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Floral expression of a gene encoding an E2-relatedubiquitin-conjugating protein from Arabidopsis thaliana

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Abstract

An Arabidopsis thaliana gene (UBC6) encoding a homologue to ubiquitin-conjugating enzymes has been isolated which is capable of encoding a protein of 183 amino acids of ca. 21 kDa. Northern analysis indicates that the gene is expressed in flowers, seeds and, to a somewhat lesser extent, in 10-day seedlings but not in mature leaves, callus and pre-flowering plants. This pattern of expression is confirmed using transgenic Arabidopsis plants containing a UBC6 promoter-GUS gene fusion construct. These plants displey GUS activity in mature anthers prior to dehiscence, in developing embryos, sepals and the style after pollination.

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Authors and Affiliations

  1. Biochemistry Department, School of Biological Sciences, University of Sussex, Falmer, BN1 9QG, Brighton, East Sussex, UK

    Felicity Z. Watts, Neil Butt, Philip Layfield, Jesse Machuka & Anthony L. Moore

  2. Sussex Centre for Neuroscience, School of Biological Sciences, University of Sussex, Falmer, BN1 9QG, Brighton, East Sussex, UK

    Julian F. Burke

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  1. Felicity Z. Watts
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  2. Neil Butt
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  3. Philip Layfield
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  4. Jesse Machuka
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  5. Julian F. Burke
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  6. Anthony L. Moore
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Watts, F.Z., Butt, N., Layfield, P. et al. Floral expression of a gene encoding an E2-relatedubiquitin-conjugating protein from Arabidopsis thaliana . Plant Mol Biol 26, 445–451 (1994). https://doi.org/10.1007/BF00039553

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  • DOI: https://doi.org/10.1007/BF00039553

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