Abstract
cDNA clones encoding the precursor of the Rieske FeS protein of the chloroplast thylakoid membrane have been isolated from a pea leaf cDNA library in λgt 11, following screening with antibodies to purified pea chloroplast Rieske FeS protein. The longest cDNA insert of 880 bp encodes a polypeptide of 230 amino acid residues, of which 50 residues constitute an N-terminal cleavable presequence and 180 residues make up the mature protein. Transcription and translation of the cDNA in vitro produced a polypeptide of 26 kDa which was efficiently imported by isolated pea chloroplasts and processed to the mature 20 kDa protein. Southern hybridisation to pea genomic DNA indicated the presence of a single gene encoding the Rieske FeS protein in the haploid genome.
References
Alt J, Westhoff P, Sears BB, Nelson N, Hurt E, Hauska G, Herrmann RG: Genes and transcripts for the polypeptides of the cytochrome b 6/f complex from spinach thylakoid membranes. EMBO J 2: 979–986 (1983).
Bartling D, Clausmeyer S, Oelmüller R, Herrmann RG: Towards epitope models for chloroplast transit sequences. Bot Mag, Tokyo (special issue) 2: 119–144 (1990).
Britt RD, Sauer K, Klein MP, Knaff DB, Kriauciunas A, Yu C-A, Yu L, Malkin R: Electron spin echo envelope modulation spectroscopy supports the suggested coordination of two histidine ligands to the Rieske Fe-S centers of the cytochrome b 6f complex of spinach, and the cytochrome bc 1complexes of Rhodospirillum rubrum, Rhodobacter sphaeroides R-26, and bovine mitochondria. Biochemistry 30: 1892–1901 (1991).
Cavener DR, Ray SC: Eukaryotic start and stop translation sites. Nucl Acids Res 19: 3185–3192 (1991).
Gantt JS, Key JL: Isolation of nuclear encoded plastid ribosomal protein cDNAs. Mol Gen Genet 202: 186–193 (1986).
Haley J, Bogorad L: A 4 kDa maize chloroplast polypeptide associated with the cytochrome b 6-f complex: subunit 5, encoded by the chloroplast petE gene. Proc Natl Acad Sci USA 86: 1534–1538 (1989).
Hauska G, Nitschke W, Herrmann RG: Amino acid identities in the three redox center-carrying polypeptides of the cytochrome bc 1-b6f complexes. J Bioenerg Biomembr 20: 211–230 (1989).
Heinemeyer W, Alt J, Herrmann RG: Nucleotide sequence of the clustered genes for apocytochrome b6 and subunit 4 of the cytochrome b/f complex in the spinach plastid chromosome. Curr Genet 8: 543–549 (1984).
Hurt E, Hauska G, Malkin R: Isolation of the Rieske iron-sulfur protein from the cytochrome b 6-f complex of spinach chloroplasts. FEBS Lett 134: 1–5 (1981).
Kallas T, Spiller S, Malkin R: Primary structure of cotranscribed genes encoding the Rieske FeS and cytochrome f proteins from the cyanobacterium Nostoc PCC 7906. Proc Natl Acad Sci USA 85: 5794–5798 (1988).
Kozak M: Compilation and analysis of sequence upstream from the translational start site in eukaryotic mRNAs. Nucl Acids Res 12: 857–872 (1987).
Lütcke HA, Chow KC, Mickel FS, Moss KA, Kern HF, Scheele GA: Selection of AUG initiation codons differs in plants and animals. EMBO J 6: 43–48 (1987).
Madueño F, Napier JA, Cejudo FJ, Gray JC: Import and processing of the precursor of the Rieske FeS protein of tobacco chloroplasts. Plant Mol Biol, in press.
Mayes SR, Barber J: Primary structure of the psbN-psbH-petC-petA gene cluster of the cyanobacterium Synechocystis PCC 6803. Plant Mol Biol 17: 289–293 (1991).
Newman BJ, Gray JC: Characterisation of a full-length cDNA clone for pea ferredoxin-NADP+ reductase. Plant Mol Biol 10: 511–520 (1988).
Palomares R, Herrmann RG, Oelmüller R: Different blue-light requirement for the accumulation of transcripts from nuclear genes for thylakoid proteins in Nicotiana tabacum and Lycopersicon esculentum. J Photochem Photobiol B Biol 11: 151–162 (1991).
Salter AH, Newman BJ, Gray JC: Characterization of cDNA clones encoding the pea chloroplast Rieske Fe-S protein. In: Barber J, Malkin R (eds) Techniques and New Developments in Photosynthesis Research, pp. 473–476. Plenum, New York (1989).
Salter AH, Gray JC: Characterisation of a full-length cDNA clone encoding the pea Rieske Fe-S protein: import and processing by isolated chloroplasts. In: Baltscheffsky M (ed) Current Research in Photosynthesis, vol 3, pp. 267–270. Kluwer, Dordrecht (1990).
Sanger F, Coulson AR, Barrell BG, Smith AJH, Roe BA: Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol 143: 161–178 (1980).
Steppuhn J, Rother C, hermans J, Jansen T, Salnikow J, Hauska G, Herrmann RG: The complete amino-acid sequence of the Rieske FeS-precursor protein from spinach chloroplasts deduced from cDNA analysis. Mol Gen Genet 210: 171–177 (1987).
Tittgen J, Hermans J, Steppuhn J, Jansen T, Jansson C, Andersson B, Nechushtai R, Nelson N, Herrmann RG: Isolation of cDNA clones for fourteen nuclear-encoded thylakoid membrane proteins. Mol Gen Genet 204: 258–265 (1986).
von Heijne G, Steppuhn J, Herrmann RG: Domain structure of mitochondrial and chloroplast targeting peptides. Eur J Biochem 180: 535–545 (1989).
Wahl GM, Stern M, Stark GR: Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl paper and rapid hybridisation by using dextran sulphate. Proc Natl Acad Sci USA 76: 3683–3687 (1979).
Widger W: The cloning and sequencing of Synechococcus sp. PCC 7002 petCA operon: implications for the cytochrome c-553 binding domain of cytochrome f. Photosynth Res 30: 71–84 (1991).
Willey DL, Auffret A, Gray JC: Structure and topography of cytochrome f in pea chloroplast membranes. Cell 36: 555–562 (1984).
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Salter, A.H., Newman, B.J., Napier, J.A. et al. Import of the precursor of the chloroplast Rieske iron-sulphur protein by pea chloroplasts. Plant Mol Biol 20, 569–574 (1992). https://doi.org/10.1007/BF00040617
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DOI: https://doi.org/10.1007/BF00040617