Abstract
Sugar constituents of the fucose-containing polysaccharides (FCPs) from 21 species of brown algae were analyzed. FCPs were extracted with hot water (100 °C, 4 h), separated by precipitation with 20% (v:v) ethanol in the presence of 0.05 M MgCl2 to remove contaminating soluble alginate, and purified by DEAE-Sephadex column chromatography. The samples were hydrolyzed with HCI, and neutral sugar and uronic acid were separated by anion exchange chromatography. Their amounts were determined by gas-liquid chromatography. The neutral sugars in the FCPs from Ishige okamurae, Laminaria ochotensis, Myelophycus simplex, Padina arborescens and Sargassum thunbergii all contained arabinose, fucose, galactose, glucose, mannose, rhamnose and xylose residues. The FCPs from Ishige okamurae, Padina arborescens, Sargassum hemiphyllum, S. patents and S. sagamianum contained the four uronic acids, galacturonic acid, glucuronic acid, guluronic acid and mannuronic acid.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bird, G. M. & P. Hass, 1931. On the nature of the cell wall constituents of Laminaria spp. mannuronic acid. Biochem. J. 25: 403–411.
Dubois, M., K. A. Gilles, J. K. Hamilton, P. A. Rebers & F. Smith, 1956. Colorimetric method for determination of sugars and related substances. Analyt. Chem. 28: 350–356.
Hoagland, D. R. & L. L. Lieb, 1915. The complex carbohydrates and forms of sulphur in marine algae of the Pacific coast. J. biol. Chem. 23: 287–297.
Imanari, T., Y. Arakawa & Z. Tamura, 1969. Gas chromatographic analysis of aldoses. Chem. Pharm. Bull. 17: 1967–1969.
Kylin, K., 1913. Zur Biochemie der Meeresalgen. HoppeSeyler's Z. Physiol. Chem. 83: 171–197.
Larsen, B. & A. Haug, 1961. Separation of uronic acids on anion exchange columns. Acta chem. scared. 15: 1397–1398.
Larsen, B., A. Haug & T. Painter, 1970. Sulphated polysaccharides in brown algae 3. The native state of fucoidan in Ascophyllum nodosum and Fucus vesiculosus. Acta chem. scand. 24: 3339–3352.
Lunde, G., E. Heen & E. Öy, 1937. Über fucoidin. HoppeSeyler's Z. Physiol. Chem. 247: 189–196.
Miwa, T., 1940. Biochemische studien über die zellmembran von Braun- und rotalgen. Jap. J. Bot. 11: 41–128.
Nelson, W. L. & L. H. Cretcher, 1931. The carbohydrate acid sulfate of Macrocystis pyrifera. J. biol. Chem. 94: 147–154.
Nishide, E., H. Anzai & N. Uchida, 1987. A comparative investigation on the contents of fucose-containing polysaccharides from various Japanese brown algae. Nippon Suisan Gakkaishi 53: 1083–1088.
Percival, E. G. V. & A. G. Ross, 1950. Fucoidin. Part 1. The isolation and purification of fucoidin from brown seaweeds. J. chem. Soc. 1950: 717–720.
Perry, M. B. & R. K. Hulyalkar, 1965. The analysis of hexuronic acids in biological materials by gas-liquid partition chromatography. Can. J. Biochem. 43: 573–584.
Schmidt, M., 1962. Fractionation of acid mucopolysaccharides on DEAE-Sephadex anion exchanger. Biochim. Biophys. Acta 63: 346–348.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nishide, E., Anzai, H., Uchida, N. et al. Sugar constituents of fucose-containing polysaccharides from various Japanese brown algae. Hydrobiologia 204, 573–576 (1990). https://doi.org/10.1007/BF00040289
Issue Date:
DOI: https://doi.org/10.1007/BF00040289