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Localisation of cis elements in the promoter of a wheat α-Amy2 gene

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Abstract

A functional analysis of the promoter from the wheat α-amylase gene α-Amy2/54 is described. Mutant α-Amy2/54 promoters containing replacements or deletions were constructed and their ability to direct expression of the reporter gene β-glucuronidase (GUS) in gibberellin-responsive oat aleurone protoplasts analysed. Chimaeric promoters using regions of the cauliflower mosaic virus (CaMV) 35S and α-Amy2/54 promoters were also analysed. The results suggest that at least three regions within the α-Amy2/54 promoter contain cis elements that are necessary for high-level gibberellin-regulated transcription. Fusion of 1.8 kb of promoter sequence upstream from −117 bp to a minimal (−55 CaMV 35S) promoter gave rise to hormone-independent expression implying that the region 3′ to −117 bp contains an element which represses transcription in the absence of gibberellin or presence of abscisic acid.

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Authors and Affiliations

  1. Dept of Agricultural Sciences, University of Bristol, AFRC Institute of Arable Crops Research, Long Ashton Research Station, BS18 9AF, Bristol, UK

    A. K. Huttly, A. L. Phillips & J. W. Tregear

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  1. A. K. Huttly
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  2. A. L. Phillips
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  3. J. W. Tregear
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Huttly, A.K., Phillips, A.L. & Tregear, J.W. Localisation of cis elements in the promoter of a wheat α-Amy2 gene. Plant Mol Biol 19, 903–911 (1992). https://doi.org/10.1007/BF00040523

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  • DOI: https://doi.org/10.1007/BF00040523

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