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Molecular analysis of two Brassica napus genes expressed in the stigma

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Abstract

A partial cDNA clone, Pis 63, corresponding to a mRNA highly expressed in Brassica napus pistils, was isolated by differential screening. PCR was used to complete the Pis 63 sequence (Pis 63-1) and to obtain the sequence of another related cDNA (Pis 63-2). Northern blot and in situ analyses demonstrated that these transcripts are expressed in the stigma throughout flower development. Pis 63-1 and Pis 63-2 display similarity to a cotton fibre cDNA clone.

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Robert, L.S., Allard, S., Gerster, J.L. et al. Molecular analysis of two Brassica napus genes expressed in the stigma. Plant Mol Biol 26, 1217–1222 (1994). https://doi.org/10.1007/BF00040703

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  • DOI: https://doi.org/10.1007/BF00040703

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