Skip to main content
SpringerLink
Log in

The action of lipases on chloroplast membranes I. The release of plastocyanin from galactolipase-treated thylakoid membranes

  • Published:
Photosynthesis Research Aims and scope Submit manuscript

Abstract

Bean chloroplasts treated with galactolipase (lipolytic acyl hydrolase) isolated from bean leaves showed an inhibition of photosystem I activity as measured by methyl viologen-mediated oxygen uptake and NADP+ photoreduction. This inhibition was partially reversed by exogenous plastocyanin added to galactolipase-treated thylakoid membranes. Galactolipase released substantial amounts of endogenous plastocyanin (about 40%) from bean chloroplasts. The results are discussed with regard to the localization of plastocyanin in thylakoid membranes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

chlf:

chlorophyll

DCMU:

3-(2,4-dichlorophenyl)-1′,1′-dimethylurea

DGDG:

digalactosyldiacylglycerol

MGDG:

monogalactosyldiacylglycerol

MV:

methyl viologen

NADP+ :

nicotinamide dinucleotide phosphate

PC:

phosphatidylcholine

PG:

phosphatidylglycerol

PE:

phosphatidylethanolamine

PI:

phosphatidylinositol

SQDG:

sulphoquinovosyldiacylglycerol

SDS:

sodium dodecyl sulphate

TMPD:

N,N,N′,N′-tetramethyl-p-phenylenediamine

Tricine:

N-Tris-(hydroxymethyl)-methylglycine

Tris:

Tris-(hydroxymethyl)-aminomethane

References

  1. Ames BN and Dubin DT (1960) The role of polyamines in the neutralization of bacteriolphage deoxyribonucleic acid. J. Biol Chem 235:769–775.

    Google Scholar 

  2. Anderson JM and Boardman NK (1966) Fractionation of the photochemical systems of photosynthesis. Biochim Biophys Acta 112:403–412.

    Google Scholar 

  3. Anderson MM and McCarty RE (1972) Rapid and sensitive assay for free fatty acids using Rhodamine 6G. Anal Biochem 45:260–270.

    Google Scholar 

  4. Anderson MM, McCarty RE and Zimmer EA (1974) The role of galactolipids in spinach chloroplast lamellar membranes. I. Partial purification of a bean galactolipid lipase and its action on subchloroplast particles. Plant Physiol 53:699–704.

    Google Scholar 

  5. Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15.

    Google Scholar 

  6. Bamberger ES and Park RB (1966) Effect of hydrolytic enzymes on the photosynthetic efficiency and morphology of chloroplasts. Plant Physiol 41:1591–1600.

    Google Scholar 

  7. Baszyński T (1974) Effect of α-tocopherol on reconstitution of photosystem I in heptane-extracted spinach chloroplasts. Biochim Biophys Acta 347:31–35.

    Google Scholar 

  8. Baszyński T, Ruszkowska M, Król M, Tukendorf A and Wolińska D (1978) The effect of copper deficlency on the photosynthetic apparatus of higher plants. Z Pflanzenphysiol 89:207–216.

    Google Scholar 

  9. Böger P, Black CC and San Pietro A (1966) Photosynthetic reactions with pyridine nucleotide and 3-pyridinealdehyde-deamino-diphosphopyridine nucleotide and 3-pyridinealdehyde-deamino-diphosphopyridine nucleotide. Arch Biochem Biophys 115:35–43.

    Google Scholar 

  10. Böhme H (1978) Reaction of antibodies against ferredoxin, ferredoxin-NADP+ reductase and plastocyanin with spinach chloroplasts. Eur J Biochem 84:87–93.

    Google Scholar 

  11. Brand J, Baszyński T, Crane FL and Krogman DW (1972) Selective inhibition of photosynthetic reactions by polycations. J Biol Chem 247:2814–2819.

    Google Scholar 

  12. Brand J, San Pietro A and Mayne BC (1972) Site of polylysine inhibition of photosystem I in spinach chloroplasts. Arch Biochem Biophys 152:426–428.

    Google Scholar 

  13. Brody SS, Brody M and Döring G (1970) Effects of linolenic acid on system II and system I-associated light induced changes in absorption of chloroplasts. Z Naturforsch 25 b:367–372.

    Google Scholar 

  14. Elstner E, Pistorius E, Böger P and Trebst A (1968) Zur Role von Plastocyanin und Cytochrom f in photosynthetischen Elektronen-transport. Planta 79:146–161.

    Google Scholar 

  15. Hauska GA, McCarty RE, Berzborn RJ and Racker E (1971) Determination of plastocyanin content in chloroplasts and subchloroplast particles. J Biol Chem 246:3524–3531.

    Google Scholar 

  16. Kates M (1972) Techniques in lipidology. In Work TS et al., eds. Techniques in biochemistry and molecular biology, pp 393–469 Amsterdam: Elsevier/North Holland.

    Google Scholar 

  17. Katoh S (1971) Plastocyanin. In San Pietro A, ed. Methods in enzymology, Vol 23 A, pp 408–413. New York: Academic Press.

    Google Scholar 

  18. Krupa Z and Baszyński T (1975) Requirement of galactolipids for photosystem I activity in lyophilized spinach chloroplasts. Biochim Biophys Acta 408:26–34.

    Google Scholar 

  19. Krupa Z and Baszyński T (1977) Participation of sulphoquinovosyl diacylglycerol in the reconstitution of photosystem I activity of heptane-extracted chloroplasts. Bull Acad Pol Sci. Ser Biol 25:409–413.

    Google Scholar 

  20. Krupa Z and Baszyński T (1978) The effect of exogenous lipids on reconstitution of heptane-extracted spinach chloroplasts. Bull Acad Pol Sci, Ser Biol 26:885–889.

    Google Scholar 

  21. Michalski WP and Kaniuga Z (1980) Photosynthetic apparatus in chilling-sensitive plants. VII. Comparison of the effect of galactolipase treatment of chloroplasts and cold-dark storage of leaves on photosynthetic electron flow. Biochim Biophys Acta 589:84–99.

    Google Scholar 

  22. Nelson WL, Claccio EI and Hess GP (1961) A rapid method for the quantitative assay of proteolytic enzymes. Anal Biochem 2:39–44.

    Google Scholar 

  23. Plesničar M and Bendall DS (1970) The plastocyanin content of chloroplasts from some higher plansts estimated by a sensitive enzymatic assay. Biochim Biophys Acta 216:192–199.

    Google Scholar 

  24. Pohl P, Glasl H and Wagner H (1970) Zur Analytik pflanzlicher Glyko- und Phospholipoide und ihrer Fettsäuren. I. Eine neue dunnschichtchromatographísche Methode zur Trennung pflanzlicher Lipoide und quantitativen Bestimmung ihrer Fettsäure-Zusammensetzung. J Chromatog 49:488–492.

    Google Scholar 

  25. Racker E, Hauska GA, Lien S, Berzborn RJ and Nelson N (1972) Resolution and reconstitution of the system of photophosphorylation. In Forti G et al., eds., Proceedings of the 2nd International Congress on Photosynthesis, Vol 2, pp 1097–1113. The Haque: Dr W Junk Publishers.

    Google Scholar 

  26. Radunz A (1980) Asymmetric distribution of lipids and proteins in the thylakoid membranes. A study with specific antisera. In: Abstracts of the 5th International Congress on Photosynthesis, p 460. Halkidiki, Greece.

  27. Ramshaw JAM, Brown RH, Scawen MD and Boulter D (1973) Higher plant plastocyanin. Biochim Biophys Acta 303:269–273.

    Google Scholar 

  28. Rawyler A and Siegenthaler PA (1980) Role of lipids in functions of photosynthetic membranes revealed by treatment with lipolytic acyl hydrolase. Eur J Biochem 110:179–187.

    Google Scholar 

  29. Rémy R (1971) Resolution of chloroplast lamellar proteins by electrophoresis in polyacrylamide gels. Different patterns obtained with fractions enriched in either chlorophyll a or chlorophyll b. FEBS Lett 13:313–317.

    Google Scholar 

  30. Roughan PG and Batt RD (1968) Quantitative analysis of sulfolipid (sulfoquinovosyl diglyceride) and galactolipids (monogalactosyl and digalactosyl diglycerides) in plant tissues. Anal Biochem 22:74–88.

    Google Scholar 

  31. Sane PV, Goodchild DJ and Park RB (1970) Characterization of chloroplast photosystem 1 and 2 separated by a non-detergent method. Biochim Biophys Acta 216:162–178.

    Google Scholar 

  32. Schacterle GR and Pollack RL (1973) A simplified method for the quantitative assay of small amounts of protein in biological material. Anal Biochem 51:654–655.

    Google Scholar 

  33. Schmid GH, Radunz A and Menke W (1975) The effect of an antiserum to plastecyanin on various chloroplast preparations. Z Naturforsch 30 c: 201–212.

    Google Scholar 

  34. Selman BR, Johnson GL, Dilley RA and Voegell KK (1975) Location of plastocyanin in chloroplast membranes. In Avron M, ed. Proceedings of the 3rd International Congress on Photosynthesis, Vol 1. pp 887–909. Amsterdam: Elsevier.

    Google Scholar 

  35. Siegenthaler PA (1973) Change in pH dependence and sequential inhibition of photosynthetic activity in chloroplasts by unsaturated fatty acids. Biochim Biophys Acta 305:152–163.

    Google Scholar 

  36. Wintermans JFGM, Helmsing PJ, Polman BJJ, van Gisbergen J and Collard J (1969) Galactolipid transformations and photochemical activities of spinach chloroplasts. Biochim Biophys Acta 189:95–105.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Biology, Maria Curie-Sklodowska University, Lublin, Poland

    Z. Krupa

Authors
  1. Z. Krupa
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Krupa, Z. The action of lipases on chloroplast membranes I. The release of plastocyanin from galactolipase-treated thylakoid membranes . Photosynth Res 3, 95–104 (1982). https://doi.org/10.1007/BF00040707

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00040707

Key words

Search

Navigation