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The use of embryo culture for the recovery of plants from cassava (Manihot esculenta Crantz) seeds

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Abstract

Cassava fertility and seed viability are frequently low, which can be a disadvantage in a breeding programme. An embryo culture method is described whereby embryonic axes are excised from mature seeds and placed on a culture medium containing 1.23 μM indolebutyric acid (IBA) at 30°C under continuous light. The number of plants recovered by embryo culture was much greater than the number recovered from conventional seed germination procedures.

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References

  1. Capinpin JM and Bruce VC (1955) Floral biology and cytology of Manihot ultilissima. Phillippine Agric 39: 306–316

    Google Scholar 

  2. Collins GB and Grosser JW (1984) Culture of embryos. In: Vasil IK (ed) Cell culture and somatic cell genetics of plants. Vol. 1. Laboratory procedures and their applications. New York: Academic Press pp 241–257

    Google Scholar 

  3. Jennings DL (1963) Variation in pollen and ovule fertility in varieties of cassava, and the effect of interspecific crossing on fertility. Euphytica 12: 69–76

    Google Scholar 

  4. Kartha KK, Gamborg OL, Constabel F and Shyluk JP (1974) Regeneration of cassava plants from apical meristems. Plant Sci Lett 2: 107–113

    Google Scholar 

  5. Martin F (1976) Cytogenetics and plant breeding of cassava; A review. Plant Breeding Abst 46: 909–916

    Google Scholar 

  6. Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Google Scholar 

  7. Nartey F, Moller BL and Anderson MR (1974) Changes in the major constituents of Manihot esculenta seeds during germination and growth. Econ Bot 28: 145–154

    Google Scholar 

  8. Roca WM (1984) Cassava. In: Sharp WR, Evans DA, Ammirato PV and Yamada Y (eds) Handbook of plant cell culture Vol. 2. Crop species. New York: Macmillan pp 269–301

    Google Scholar 

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Biggs, B.J., Smith, M.K. & Scott, K.J. The use of embryo culture for the recovery of plants from cassava (Manihot esculenta Crantz) seeds. Plant Cell Tiss Organ Cult 6, 229–234 (1986). https://doi.org/10.1007/BF00040008

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  • DOI: https://doi.org/10.1007/BF00040008

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