Abstract
The genes encoding the photosynthetic cytochrome b 6 (petB) and subunit 4 (petD) have been cloned and sequenced from the unicellular, photoheterotrophic, transformable cyanobacterium Synechococcus sp. PCC 7002, formerly designated Agmenellum quadruplicatum. The gene arrangement was found to be similar to that reported in the cyanobacterium Nostoc PCC 7906. The DNA and derived protein sequences were compared to chloroplast and the other cyanobacterial sequences. By pulsed-field electrophoresis, the petBD operon and the petCA operon, encoding the Rieske iron-sulfur protein and cytochrome f, were found to be located on separate, unlinked,Not I-digested DNA fragments. ThepetBD operon was found on the third largest Not I fragment (NC-325) while the petCA operon was found on the second largest Not I fragment (NB-370). These results suggest the two operons are not in proximity. The 1.35 kb transcript was shown to be light-regulated. Transcripts from cells grown under constant illumination showed a decrease in petB transcript levels to undetectable levels within 2 h after the cells were placed in the dark. Upon reillumination, transcript levels rose to three-fold over that seen initially under constant illumination.
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References
Anderson SL, McIntosh L: Light-activated heterotrophic growth of the cyanobacterium Synechocystis sp. strain PCC 6803: a blue-light-requiring process. J Bact 173: 2761–2767 (1991).
Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K: Current Protocols in Molecular Biology. Greene Publishers/Wiley-Interscience, New York (1989).
Bancroft I, Wolk CP, Oren EV: Physical and genetic maps of the genome of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120. J Bact 171: 5940–5948 (1989).
Binder BJ, Chisholm SW: Relationship between DNA cycle and growth rate in Synechococcus sp. Strain PCC 6301. J Bact 172: 2313–2319 (1990).
Burks C, Fickett JW, Goad WB, Kanehisa M, Lewitter FI, Rindone WP, Swindell CD, Tung CS, Bilofsky HS: The GenBank nucleic acid sequence database. CABIOS 1: 225–233 (1985).
Bustos SA, Schaefer MR, Golden SS: Different and rapid responses of four cyanobacterial psbA transcripts to changes in light intensity. J Bact 172: 1998–2004 (1990).
Chu G, Vollrath D, Davis RW: Separation of large DNA molecules by contour-clamped homogeneous electric fields. Science 234: 1582–1585 (1986).
Cramer WA, Black MT, Widger WR, Girvin ME: Structure and function of photosynthetic cytochrome b/c1 and b 6f complexes. In: Barber J (ed) Electron Transfer Mechanism and Oxygen Evolution, pp. 447–492. Elsevier, Amsterdam (1987).
Cramer WA, Crofts AR: Electron and proton transport. In: Photosynthesis: Energy Conservation by Plants and Animals, Vol. 1, p. 387–467. Academic Press, New York (1983).
Crofts AR: The mechanism of ubiquinol: cytochrome c oxido-reductases of mitochondria and Rb. sphaeroides. In: Mortonisi A (ed) The Enzymes of Biological Membranes, pp. 347–382 (1985).
Crofts AR, Meinhardt SW, Jones KR, Snozzi M: The role of the quinone pool in the cyclic electron transfer chain of Rb. Sphaeroides. Biochim Biophys Acta 723: 202–218 (1983).
De Lorimier R, Bryant DA, Porter RD, Liu W-Y, Jay E, StevensJr SE: Genes for the α and β subunits of phycocyanin. Proc Natl Acad Sci USA 81: 7946–7650 (1984).
Devereux J, Haeberli P, Smithies O: A comprehensive set of sequence analysis programs for the VAX. Nucl Acids Res 12: 387–395 (1984).
Feinberg AP, Vogelstein B: A technique for radiolabeling endonuclease fragments to high specific activity. Anal Biochem 132: 6 (1983).
Frei R, Binder A, Bachofen R: Respiration in energy-transducing membranes of the thermophilic cyanobacterium Mastigocladus laminosus II. Oxidative phosphorylation. Biochim Biophys Acta 765: 247–252 (1984).
Furbacher PN, Girvin ME, Cramer WA: On the question of interheme electron transfer in the chloroplast cytochrome b 6 in situ. Biochemistry 28: 8990–8998 (1989).
Heinemeyer A, Alt J, Herrmann RG: Nucleotide sequence of the clustered genes for apocytochrome b 6and subunit 4 of the cytochrome b/f complex in the spinach plastid chromosome. Curr Genet 8: 543–549 (1984).
Kallas T, Spiller S, Malkin R: Characterization of two operons encoding the cytochrome b 6-f complex of the cyanobacterium Nostoc PCC 7906. J Biol Chem 263 (1988).
Kallas T, Spiller S, Malkin R: Primary structure of contranscribed genes encoding the Rieske Fe-S and cytochrome fproteins of the cyanobacterium Nostoc PCC 7906. Proc Natl Acad Sci USA 85: 5794–5798 (1988).
Michaels A, Herrin DL: Translational regulation of chloroplast gene expression during the light-dark cell cycle of Chlamydomonas: Evidence for control by ATP/energy supply. Biochem Biophys Res Commun 170: 1082–1088 (1990).
Mohamed A, Jansson C: Influence of light accumulation of photosynthesis-specific transcripts in the cyanobacterium Synechocystis 6803. Plant Mol Biol 13: 693–700 (1989).
Mohamed A, Jansson C: Photosynthetic electron transport controls degradation but not production of psbA transcripts in the cyanobacterium Synechocystis 6803. Plant Mol Biol 16: 891–897 (1991).
Pallotta D, Bernier F, Hamelin M, Martel R, Lemieux G: cDNA cloning of Physarum polycephalum stage-specific mRNAs. In: Dove DF, Dee J, Hatano S, Haugli FB, Wohlfarth-Bottermann K-E (eds) The Molecular Biology of Physarum polycephalum, pp. 315–328. Plenum Press, New York/London (1986).
Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).
Schubert K, Liese F, Rumberg B: Analysis of the variability of the H/e stoichiometry in chloroplasts. In: Baltscheffsky M (ed) Current Research in Photosynthesis, pp. 279–282. Kluwer Academic Publishers, Dordrecht (1990).
Sinning I, Koepke J, Schiller B, Mathis P, Rutherford AW, Michel H: The herbicide resistant mutant T1 from Rps. viridis: Altered herbicide binding and three dimensional structure. In: Baltscheffsky M (ed) Current Research in Photosynthesis, pp. 173–176. Martinus Nijhoff, Dordrecht (1990).
Slovacek RE, Mills JD, Hind G: The function of cyclic electron transport in photosynthesis. FEBS Lett 87: 73–76 (1978).
Smart LB, McIntosh L: Expression of photosynthesis genes in the cyanobacterium Synechocystis sp. PCC 6803: psaA-psaB and psbA transcripts accumulate in dark grown cells. Plant Mol Biol 17: 959–971 (1991).
Smith CL, Cantor CR: Purification, specific fragmentation, and separation of large DNA molecules. Meth Enzymol 155: 449–467 (1987).
Stevens SE, Patterson COP, Myers J: The production of hydrogen peroxide in blue by blue-green algae: a survey. J Phycol 9: 427–430 (1973).
Szczepaniak A, Cramer WA: On the topography and function of the chloroplast b 6-f complex. Zeitschr Naturforsch 44c: 123–131 (1989).
Widger W: The cloning and sequencing of the Synechococcus sp. PCC 7002 petCA operon: Implications for the cytochrome c553 binding domain of cytochrome f. Photosynth Res 30: 71–84 (1991).
Widger WR, Cramer WA: The cytochrome b 6/f complex. In: Bogarad L (ed) Cell Culture and Somatic Cell Genetics of Plants, vol. 7B, pp. 149–176. Academic Press, New York (1991).
Widger WR, Cramer WA, Herrmann RG, Trebst A: Sequence homology and structural similarity between cytochrome b of mitochondrial complex III and the chloroplast b 6-f complex: Position of the cytochrome b hemes in the membrane. Proc Natl Acad Sci USA 81: 674–678 (1984).
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Brand, S.N., Tan, X. & Widger, W.R. Cloning and sequencing of the petBD operon from the cyanobacterium Synechococcus sp. PCC 7002. Plant Mol Biol 20, 481–491 (1992). https://doi.org/10.1007/BF00040607
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DOI: https://doi.org/10.1007/BF00040607