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The structure and biogenesis of plant oil bodies: the role of the ER membrane and the oleosin class of proteins

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References

  1. Aalen RB: The transcripts encoding two oleosin isoforms are both present in the aleurone and in the embryo of barley (Hordeum vulgare L.) seeds. Plant Mol Biol 28: 583–588 (1995).

    PubMed  Google Scholar 

  2. Banfield DK, Lewis MJ, Pelham HRB: A SNARE-like protein required for traffic through the Golgi complex. Nature 375: 806–809 (1995).

    Article  PubMed  Google Scholar 

  3. Bassham DC, Gal S, da Silva Conceicao A, Raikhel NV: AnArabidopsis syntaxin homologue isolated by functional complementation of a yeastpep 12 mutant. Proc Natl Acad Sci USA 92: 7262–7266 (1995).

    PubMed  Google Scholar 

  4. Batchelder C, Ross JHE, Murphy DJ: Synthesis and targeting ofBrassica napus oleosin in transgenic tobacco. Plant Sci 104: 39–47 (1994).

    Article  Google Scholar 

  5. Bednarek SY, Raikhel NV: Intracellular trafficking of secretory proteins. Plant Mol Biol 20: 133–150 (1992).

    PubMed  Google Scholar 

  6. Bennett MK: SNAREs and the specificity of transport versicle targeting. Curr Opin Cell Biol 7: 581–586 (1995).

    Article  PubMed  Google Scholar 

  7. Bergfield R, Hong YN, Kuhnl T, Schopfer P: Formation of oleosomes (storage lipid bodies) during embryogenesis and their breakdown during seedling growth. Planta 143: 297–303 (1978).

    Google Scholar 

  8. Chrispeels MJ, Raikhel NV: Short peptide domains target proteins to plant vacuoles. Cell 68: 613–616 (1995).

    Article  Google Scholar 

  9. Cummins I, Hills MJ, Ross JHE, Hobbs DH, Watson MD, Murphy DJ: Differential, temporal and spatial expression of genes involved in storage oil and oleosin accumulation in developing rapeseed embryo: implications for the role of oleosins and the mechanisms of oil-body formation. Plant Mol Biol 23: 1015–1027 (1993).

    PubMed  Google Scholar 

  10. Cummins I, Murphy DJ: cDNA sequence of a sunflower oleosin and transcript tissue-specificity. Plant Mol Biol 19: 873–876 (1992).

    Article  PubMed  Google Scholar 

  11. Frey-Wyssling A, Grieshaber E, Muhlethaler K: Origin of spherosomes in plant cells. J Ultrastruc Res 8: 506–516 (1963).

    Google Scholar 

  12. Finkelstein RR, Somerville CR: Three classes of abscisic acid (ABA)-insensitive mutations ofArabidopsis define genes that control overlapping subsets of ABA responses. Plant Physiol 94: 1172–1179 (1990).

    Google Scholar 

  13. Gorlich D, Hartmann E, Prehn S, Rapoport TA: A protein of the endoplasmic reticulum involved early in polypeptide translocation. Nature 357: 47–52 (1992).

    PubMed  Google Scholar 

  14. Hardwick KG, Pelham HRB: SED6 is identical ERG6, and encodes a putative methyl transferase required for ergosterol synthesis. Yeast 10: 265–269 (1994).

    PubMed  Google Scholar 

  15. Herman EM: Immunogol-localization and synthesis of an oilbody membrane protein in developing soybean seeds. Planta 172: 336–345 (1987).

    Google Scholar 

  16. Herman EM: Cell and molecular biology of seed oil development. In: Kigel J, Galili G (eds) Seed Development and Germination, kpp. 195–214 Marcel Dekker. New York (1995).

    Google Scholar 

  17. High S, Dobberstein B: Mechanisms that determine the transmembrane disposition of proteins. Curr Opin Cell Biol 4: 581–586 (1992).

    PubMed  Google Scholar 

  18. High S, Flint N, Dobberstein B: Requirements for the membrane insertion of signal-anchor type proteins. J Cell Biol. 113: 25–34 (1993).

    Google Scholar 

  19. High S, Martoglio B, Gorlich D, Andersen SSL, Ashford AJ, Giner A, Hartmann E, Prehn S, Rapoport TA, Dobberstein B, Brunner J: Site-specific photocross-linking reveals that Sec61 p and TRAM contact different regions of a membrane-inserted signal sequence. J Biol Chem 268: 26745–26751 (1993).

    PubMed  Google Scholar 

  20. Hills MJ, Watson MD, Murphy DJ: Targeting of oleosing to the oil bodies of oilseed rape (Brassica napus L.). Planta 189: 24–29 (1993).

    Article  PubMed  Google Scholar 

  21. Huang AHC: Oil bodies and oleosins in seeds. Annu Rev Plant Physiol Plant Mol Biol 43: 177–200 (1992).

    Article  Google Scholar 

  22. Hurtley SM: Membrane proteins involved in targeted membrane fusion. Trends Biochem Sci 18: 453–455 (1993).

    Article  PubMed  Google Scholar 

  23. Jacks TJ, Hensarling TP, Neucere JN, Yatsu LY, Barker RH: Isolation and physicochemical characterization of the half-unit membranes of oilseed lipid bodies. J Am Oil chem Soc 67: 353–361 (1990).

    Google Scholar 

  24. Johnson AE: Protein translocation across the ER membrane: a fluorescent light a the end of the tunnel. Trends Biochem Sci 18: 456–458 (1993).

    Article  PubMed  Google Scholar 

  25. Kalinski A, Weisemann JM, Matthews BF, Herman EM: Molecular cloning of a protein associated with soybean seed oil bodies that is similar to thiol proteases of the papain family. J Biol Chem 265: 13843–13848 (1990).

    PubMed  Google Scholar 

  26. Kalinski A, Melroy DL, Dwivedi RS, Herman EM: A soybean vacuolar protein (p34) related to thiol proteases is synthesized as a glycoprotein precursor during seed maturation. J Biol Chem 267: 12068–12076 (1992).

    PubMed  Google Scholar 

  27. Keddie JS, Hubner G, Slocombe SP, Jarvis RP, Cummins I, Edwards E-W, Shaw CH, Murphy DJ: Cloning and characterisation of an oleosin gene fromBrassica napus. Plant Mol Biol 19: 443–453 (1992).

    Google Scholar 

  28. Kutay U, Hartmann E, Rapoport TA: A class of membrane proteins with a C-terminal anchor. Trends Cell Biol 3: 72–75 (1993).

    Article  PubMed  Google Scholar 

  29. Lacey DJ, Hills MJ: Identification of a low density membrane fraction involved in storage triacylglycerol synthesis. In: Kader I-C, Mazliak P (eds) Plant Lipid Metabolism. pp. 482–484 Kluwer Academic Publishers, Dordrecht (1995).

    Google Scholar 

  30. Leber R, Zinser E, Zellnig G, Paltauf F, Daum G: Characterization of lipid particles of the yeastSaccharomyces cerevisae. Yeast 10: 1421–1428 (1994).

    PubMed  Google Scholar 

  31. Lee K, Bih FY, Learn GH, Ting JTL, Sellers C, Huang AHC: Oleosins in the gametophytes ofPinus andBrassica and their Phylogenetic relationship with those in the sporophytes of various species. Planta 193: 461–469 (1994).

    Article  PubMed  Google Scholar 

  32. Lee WS, Tzen JTC, Kridl JC, Radke SE, Huang AHC: Maize oleosin is correctly targeted to seed oil bodies inBrassica napus transformed with the maize oleosin gene. Proc Natl Acad Sci USA 88: 6181–6185 (1991).

    PubMed  Google Scholar 

  33. Li M, Keddie JS, Smith LJ, Clarke DC, Murphy DJ: Expression and characterization of the N-terminal domain of an oleosin protein from sunflower. J Biol Chem 268: 17504–17512 (1993).

    PubMed  Google Scholar 

  34. Li M, Smith LJ, Clark DC, Wilson R, Murphy DJ: Secondary structures of a new class of lipid body proteins from oilseeds. J Biol Chem 267: 8245–8253 (1992).

    PubMed  Google Scholar 

  35. Li X, Franceshi VR, Okita TW: Segregation of storage protein mRNAs on the rough endoplasmic reticulum membranes of rice endosperm cells. Cell 72: 869–879 (1993).

    Article  PubMed  Google Scholar 

  36. Loer DS, Herman EM: Cotranslational integration of soybean (glycine max) oil body membrane protein oleosin into microsomal membranes. Plant Physiol 101: 993–998 (1993).

    PubMed  Google Scholar 

  37. Lutcke H: Signal recognition particle (SRP), a ubiquitous initiator of protein translocation. Eur J Biochem 228: 531–550 (1995).

    PubMed  Google Scholar 

  38. Millichip M, Tatham AS, Jackson F, Griffiths G, Shewry PR, Stobart AK: Purification and characterization of oil bodies (oleosomes) and oil-body boundary proteins (oleosins) from the developing cotyledons of sunflower (Helianthus annuus L.). Biochem J 314: 333–337 (1996).

    PubMed  Google Scholar 

  39. Mothes W, Prehn S, Rapoport TA: Systematic probing of the environment of a translocating secretory protein during translocation through the ER membrane. EMBO J 13: 3973–3982 (1994).

    PubMed  Google Scholar 

  40. Murphy DJ: Storage lipid bodies in plants and other organisms. Prog Lipid Res 29: 299–324 (1990).

    PubMed  Google Scholar 

  41. Murphy DJ: Structure, function and biogenesis of storage lipid bodies and oleosins in plants. Prog Lipid Res 32: 247–280 (1993).

    Article  PubMed  Google Scholar 

  42. Murphy DJ: Biogenesis, function and biotechnology of plant storage lipids. Prog Lipid Res 33: 71–85 (1994).

    Article  PubMed  Google Scholar 

  43. Murphy DJ, Cummins I: Purification and immunogold localisation of the major oil body membrane protein of oilseed rape. Plant Sci 60: 47–54 (1989).

    Article  Google Scholar 

  44. Murphy DJ, Cummins I, Kang AS: Synthesis of the major oil-body membrane-protein in developing rapeseed (Brassica napus) embryos. Biochem J 258: 285–293 (1989).

    PubMed  Google Scholar 

  45. Murphy DJ, Rawsthorne S, Hills MJ: Storage lipid formation in seeds. Seed Sci Res 3: 79–95 (1993).

    Google Scholar 

  46. Parcy F, Valon C, Raynal M, Gaubier-Comella P, Delseny M, Giraudat J: Regulation of gene expression programs during Arabidopsis seed development: roles of the ABI3 locus and of endogenous abscisic acid. Plant Cell 6: 1567–1582 (1994).

    Article  PubMed  Google Scholar 

  47. Qu R, Wang SM, Lin YH, Vance VB, Huang AHC: Characterisatic and biosynthesis of membrane proteins of lipid bodies in the scutella of maize (Zea mays L.). Biochem J 234: 57–65 (1986).

    Google Scholar 

  48. Radetzky R, Langheinrich U: Induction of accumulation and degradation of the 18.4 kDa oleosin in a triacylglycerol-storing cell culture of anise (Pimpinelle anisum L.). Planta 194: 1–8 (1994).

    Article  Google Scholar 

  49. Roberts MR, Hodge R, Ross JHE, Sorenson A, Murphy DJ, Draper J, Scott R: Characterization of a new class of oleosins suggest a male gametophyte-specific lipid storage pathway. Plant J 3: 629–636 (1993).

    Article  PubMed  Google Scholar 

  50. Roberts MR, Hodge R, Scott R:Brassica napus pollen oleosins possess a characteristic C-terminal domain. Plant 195: 469–470 (1995).

    Google Scholar 

  51. Ross JHE, Murphy DJ: Differential accumulation of storage products in developing seeds and somatic cell cultures ofDaucus carota L. Plant Sci 88: 1–11 (1993).

    Article  Google Scholar 

  52. Settlage SB, Wilson RF, Kwanyuen P: Localization of diacylgycerol transferase to oil body associated endoplasmic reticulum. Plant Physiol Biochem 33: 399–407 (1995).

    Google Scholar 

  53. Shewry PR, NapierJA, Tatham AS: Seed storage proteins: structure and biosynthesis. Plant Cell: 945–956 (1995).

  54. Smith MA, Stobart AK, Shewry PR, Napier JA: tobacco cytochromeb 5: cDNA isolation, expression analysis andin vitro protein targeting. Plant Mol Biol 25: 527–537 (1994).

    PubMed  Google Scholar 

  55. Stobart AK, Stymme S, Hoglund S: Safflower microsomes catalyse oil accumulationin vitro: a model system. Planta 169: 33–37 (1986).

    Google Scholar 

  56. Thoyts PJE, Millichip MI, Stobart AK, Griffiths WT, Shewry PR, Napier JA: Expression andin vitro targeting of a sunflower oleosin. Plant Mol Biol 29: 403–410 (1995).

    PubMed  Google Scholar 

  57. Thoyts PJE, Tatham AS, Griffiths WT, Millichip MI, Stobart AK, Napier JA, Shewry PR: Characterization of a sunflower seed albumin which associates with oil bodies. Plant Sci, in press.

  58. Tzen JTC, Cao Y-z, Laurent P, Ratnayake C, Huang AHC: Lipids, proteins, and structure of seed oil bodies from diverse species. Plant Physiol 101: 267–276 (1993).

    PubMed  Google Scholar 

  59. Tzen JTC, Huang AHC: Surface structure and properties of plant seed oil bodies. J Cell Biol 117: 327–335 (1992).

    Article  PubMed  Google Scholar 

  60. Tzen JTC, Lai Y-K, Chan K-L, Huang AHC: Oleosin isoforms of high and low molecular weights are present in the oil bodies of diverse seed species. Plant Physiol 94: 1282–1289 (1990).

    Google Scholar 

  61. Tzen JTC, Lie GC, Huang AHC: Characterization of the charged components and their topology on the surface of plant seed oil bodies. J Biol Chem 267: 15626–15634 (1992).

    PubMed  Google Scholar 

  62. Vance VB, Huang AHC: The major protein from lipid bodies of maize. Characterization and structure based on cDNA cloning. J Biol Chem 262: 1476–1479 (1987).

    Google Scholar 

  63. van Rooijen GJH, Moloney MM: Plant seed oil-bodies as carriers for foreign proteins. Bio/technology 13: 72–77 (1995).

    Article  PubMed  Google Scholar 

  64. van Rooijen GJH, Moloney MM: Structural requirements of oleosin domains for subcellular targeting to the oil body. Plant physiol 109: 1353–1361 (1995).

    Article  PubMed  Google Scholar 

  65. van Rooijen GJH, Wilen RW, Holbrook LA, Moloney MM: Regulation of accumulation of mRNAs encodinga 20 kDa oil-body protein in microspore-derived embryos ofBrassica napus. Can J Bot 70: 503–508 (1992).

    Google Scholar 

  66. Walter P, Blobel G: Translocation of proteins across the endoplasmic reticulum III. Signal recognition protein (SRP) causes signal sequence-dependent and site-specific arrest of chain elongation that is released by microsomal membranes. J Cell Biol 91: 557–561 (1981).

    Article  PubMed  Google Scholar 

  67. Whifield HV, Murphy DJ, Hills MJ: Subcellular localization of very long chain fatty acid elongases in oilseeds. Phytochemistry 32: 255–258 (1993).

    Article  Google Scholar 

  68. Zou J, Abrams GD, Barton DL, Taylor DC, Pomeroy MK, Abrams SR: Induction of lipid and oleosin biosynthesis by (+)-abscisic acid and its metabolites in microspore-derived embryos ofBrassica napus L. cv Reston. Plant Physiol 108: 563–571 (1995).

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Cell Biology Department, IACR-Long Ashton Research Station, University of Bristol, Long Ashton, BS 18 9AF, Bristol, UK

    Johnathan A. Napier & Peter R. Shewry

  2. School of Biological Sciences, University of Bristol, Woodland Rd, BS 8 1UG, Bristol, UK

    A. Keith Stobart

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Napier, J.A., Stobart, A.K. & Shewry, P.R. The structure and biogenesis of plant oil bodies: the role of the ER membrane and the oleosin class of proteins. Plant Mol Biol 31, 945–956 (1996). https://doi.org/10.1007/BF00040714

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