 3: 363–369 (1993).

    Google Scholar 

  9. DureIII L, Crouch M, Harada J, Ho T-H D, Mundy J, Quatrano R, Thomas T, Sung ZR: Common amino acid sequence domains among the LEA proteins of higher plants. Plant Mol Biol 12: 475–486 (1989).

    Google Scholar 

  10. Dyer WE: Dormancy-associated embryonic mRNAs and proteins in imbibed Avena fatua caryopses. Physiol Plant 88: 201–211 (1993).

    Google Scholar 

  11. Foley ME: The effect of wounding on primary dormancy in wild oat (Avena fatua) caryopses. Weed Sci 35: 180–184 (1987).

    Google Scholar 

  12. Foley ME: Effect of soluble sugars and gibberellic acid in breaking dormancy of excised wild oat (Avena fatua) embryos. Weed Sci 40: 2080214 (1992).

    Google Scholar 

  13. Foley ME, Nichols MB, Myers SP: Carbohydrate concentrations and interactions in afterripening-responsive dormant Avena fatua caryopses induced to germinate by gibberellic acid. Seed Sci Res 3: 271–278 (1993).

    Google Scholar 

  14. Galau GA, Jakobsen KS, Hughes DW: The control of late dicot embryogenesis and early germination. Physiol Plant 81: 280–288 (1991).

    Google Scholar 

  15. Goldmark PJ, Curry J, Morris CF, Walker-Simmons MK: Cloning and expression of an embryo-specific mRNA up-regulated in hydrated dormant seeds. Plant Mol Biol 19: 433–441 (1992).

    Google Scholar 

  16. Hance BA, Bevington JM: Changes in protein synthesis during stratification and dormancy release in embryos of sugar maple (Acer saccharum). Physiol Plant 86: 365–371 (1992).

    Google Scholar 

  17. Hole DJ, Smith JD, Cobb BG: Regulation of embryo dormancy by manipulation of abscisic acid in kernels and associated cob tissue of Zea mays L. cultured in vitro. Plant Physiol 91: 101–105 (1989).

    Google Scholar 

  18. Hong B, Barg R, Ho T-HD: Developmental and organ-specific expression of an ABA-and stress-induced protein in barley. Plant Mol Biol 18: 663–674 (1992).

    Google Scholar 

  19. Hughes DW, Galau GA: Translation efficiency of Lea mRNAs in cotton embryos: minor changes during embryogenesis and germination. Plant Mol Biol 9: 301–313 (1987).

    Google Scholar 

  20. Hughes DW, Galau GA: Temporally modular gene expression during cotyledon development. Genes Devel 3: 358–369 (1989).

    Google Scholar 

  21. Hughes DW, Galau GA: Developmental and environmental induction of Lea and LeaA mRNAs and the post-abscission program during embryo culture. Plant Cell 3: 605–618 (1991).

    Google Scholar 

  22. Jana S, Acharya SN, Naylor JM: Dormancy studies in seed of Avena fatua. 10. On the inheritance of germination behaviour. Can J Bot 57: 1663–1667 (1979).

    Google Scholar 

  23. Jana S, Naylor JM: Dormancy studies in seed of Avena fatua. 11. Heritability for seed dormancy. Can J Bot 58: 91–93 (1980).

    Google Scholar 

  24. Karssen CM, Brinkhorst-van der Swan DLC, Breekland AE, Koornneef M: Induction of dormancy during seed development by endogenous abscisic acid: studies on abscisic acid deficient genotypes of Arabidopsis thaliana (L.) Heynh. Planta 157: 158–165 (1983).

    Google Scholar 

  25. Karssen CM, Lacka E: A revision of the hormone balance theory of seed dormancy: Studies on gibberellin and/or abscisic acid-deficient mutants of Arabidopsis thaliana. In: Bopp M (ed) Plant Growth Substance 1985, pp. 315–323. Springer-Verlag, Berlin (1986).

    Google Scholar 

  26. Kawakami N, Kawabata C, Noda K: Differential changes in levels of mRNAs during maturation of wheat seeds that are susceptible and resistant to preharvest-sprouting. Plant Cell Physiol 33: 511–517 (1992).

    Google Scholar 

  27. Lee SP, Chen THH: Molecular cloning of abscisic acid-responsive mRNAs expressed during the induction of freezing tolerance in bromegrass (Bromus inermis Leyss) suspension culture. Plant Physiol 101: 1089–1096 (1993).

    Google Scholar 

  28. Li B, Foley ME: Differential polypeptide patterns in imbibed dormant and afterripened Avena fatua embryos. J Exp Bot 45: 275–279 (1994).

    Google Scholar 

  29. Litts JC, Colewell GW, Chakerian RL, Quatrano RS: The nucleotide sequence of a cDNA encoding the wheat Em protein. Nucl Acids Res 15: 3607–3618 (1987).

    Google Scholar 

  30. Love J, Deininger P: Hybridization selections, using immobilized driver DNA, to enrich for clones unique to a library. Bio Techniques 11: 88–92 (1991).

    Google Scholar 

  31. Morris CF, Anderberg RJ, Goldmark PJ, Walker-Simmons MK: Molecular cloning and expression of abscisic acid-responsive genes in embryos of dormant wheat seeds. Plant Physiol 95: 814–821 (1991).

    Google Scholar 

  32. Naylor JM, Simpson GM: Dormancy studies in seed of Avena fatua. 2. A gibberellin-sensitive inhibitory mechanism in the embryo. Can J Bot 39: 281–295 (1961).

    Google Scholar 

  33. Ried JL, Walker-Simmons MK: Synthesis of abscisic acid-responsive, heat-stable proteins in embryonic axes of dormant wheat grain. Plant Physiol 93: 662–667 (1990).

    Google Scholar 

  34. Roberts JK, DeSimone NA, Lingle WL, DureIII L: Cellular concentrations and uniformity of cell-type accumulation of two Lea proteins in cotton embryos. Plant Cell 5: 569–580 (1993).

    Google Scholar 

  35. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).

    Google Scholar 

  36. Simpson GM: Seed Dormancy in Grasses. Cambridge University Press, Cambridge, UK (1990).

    Google Scholar 

  37. Symons SJ, Simpson GM, Adkins SW: Secondary dormancy in Avena fatua: effect of temperature and afterripening. Physiol Plant 70: 419–426 (1987).

    Google Scholar 

  38. Tomlinson DR, Stevens EJ, Diemel LT: Aldose reductase inhibitors and their potential for the treatment of diabetic complications. Trends Pharmacol Sci 15: 293–297 (1994).

    Google Scholar 

  39. Tuan DYH, Bonner J: Dormancy associated with repression of genetic activity. Plant Physiol 39: 768–772 (1964).

    Google Scholar 

  40. Walker-Simmons M: ABA levels and sensitivity in developing wheat embryos of sprouting resistant and susceptible cultivars. Plant Physiol 84: 61–66 (1987).

    Google Scholar 

  41. Zorner PS, Zimdahl RL, Schweizer EE: Sources of viable seed loss in buried dormant and non-dormant populations of wild oat (Avena fatua L.) seed in Colorado. Weed Res 24: 143–150 (1984).

    Google Scholar 

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  1. Bailin Li

    Present address: Department of Agronomy, Purdue University, 47907-1150, West Lafayette, IN, USA

Authors and Affiliations

  1. Department of Botany & Plant Pathology, Purdue University, 47907-1155, West Lafayette, IN, USA

    Bailin Li & Michael E. Foley

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  1. Bailin Li
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  2. Michael E. Foley
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Li, B., Foley, M.E. Cloning and characterization of differentially expressed genes in imbibed dormant and afterripened Avena fatua embryos. Plant Mol Biol 29, 823–831 (1995). https://doi.org/10.1007/BF00041171

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  • DOI: https://doi.org/10.1007/BF00041171

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