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Fertile plants regenerated from suspension culture-derived protoplasts of an indica type rice (Oryza sativa L.)

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Abstract

Calluses were induced from immature embryos of an indica type rice and finely dispersed cell suspension cultures were initiated from the callus using modified AA medium (S1 medium). The suspension cultures were maintained alternatively (1–2 passages in each medium) in S1 medium and S2 medium, the latter containing KNO3, NH4NO3, proline and glutamine as nitrogen source. Protoplasts of high quality were isolated form suspension cells cultured in S2 medium supplemented with ABA. Embedding the protoplasts in agarose blocks containing NH4NO3-free modified KM8P(PM1) medium and immersing the blocks in NH4NO3-containing modified KM8P(PM3) medium were most effective for obtaining protoplast division and callus formation. The protoplast-derived calluses were precultured in potato extract-aand/or ABA-containing N6(D1, D2 or D3) media and many embryo-like structures were formed. These structures developed into plantlets after being transferred to N6 differentiation (D4) medium. The regenerated plantlets grew into mature plants and beard seeds normally.

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Abbreviations

AA medium:

amino acids based medium

ABA:

abscisic acid

BA:

benzyladenine

2,4-D :

2,4-dichlorophenoxyacetic acid

DF:

division frequency

IAA:

indoleacetic acid

KIN:

kinetin

NAA:

naphthaleneacetic acid

PE:

planting efficiency

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Author notes

  1. Yanhai Yin

    Present address: MRC7, Division of Plant Biology, Department of Cell Biology, The Scripps Research Institute, 10666 North Torrey Pines Road, 92037, La Jolla, CA, USA

Authors and Affiliations

  1. Institute of Genetics, Academia Sinica, 100101, Beijing, China

    Yanhai Yin, Shizhong Li, Yiming Chen, Hongqing Guo, Wenzhong Tian, Ying Chen & Liangcai Li

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  1. Yanhai Yin
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  2. Shizhong Li
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  3. Yiming Chen
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  6. Ying Chen
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  7. Liangcai Li
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Yin, Y., Li, S., Chen, Y. et al. Fertile plants regenerated from suspension culture-derived protoplasts of an indica type rice (Oryza sativa L.). Plant Cell Tiss Organ Cult 32, 61–68 (1993). https://doi.org/10.1007/BF00040117

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  • DOI: https://doi.org/10.1007/BF00040117

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