Skip to main content
Log in

The gene for ribosomal protein L27 is located on the plasted rather than the nuclear genome of the chlorophyll c-containing alga Pleurochrysis carterae

  • Short Communication
  • Published:
Plant Molecular Biology Aims and scope Submit manuscript

Abstract

The gene for ribosomal protein L27 (rpl27) has not been found in plastid genomes. We report here that the rpl27 gene is located in the plastid genome of the prymnesiophyte Pleurochrysis carterae. The deduced amino acid sequence showed 59% identity with E. coli L27. 1.0 kb transcript of the gene was detected by Northern blot analysis. Nucleotide sequence analysis of PCR products suggested that rpl27 is widespread in the genomes of Prymesiophyta and Rhodophyta. In all species of Prymnesiophyta examined in this study, the gene is located at the 3′ downstream region of Rubisco operon.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Assali N-E, Martin WF, Sommerville CC, Loiseaux-de Goer S: Evolution of the Rubisco operon from prokaryotes to algae: Structure and analysis of the rbcS gene of the brown alga Pylaiella littoralis. Plant Mol Biol 17: 853–863 (1991).

    PubMed  Google Scholar 

  2. Boczar BA, Delaney TP, Cattolico RA: Gene for the ribulose-1,5-bisphosphate carboxylase small subunit protein of the marine chromophyte Olisthodiscus luteus is similar to that of a chemoautotrophic bacterium. Proc Natl Acad Sci USA 86: 4996–4999 (1989).

    PubMed  Google Scholar 

  3. Bryant DA, Stirewalt VL: The cyanelle genome of Cyanophora paradoxa encodes ribosomal proteins not encoded by the chloroplast genomes of higher plants. FEBS Lett 259: 273–280 (1990).

    Article  PubMed  Google Scholar 

  4. Chen R, Mende L, Arfsten U: The primary structure of protein L27 from the peptidyl-tRNA binding site of Escherichia coli ribosomes. FEBS Lett 59: 96–99 (1975).

    Article  PubMed  Google Scholar 

  5. Douglas SE, Durnford DG: The small subunit of ribulose-1,5-bisphosphate carboxylase is plastid-encoded in the chlorophyll c-containing alga Cryptomonas Φ. Plant Mol Biol 13: 13–20 (1989).

    PubMed  Google Scholar 

  6. Fearon K, Mason TL: Structure and regulation of a nuclear gene in Saccharomyces cerevisiae that specifies MRP7, a protein of the large subunit of the mitochondrial ribosome. Mol Cell Biol 8: 3636–3646 (1988).

    PubMed  Google Scholar 

  7. Ferrari FA, Trach K, Hoch JA: Sequence analysis of the spoOB locus reveals a polycistronic transcription unit. J Bact 161: 556–562 (1985).

    PubMed  Google Scholar 

  8. Fujiwara S, Iwahashi H, Someya J, Nishikawa S, Minaka N: Structure and cotranscription of the plastid-encoded rbcL and rbcS genes of Pleurochrysis carterae (Prymnesiophyta). J Phycol 29: 347–355 (1993).

    Article  Google Scholar 

  9. Hwang S-R, Tabita FR: Cotranscription, deduced primary structure, and expression of the chloroplast-encoded rbcL and rbcS genes of the marine diatom Cylindrotheca sp. strain Nl. J Biol Chem 266: 6271–6279 (1991).

    PubMed  Google Scholar 

  10. Hwang S-R, Tabita FR: Acyl carrier protein-derived sequence encoded by the chloroplast genome in the marine diatom Cylindrotheca sp. strain Nl. J Biol Chem 266: 13492–13494 (1991).

    PubMed  Google Scholar 

  11. Kessler U, Maid U, Zetsche K: An equivalent to bacterial ompR genes is encoded on the plastid genome of red algae. Plant Mol Biol 18: 777–780 (1992).

    Article  PubMed  Google Scholar 

  12. Kostrzewa M, Valentin K, Maid U, Radetzky R, Zetsche K: Structure of the rubisco operon from the multicellular red alga Antithamnion spec. Curr Genet 18: 465–469 (1990).

    PubMed  Google Scholar 

  13. Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1982).

    Google Scholar 

  14. Michalowski CB, Flachmann R, Löffelhardt W, Bohnert HJ: Gene nadA, encoding quinolinate synthetase, is located on the cyanelle DNA from Cyanophora paradoxa. Plant Physiol 95: 329–330 (1991).

    Google Scholar 

  15. Michalowski CB, Löffelhardt W, Bohnert HJ: An ORF323 with homology of crtE, specifying prephytoene pyrophosphate dehydrogenase, is encoded by cyanelle DNA in the eukaryotic alga Cyanophora paradoxa. J Biol Chem 266: 11866–11870 (1991).

    PubMed  Google Scholar 

  16. Neumann-Spallart C, Brandtner M, Kraus M, Jakowitsch J, Bayer MG, Maier TL, Schenk HEA, Löffelhardt W: The petFI gene encoding ferredoxin I is located close to the str operon on the cyanelle genome of Cyanophora paradoxa. FEBS Lett 268: 55–58 (1990).

    Article  PubMed  Google Scholar 

  17. Ofengand J, Ciesiolka J, Denman R, Nurse K: Structural and functional interactions of the tRNA-ribosome complex. In: Hardesty B, Kramer G (eds) Structure, Function, and Genetics of Ribosomes, pp. 473–494. Springer-Verlag, New York (1986).

    Google Scholar 

  18. Reith M, Munholland J: An hsp70 homolog is encoded on the plastid genome of the red alga, Porphyra umbilicalis. FEBS Lett 294: 116–120 (1990).

    Article  Google Scholar 

  19. Reith M: psaE and trnS (CGA) are encoded on the plastid genome of the red alga Porphyra umbilicalis. Plant Mol Biol 18: 773–775 (1992).

    Article  PubMed  Google Scholar 

  20. Scaramuzzi C: Heat shock hsp70 protein is chloroplast encoded in the chromophytic alga, Pavlova lutherii. J Phycol 27s: 65 (1991).

    Google Scholar 

  21. Valentin K, Zetsche K: The genes of both subunits of ribulose-1,5-bisphosphate carboxylase constitute an operon on the plastome of a red alga. Curr Genet 16: 203–209 (1989).

    PubMed  Google Scholar 

  22. Valentin K, Zetsche K: Structure of the Rubisco operon from the unicellular red alga Cyanidium caldarium: evidence for a polyphyletic origin of the plastids. Mol Gen Genet 222: 425–430 (1990).

    PubMed  Google Scholar 

  23. Valentin K, Zetsche K: Rubisco genes indicate a close phylogenetic relation between the plastids of Chromophyta and Rhodophyta. Plant Mol Biol 15: 575–584 (1990).

    PubMed  Google Scholar 

  24. Wang S, Liu X-Q: The plastid genome of Cryptomonas Φ encodes an hsp70-like protein, a histone-like protein, and acyl carrier protein. Proc Natl Acad Sci USA 88: 10783–10787 (1991).

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fujiwara, S., Kawachi, M., Inouye, I. et al. The gene for ribosomal protein L27 is located on the plasted rather than the nuclear genome of the chlorophyll c-containing alga Pleurochrysis carterae . Plant Mol Biol 24, 253–257 (1994). https://doi.org/10.1007/BF00040594

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00040594

Key words

Navigation