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Light-inducible geneHSP70B encodes a chloroplast-localized heat shock protein inChlamydomonas reinhardtii

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Abstract

The nuclear heat shock geneHSP70B ofChlamydomonas reinhardtii is inducible by heat stress and light. Induction by either environmental cue resulted in a transient elevation in HSP70B protein. Here we describe the organization and nucleotide sequence of theHSP70B gene. The deduced protein exhibits a distinctly higher homology to prokaryotic HSP70s than to those of eukaryotes, including the cytosolic HSP70A ofChlamydomonas reinhardtii. The HSP70B protein, as previously demonstrated by in vitro translation, is synthesized with a cleavable presequence. Using an HSP70B-specific antibody, this heat shock protein was localized to the chloroplast by cell fractionation experiments. A stromal location was suggested by the presence of a conserved sequence motif used for cleavage of presequences by a signal peptidase of the stroma. Amino acid alignments of HSP70 proteins from various organisms and different cellular compartments allowed the identification of sequence motifs, which are diagnostic for HSP70s of chloroplasts and cyanobacteria.

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  1. Institut für Biologie III, Universität Freiburg, Schänzlestrasse 1, D-79104, Freiburg, Germany

    Christian Drzymalla, Michael Schroda & Christoph F. Beck

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  1. Christian Drzymalla
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  2. Michael Schroda
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  3. Christoph F. Beck
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Drzymalla, C., Schroda, M. & Beck, C.F. Light-inducible geneHSP70B encodes a chloroplast-localized heat shock protein inChlamydomonas reinhardtii . Plant Mol Biol 31, 1185–1194 (1996). https://doi.org/10.1007/BF00040835

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  • DOI: https://doi.org/10.1007/BF00040835

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